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CRIMIC CR01640

Gene Nrx-IV

CG6827

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3L:12148115..12148137	68F6	FBti0216165	92654

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCCAGGAGCAATACGAAAGCAGCATT	CGGGTGCAGATGTGTTTGCTCATTTC

Images

Construct Design Sequences

sgRNA sequence	TCTGGATTTCCGACACGACCTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GAGAGCGATCAAACGGTTGC	AAATCCAGACAGTTGCCCCT	ATCCGCATGCTTTTGTCACG	TGTGATGCAGTATGGCCAGA

 

Amplicon 5'

Amplicon 3'

GAGAGCGATCAAACGGTTGCTTACGAATAGCGGTGGCAAAACAAAAATGCCGCTTAACAACGCCCGTTTTTACTCGTTCGTTTTGAGCAAAGGCATTGGGGTTTTAGATTCTTGATTTCCGGACTTTTATGAAGCTTCCCGGGGGAGCAATTCGAAAATTTTGCCGGTTGCATCCCCAGTTGTGCTTGCCTTCTTTTCCGGCTTTTCAAATGCGCATGCGGCAAATAAGAAGCATCTGCTGATTGATGATGCTGTGTGAAACAGGTGATTTTATCATTATCCTTCCATATGTCAACAGACTTAAGCGCTTTTCCCCACAAATTTCCACATATCTTGAAAAATAAACAATCTATCGATGATATGCAAACATTTTTATGACTAAAAATAAACAATGCCGCGCAATTAATTTGCCTTGAAGCGTCGAAAGCAGACAGCCGACATATATCCTCCCAGACTTTGGTCGATTTCCATAATTTATTTCGGTTCTTATTGCCTCGAAAATAGCATCATTCGCACTTCGAGTTTCCCCCAGATGGACGGAAATGGCCTTTCAGACCTCGTCATATTTGTTTCATTTAGAACATTAGCCAAATTCTATGTATTTCGTTTTCTTTCTAATCACCATTGGGCAATTATGTAGCAAAAAGTAGTTCAAACTGGCTATATATGTATGTACATATGTTCCTGTGTTATTTACCAGAAGATTGTCATGCGTGTTTCCTCGGTTTCCTTTCGAGCTTATCAGGTTGATTTGTACCACTCCACCCAAGACTCAAAGTCAGCTTAAAGTGCCCACGAACTCCGAATGACCTCGCAAGGGTTGTTTTCCCCCACTCCGCTAAGAAAATGCCAGTGTGCTGTTTGCCTAGGAGGAGCTACTGCTGCACCCATTGTAAACAATTTCTTGGGGGTTGCGGCAGACAGGGGCAACTGTCTGGATTT

ATCCGCATGCTTTTGTCACGGATCCTAGGGGCTAAAAGACTCTCGTCAACGGAAAATATTATGGTGCAAATATGAGACAAGAATCTGCCTCTTAAAACAGTGGAACCATCTTAATAAGAAATATTTCTAACTAGTACCTAGTACTCTTAATACATGTTTAAGAGTAAAATATTCTAAATAAAGCAACTAATTACATCAGTTTATGTCCAATTTAATGCTGATTGTCTTAATTCATAGGAATTTTCCTTGGACTTCATGTGAAATTTAAAGATTCAATTGTATAGATTCTATTTATATGATATTTATTCATTACAAGCAAATTCAATATATCTTGTACGACTTAAATTACCCGGAAAACAGAGAACTAAGAACTCTTAAAATAAATAGCAAATATTTACTCCTCAGTCAATTTGTTTACTTCTTGCTGCCTTATAGATGTGGCTTTCTTTATGGGCCCATAAAAAGGAGGCCAATGATTATGTCCCGGCTGGAGTACTTCAGCTTATTGGCCGCCATCCAAACAGATTCTTCATATCTCAATTTTAATTGCGTCCGTGACAGTTTGCGTTTCATTTTTTCGCCCAACAATGTACACTCGTACATATATGGGTCATGGAATTCCGGATATTTATAAATCGATATTGCACTCTTTCAGACGCCTTCACGGACTATTTCTCCGACTACGATTGCAACCAGCCGCTGATGGAAAGAGCCGTCCTCACGGCCACATCATCCTTGACGGAAAGAGGACCCGACAAAGCACGACTAAACGGTGAGTAATATTGATTTTTTGTTGAAAGTTATTCGTTCGAAAGTTTAGTTGCCTTTCAGTTGTTTTATTAGCAATTATTATTTCGAAATGCTGCGCCAAAAATTCATAGGATAATATGTTTTTATTTTAATAGAACCTTTTTAAGTACACATTTTTATATATATACAGGTTATTAGATTGCTTTTTTTAAATGCTACATATAGCTATCCCATTTTTTTTTTTAAATTAAAGATAGTCATTATTTCTTAATTTTTTAAAATGAAAATGGGTACACCCCGCATTTGGTGGAGCAGTATATTTGCAGTATATTTATAAGGCGTAATCGCTGGGGGATTTTTACCCTCTCCAGTTCTGGCCATACTGCATCACA

 

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