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CRIMIC CR01645

Gene Ubr3

CG42593

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	X:7938957..7938959	7C9	FBti0210464	86477

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCTATCGCCAAACAGCTGATCGCAAG	ACGGTGCTTCCATGAAGAAGGCATAC

Images

Construct Design Sequences

sgRNA sequence	GTACATACGCCCCAGAACGCAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CAACAGCAATCAAGCGCACT	TCTGGGGCGTATGTACATGT	CGCAGGCCGAAGACAAAAA	GACCGAGGTGTCACCACAAT

 

Amplicon 5'

Amplicon 3'

CAACAGCAATCAAGCGCACTCTCTCGCACACACAGCACACTCACTACACACACATATGCACTGATTAGGGGAGTCAACTTTAGCCCAATTTAATCTCAACTGCTCATCTATTTGCAATGCTGTATTTGTGCATAATTTTCATCACTACTTATTACTTTTTGTGCCAGGCTGCGTATGTCGCCAAAGTTGCTTTTATCGTTTATGGTTGTTTGTCGCGTCTAGTCTTGTCTTGTCTTGTCCCACTTGTCAGCTCTGTCACACGCACACATACACACACTCGTGCAAACTGGCGGGCGGAGACGTACACGCACAGTGGAATGCAGCTTGCACCTTGTGGCTTTGTATCAGCTGTTTCGGTACGCCTTGCGTAATAAATGTTTCCGCAGCATCCGTCCCAAAACCACACGAACACGAATGTAGATGGTGCTTGATTATATGATGGCACCTTTTGTCACCGCTAGGTGCTCCTTTTTTTTTTTTGTGTTATATTTCTTTCACCTTTTTGTTTGTTACTCGTTGCAAGAACAATTGCAAAATTGCACTGGTGCGGAAAAAGTTGATTGACCGTCATTGTTGTTGATTTGACAAAAACACGCACAGTGGTCGCGAGGGGCGGATGTGATGTGGCCCACTGTAAGCTGTCAACGGCAATTGACCGAAAGACCGTTATGTTATTGGGTGGTGTGTATGTGTGTGCGTGCGTGCGTGTATGGGTGAGGGTGAGAGAGTGCACCTTTTTTCCGTTCCCATGTCATGTCACCTCCCCTTCCCTTCGCCACACCCTTGCTCCTCCATTCGGACCTTGTCCAAAAGTTCTCAGTTAGAAAGAGACTGCTGAGCCAAGATAGTGAGAGCGAGAGAGAGTTGCTAACTTTCCGTTGATCGATCGCTATTACAATTTTACAACATTGTCAATCAACTTTCCGCTGCACTGTGGTTGTTATTGGCCAATCCATTCGATTTCCTTCCGCATGCGGCGGCCGACAACGTTGACCTTTTTAGTCAACAAGGTCCGAGCGGAATACGTACATACATATGAACATACATATGTGTATAAACTACGAAACATAAAACAATCGTATGTACATAAATACATGTACATACGCCCCAGA

CGCAGGCCGAAGACAAAAAATCAAAGAGTCACGCAACTAAAAGCAAGAAATTAACTAAGCAATGAGTGCATCATTTTATTTTTGGAACTCAAATCAAATCAATAGCAATAGCAAGGCAATGCGGTCGGTCAGAAAAAATCAGCTAGCCTCGACTGGTTTTACAATTTCACCTCATCGCTCAAATTTGCAAGCGATATTTAATCGTTTTGTGATTAGAGGCGGGAACAATGCTTTGCAATCGACCGATTATTTCGATGATTTTGCATATGAATTCGTTGAGTTCAATTGGTTAAAAACCCCAAATACAACTTCTTAGGTCAAATTACCGTGGATATATTAATAAATTATTATTATTATACACATATACAAATCATGATTAACGTACCAACAAGATTGTCTTACGCATGCCTATAAAATTGTGCTTGTGCACTGATTTCTTGATGTCCTTTGTCTGTAATAACCGGATTCCAATGACCCATAATATTTAAGAAAATCGATAGTTGGCCAGGGCCTCTCACATAAATACAGAGCAAGGTCTTAACTTTTTAAGAACTTGCGGATGTGTCTGTTGGTTAAACATAACAAATTTTTTAGCTCTGTGATTCAATGTGGCGATGCCCTTGCTCCTTATGTGTCCAAACCTATTTTATAGCTGGCTTCTCTTCTGCGTCTGCCATATACTCAATGAATTGCTTAATGGTTTTTAATTTTTGAAATTTTATCTTGCAGTGCGCAGCTATGACAACCACGCCAAATGCGGTCTGGTATGGGTACCGCATGTGGTGGCCTACAGGTGCCGCACTTGCGGCATATCGCCCTGCATGTCCATATGCCGGGACTGCTTCAAGAAGGGCAACCACACGAACCACGACTTCAATATGTTCCTGTCACAGGCGGGCGGTGCCTGCGATTGTGGTGACACCTCGGTC

 

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