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CRIMIC CR01646

Gene CG31612

CG31612

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:21767209..21767231	40A2	FBti0210465	86478

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTCTGCCAGTGATGCTTGGTGCTTAT	CGCTCTTTCCCACATAGCTGCAGTTA

Images

Construct Design Sequences

sgRNA sequence	CCAGCAAAACGGACTCGGGGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGACGAGTTTCATGCCACGT	TTGCTGGTCCCATTATCCCT	GCCCCAACATAATGCTGAACG	ACCCCATGGCTTGACTAACG

 

Amplicon 5'

Amplicon 3'

AGACGAGTTTCATGCCACGTCCACGATTGTCCAGCCGCAGCCAAGTCAAAAGGATCCGCAAAACAAAAATGCAAACTTTCGTAGGCAAAAGTTTCTGCACATCAATTACGTCTAATTAATCGAAACAGCTGAGGGTAGTCCTGGTTCCTAGTTAGCGCTTCGAAATGGAGCAACATCAGGATTCAAATAAGCCGGCACAGAATTTTTGGGTGATTAAAGTGGCTGTAGTGGTTCCATTTTTAAAAAGCTGTGCTTTAAAGTGCTTTAAAATCGCCACTTTTAGCTATTACTAGTATAAAAATATTAAAATGTATTTTTTCAACTGTGTTGCCTCCTCTCGGCATGCATCGTAGTAATTTGCTTAGATCATTTCGTATTCAAGGAGGAAACAGATTTCTCTTAATAACGCGCGACATTAACAAAGTTTCAATCTTTAATCTAAGTTCTCGTTTCGAGACAAACCCGAGCAACGGATTTGGGTGTATGCGGCATTCTCTTGAAGGGGGAGAGACAACATTGTCATGCATTTGTAATGATGCCATCGCGAGTTTGAAAGGAAGGGAAAGACAGGGGCGTCGTTGATATGTGAAATGTCACTTTGTATGGAGTTTGAATAGCAACGACAATGACAACGACTCTGGCACAACAGCATGGCAAGGAGGCAGAGCTGTGGAGCTCGGTTGTGGAGTTGGAGGGGATGTCAGATGGTGGGAGGCCGCTACTCCTTGAAGGTTGTGCCAGACTTGACAAAAGGATGCCATTTCACCTGCACGGTTAGCCGTAAATTGGTCTTGTTTCTGTTGCTACCGCTGCAGGGATAATGGGACCAGCAA

GCCCCAACATAATGCTGAACGAACAAAATTATCTTTTGCTGGGTTCGTTTTCCCAGCTTCCATTTGTATTTTCACCTCCGACATTAACTTTTATTTCCTCAAATGCATTTTCTTCTCGGGAAGTTCTTGAGTTTCCCTAGCCATAACCGTCTGTCTCGTTAATACGTGTGTCCGCCCACAGAAAAACAGAAGAAATGTTCTCGGGTCCAGACCGCTCAACATTACGGTTTCAACTGGATTGCAGCATTTGGATTAGCTCATTGTTCTCGAAACAATTCAATTATAGCTTACTCCACATATCAGGCTACCATCGGAGCGAAAAACATATAAATCGTTAGATACTCGTATATTACATATGTAATCTAAGTTTCTAAATATTTCCCCCGTCCATCCAATGAAACCTTAACTCAAGCAGTCCCTTAAAATAGTTTACACATTAAATGCCTGCAGTTTGGCGTGCACATATAATTCGCATATATTCCCAAGCCAGCTTCGTTGCCAGGCTTAATTTCCAAAAGCTGTTCAAAGGCGAATCTCCCTGAGATAGGACGCCATATCCTGCACCTTCGTTGCCGATTAGTTGACGAGAACGTCTTGCGATGAAAACTTTGCCCTTTTCAACGCTCCTTTCCAGGCGTGGCACGGGCTAGGGTGGAGCTGTCATCAACACCCCCGCCATGTGCCGAGGAGGAGCGCAGCATCTGCAAGCTAGGACAAAGCGTTAGCTCTGTCCGGGTGTTGTGTCAACTTTTGCACGCCATCCATTAGAGAGATGCTCATGCCCTCAGCGGATGCTCCTCTCGTCGCCAACGAAATCGCTGAGCCAACATGTCACGGGTGCGGCAGGTGCGAAATGTATCCTGCTCCTCATTGTCGGCTGCACGAAGATGCAGGATGTTCGGGGCTGGCAGGTGGAGTGCGGTGGAGCCACAGCTCCAAGAAGGACTCCTGTCTTTGGCTGTCTTAGACAATCGCATGTGTAGTTTGGCGCAGCTGAATGTTTGCCCAGGCGCGCTCGCATTGTTGTTCCCCAAACTCGCATAAATATTTATGACAGCGTCTTTCTTGTCTTGTCTTGTCTTTTGGGCGAAAACTTCGGGGCCGCGGGGCGTTAGTCAAGCCATGGGGT

 

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