CR01647

CRIMIC CR01647

Gene bowl

CG10021

Information

Donor Location Cytology FBti Stock number
pM37-p22L:3784121..3771700 24C3-24C4Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK TCCTGTGCCTTGCCAAACCAAGATCA CGCATTCCGTGCATTTGAAGGGTTTC

Images

Construct Design Sequences

sgRNA sequence CGGGTAATTGCCATCAGGCCTGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
GCTCATCCAGCGAGATCTCC CAGACACTGCCCCCTTTTGT TGTGCTCAAAGTGTGAACGAA TGCAATTGGTAGCCCAGGTT
 
Amplicon 5' Amplicon 3'
GCTCATCCAGCGAGATCTCCGGCGGAGGAGGCGGTGCGATACCCATGCTGCGACCTTCCCGGATGGATCAGTTCATGAACTCGATGGCGGCGGCAGCGGCGGCGGTGGGTGGTGGCGGTCTTCCTGGTGCGGCGGATAGGAACGGCGGATCCGGAGGATCGGATGGTGGCTCCCAGAATGGCAACGGCGATTCGCGCAACTCATCGGCCAGCCGGATCTCAGCGTATGAAACACAGCTGGCCTATCAGCAGCATTTGGCCGGACTGCATGGTCCGCCGCCACCGCCACCACCATCGCATCATCGCGAGATCTCTGCCTTTGTGCCGGTTCTGCCCACCGGAAAGGTGCGCCCGGGTAGTAATTCCAATTACGAAATCATTGCCATGATGGCGGACAAGCGAAAGGAGTTGGCCCTAAGGGAGGCGGCTGCGGCGGCGGCAATGTTAGGACGAGGTCCTGGAGGACCCGGCGGTCCTGGTGTGCCACCGCCTGGAGTGTTGTACGGACCAGCTGGCGTGCCACCTCCGCCATATCTCACCGGACCGGGTCCATCGCCCACGGGAGCCGGAAGTTTTCCATTTCCACCAGGAGCCGCCGCCGCAGCTCTATTTCCACCTGGCCTGGGTCCTGGAATGCATGCCGGACTCGATAGGCGCCTGCTACGTGCTCCTGGTCGGGCATCGCGGCCTAAAAAACAGTTCATCTGCAAGTTCTGCAATCGACAATTCACCAAGTCGTACAATCTGCTCATCCACGAGAGGACACACACGGACGAGCGGCCCTATTCCTGTGATATCTGCGGCAAGGCGTTCCGGCGACAGGATCACCTGAGGGATCACAGGTGAGTCGAGGTTGGGGTACAAGTAATCACAAAAGGGGGCAGTGTCTG TGTGCTCAAAGTGTGAACGAATGGCGAATAATGGAATACCCTGTAAAGGCAAAGGATAATTAGATCAATGTAATTTATTGCTTACTAGGCAGTTTATACACACATTCTGAACAGGTGTGACAGTAATCTCGGCCAAAAGGGAAAGGCGACCACTACTCGTAGATGGAGTTTTATTGATTTTAGATATATCAGACAATCGCTTCAAAAGACCCCTTTTACCATAAGGAATTTTCCAGGAAATGTTTAAACTTTACGACGAATTTTCCTACCTCAACTCAAATAAGTAAGTCCCCATAAAGCGGACCCAAAATCAGAGCTCCTCTGACGGCCAGTAAAACTTCAACTCGGCCGCAGAGTACAACACAAAGTTAATTAACTCTGTTGGTTTTCCTTTGGGGCTCCTCCACCAGCATTAAAATTGAGGGATGGCCAAGGGGTTGGGGGCTATATATCGGGTCTGTGTATCCCGGTGCCGATGCCCATGCCGATGCCAGGCAGCAGGTCCAGCATCCCGGCTTCAATGTGTCGGCCTGTCCGAGCGTTCTGTCTTTTGATCGTTCGGTATCGGTCCATTTGTCGTTTGTTTGCTTTGCCGCGGGGCTAATTCTTCTTAAGTTTTTTGTATTTTTTTTTTACCAGAAACGCACGGAGCATGGGAGAAGAATCTCGGTGCTTCGGAGGCAGAACCTGTTTTGGGTTTTTATACATTTATTTTTTTTTTTCCACCAGCAGCTGCTGCCTCCGATGCGATGCTGAATCTGTGGCTCCGTCGAAGATGGGGACTTTAACTTTTAACTGTGGCTGCTGCTATCCAACAGCAAGTGGACGCCATTGCCAAGATGGCCGCCATAAACATAAAAAATTAATGCAGCCAGATCGCATACACGAAAGTTAAATACCCTATCAGTTAAGAGGATACAAAATCATAGGATTTCTTTAATGAAAAACTTTAAAGAATTGGGTTTAAGGTTAAAACAAATCCAGGTTAGGTTTCATTTTGAAAGAGAAAGATAGGTATTATATGCTATAGTATTATATTATTTATTCTATTGTCTGCTCTAGCCAGTTATATCCTTAGTTAGAGTATTGTAAAGCTGTATATCAGTGAGCTTTATCGGTTCAAGTTAAGGCTGCATTTGAGCAGAGATAACTGTCAACCTGGGCTACCAATTGCA
 
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