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CRIMIC CR01648

Gene zfh2

CG1449

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	4:524125..524129	102C4	FBti0210466	86479

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAACCTGCGAACTCCTTGACATCCAG	TTCTGAAGCACTGCCATGTTGTGGGT

Images

Construct Design Sequences

sgRNA sequence	ACAGTTGTTCGCCTCGACCCAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCAATCATAGGCCTTGCTGC	CCCAGGCGACACCATTAAAT	AGGCGAACAACTGTCGGTTC	CTCCAGTTACGGAAGGCGTT

 

Amplicon 5'

Amplicon 3'

GCAATCATAGGCCTTGCTGCAGGCATTTAATGGGTGATTGGTGAAGGGTTGTCTTTGAGGACCGCACATACCACGTGAAGGGTGTGATTATTGATCTTTTGAAAGGGGTACGTTCATTAATAATGCCGTAATATGCGGAATTATTAAGGATATTTGAACTTGTGACTGGATCTGAAAACTTTTTGCCACTGTTGAACAATAAGTCATTAGGCCAACAGCAGTAACGTCATGGGTTGATTAGTTGCGCCCGCGGGACATGCTTACAGGCTGTTCATTAAAACGCAAATGCCGATTGGGTTGATTATAAAAAGCCCTTCGGTTAGTTGCATCCCACTAATTGCTTCACACTTGAATAATCACGTTTGCTTCCAAATCCCAACCGGCATTGACAAGAAGCACCAATGAAAATATTCTTTGGTGAATTTGTAGAGAGGAGAGTGTTAAATGCCCTACCAACAGGTGCACTAGTTTTTTTCATTCTTACAATTTAATGTTGTTGGAAATTTGTATCACGCGGAACATTTAGGTTGCTTCTTTATTAAAAGGGTAAATATTATATTTACAAATGCGAACAGAAAGCAAACAAGATTATAAGAATATACCATCATAAATTGTTGAATGGAGCAGAGTACATTGTATATTACGGATCTATTCGAGGAATCTGTTGTTGTTAATAAGGTCCACACCACTTTTAGTCTCCGGTCCCATAAATATATATTATATGCCTGTATATTTCAATTAAGAGTTTGTTGGGAAATCTTTGTTATGTTGATATGATCTGCACGGAGGGGCAAAGTTAAATATTGCAGTGGCGTAAGTTTATGATGGCGGCTGAACCAACAAAAATTAAACCAATTGCGCTGATTTCACTTAATAAATGTTTCTGAACGCCTGTCCGTCCGGACCCCAATTGTTACAATTTAATGGTGTCGCCTGGG

AGGCGAACAACTGTCGGTTCGGTATCACTATAATTTTTCTGTCACTTTGTTAGGTACCACCAATGATACTACTACATAGTAAACTACTAATCGAATACAATTAAATCAAATCGCTTGGCCATTCTGAACTATATTTTAAATTACATATTCTGTTTCGTTGTTTAATGATTATTATATTACTATCCATATTTTCGATCCTCAGCTGTGAAATGCTTCTAAACTCGGCTCGATTAAATAGCGGCGTGCAAATGTCTACTCGCAATTCCTGCAAGACTCTTAAATGTCCCCAATGCAATTGGCACTATAAATATCAGGAAACATTGGAGATCCACATGAGGGAGAAACATCCAGATGGGGAGAGTGCCTGTGGTTATTGCCTTGCAGGTACTAAGTTCATTAAGCTTACAAAAATACTACTTAGTCTTTTAATATACAAAATTCAAGAAAAATTTATTTTTCATAATTTTACTACATATAATTTAGGGTTATTGCAGCAAATAGAACATATAAAACCGGTATATTTTATTTTTTTTCGATTGCTCATTGTTCTATTGCTCGGTTGTTTATTGAACACAATATGGTGTAGTTTTTATACCCTTAAAGTGGGGCACATCTGTCTTTTTTTGCTCGTCCACTACGAAATGGCGTTACATTGTTGTTTTTATTACTTTTTATAATGTGAACAGTGGTTTAGTCCTGTAATAAAACGATCTTAGATTTTTAAAAGAGTTTAAATCAGTTACCAATTTATTTAAACCCTTTTCCTATGGCACAGGACAACAGCATCCTCGGCTGGCACGGGGGGAATCCTACTCTTGCGGTTATAAACCGTATCGCTGTGAAATCTGTAACTACTCCACGACCACCAAGGGTAATCTTTCCATTCATATGCAATCGGACAAACATCTGAACAACATGCAGGAGCTAAACAGCTCTCAAAATATGGTTGCAGCTGCGGCTGCTGCTGCGGTGACTGGCAAACTGCTGCTCTCCAGCTCCTCGCCCCAAGTAACTGCTGCATGTCCATCCAACAGCGGGTCTGGCGCAGGCAGTGGCTCATCCAACATTGTCGGCGGCACCGCATCCCTGAGCGGAAACGCAACGCCTTCCGTAACTGGAG

 

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