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CRIMIC CR01649

Gene Syb

CG12210

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:10251780..10251795	46F8	FBti0210467	86480

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGTTTCCCTCGTCGTCAGTTCATCGA	TAGGAGCCAGTACACGTACACAAACG

Images

Construct Design Sequences

sgRNA sequence	GGGCAGCAGCATGACGTTTACGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTAGCTGCAAGCCACATGGA	ACGTCATGCTGCTGCCCA	TGTCCGAAAGTGAAGTCCGA	ATTTTGGCTTGCGGCACTG

 

Amplicon 5'

Amplicon 3'

TTAGCTGCAAGCCACATGGACAAGTTGTTAGGACCGAAACCACAGGTTGAGCAAGATTAGGGCGTTTGCAGGGCGTCGACTTGCGTTATAGATATAACAATACCAAACCAAACCAGCCACCTAGCTACACTACTGATCTTTAGCCTGATCGAGAAAAACCGCAACCAGAAAGAAGCACCAGCAAGAGGCAAAAAGCCGAAGCGGTATAAAATCGGTTAGCTTACCCAGAACGATGATGAGCAGCACAACGGCTATCACGCCCAGAATGATCATCATCTTCATGTTGGCCCACCATTGCTTGCGCTTCAGCTTGCCGGCCTGCTGCTCGAACTGGGATGCTCCCTGCTCCAGCTGATCCGCACGCTCGCCCAGTTCCGATAGCTTCTGGTCCCGCTCCAGGACCTTCTCCACGTTCACACGCATAATCCCGACCACCTCGTCCACCTTGGCTTGGGTCTGCTGCAGCTTCTTCTGGGCTGCATTGTTGTTCCTGATTTGATGGTCACCGAATTGATTGTAGTTGTCATTCGCGTTCGGCGGTGGCGGAAGTATGGGAAAACTGTACAATATTATGTGTGTTTGTGGGGGTTGCTTACACTTATTAAGAGCAGCAGATGGATCATCAAGCTGCAACGCACAAAAGCACGAGTCTTGGTATATCATGCCATCGTTATCTATGCCAATTCACCTTTCACGTTTTAATAATGGGACTTGTGTCCTATAAATACTTTCTTTCTTCTTAAATTCGGATAGACTAATAAAACTTCACAATCTCAAGTGCTGTTGAAATTCCATTGACAAAAAAAAATATAAAATGAGTAATATTCATTTGTTTTTCATAGATGTTAAATGAAACATACTTAAGTATGTTCAGTTACCATTTGCGATAAAGTGATTAATAAGCAAATTCTGTTTCTGATTATAAGCTACTTGCAAATGTTTTACAATTTAGAATCGAATATAATTAATACTTTTTTGTTATTCGAGTTAATTTGCCATTTCACATTAATATTCCATCTAAGATAGCAACTGACTTTACAATATCTCAACTTAACTGAATGCTTTTAATGACCCTAATTGATTTCAAACTACAACTGAAGCTACCGCTTGGGCAGCAGCATGACGT

TGTCCGAAAGTGAAGTCCGAAGACTTTACTTCCGGTTCGCTGTGGCCCATATGACTCAGGTGACGTCACTGTATGGGATTTTCCTTGCTTTTGTGCGCTGAGCCTGTTGATTCGATGGATTTCCACCACTTACTCGTTGTTGTTGGATCCCGAGGGGGAGGGGGCTTCGTTGTTCTCCATTGTGAATTTGCTGCGGAAAGAAAGCGAAGCAGAGAAAGGCCTTTATTGGATGTCTGGTTCTTTAAATGGGGAGAATGCATGTTCCATCACACACACACACCCGAAATTGTTTAGATTACAGGGCCGAAAAGAAAAGAAAACAAAACGCAGCACACGCCTTATCTATTCGGCCTATGAATCACTCGATTTATAGGAATGCCATCACCTTTGCTCGAAAAAAGAACTTGTTGCATGCCGCACTGCCAACAAACGCTGTTTACATGGGTGGAACGCCACCCCCAACCACCCACCACCCCCGAGCACTCACCTTGACAATGTGCCTGTAGAATACTGCTGTGTATTTCGGGTTTTCCACGGAAAACTGCGGCTGCTTTTTAAATAGCACTATTTTTTTTTATAGAAATATCAGCTTGTGATCAGTGTGACCGTTGAACTATTAGAGCTGGAACCAAGCCCGATACCTTATCGATACTATCGATGTTTCCTGAAAACCCCTTGCAAATATAAAAAGATAATACTTATCTAGCTTATAAAAAACATGAATTGGTATATTTAGTATGTGTATCGGCGCACAAATTATTGTTAACAAGCACTTGCATTCCATTTTTCTTTAGTTCCTTGCAGATGATGTTTGATTTCAAATCAATGTCTTATATTATTGTATCTTATTTTATTACTTAAATTACCAGTGCCGCAAGCCAAAAT

 

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