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CRIMIC CR01653

Gene fs(1)h

CG2252

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:8053556..8053571	7D5	FBti0210470	86483

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACCGTCCATACCACCGATTAGCACAA	TTTCTTCGCCTTCTTTTTGGCGGAGG

Images

Construct Design Sequences

sgRNA sequence	AACTTAACGCCTGAAATCGCAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCTTACGGATCTCCTCCTGC	TTAAGTTCCATTGGCATGATCTG	GGTCAATTTAAATCCCCTTCGC	CTTCTGAATGCGGGCCAAAC

 

Amplicon 5'

Amplicon 3'

GCTTACGGATCTCCTCCTGCAGACCGAGCAGCTTGGACTCGAGCATCTTCAGCCTAGCGCTCCGCTCCTCATCCGAGTTGGATTCGTTTTCGGTATCGCTGGAGTCCTCGCTGGACGAATCGCTGGCATCGTGCTTGAGTGAGGAGGAGCCGCCGTATCCGTGACCATGACCATGTCCGTGACCATGCCCGTGACCGTGGCCATGGCCATGACCATGCCCGTGGCCATGTCCGTGATGGTGGGCCGCATTGGCTACCGGCTCATCGGGGATGTTGGCATAGCGCATCTCAAATACGTCTTGCAGCTTGCGACCCATGGCCACAACATCATGATCTGGCGGATTGTACTTGTAGCAGTTGGTGAATATTAATCGCACGTCGGCGGCAAATTCCGGCGCGCTCTTGTACTCGCGATTGTCCATTTTCCGCTTGACTGTGCCCAGATCCATTGGTTTCTTGATGATGTCGTGGTAGTCATGCAGGCCAAGCATTTCCGCGTCCACTGGCTTGTAGAATGGCCAAGCATAGCCCGAGTGCTTCTTCGAGAAGAGCTCCTTGAGGATTTCGTTGCACGACTTGAGCGCATCCGACAGCTTCTCCTTGTTCTTGGCCACCGCAACACCAGCCACGCCGCCGGCAACTAGACCGCCAAGTCCGGGCACTCCGGAGACGCCTAGCGGCGACATATTGTATCCCGAGCCCTGGAATGTAAGATCCTGTGGTTCAGAATGACCCATCGTTTCGGTTTTTGATGATTTGGTTGATTTGTGGTTTGTTGTTGTTGGTGTTGTTGATTGATTGGTGTGGTTGTTGGTGTTGATTTGATCGATCGATTGATTGGTTGGTTGGTTGAGTGCAAACAAAATCAACGCAAAAGAAAAAGAAAATAAAACGAGCAAAAAGCAGCATGGGGTTAGCATCGAAATAAATCAAATGATTCCAAAACAAATGATTGCAGATCATGCCAATGGAACTTAA

GGTCAATTTAAATCCCCTTCGCATGTCATTGTGACAAAATGATTACCGATCATGAGGATGAAATTCGACTCTATATAATTACATATATGTTGTAGGTCAAATCAAATCCCCACTCACGTATTTCGATTTATTTACAAATCTATGAGATGGCTTTCGAGTAAAAGGCAGGACGGCGTATAGTGTATGATTTGATCGATTGTTGTTGTGCATTTGGATTGGTTATTATTGTAATTGCAAAGGAAATTATTCAAATGATCAGTCTGACCGAGATATGAGCTTGATACATCAAAAGTAATGCAAAACAGGAGCTTTAATTGAATGATAATTTTAATTAAACTAAGAAGAGTCGGTGTGACAATTACGACTTTAAAACTAAATACAAATTCACGCTTTTGAATCAATCTAGTTGATCTAATAGATTGGCACACTTTGTGTGAACTCCAAAAAAAAATACACTCCTGAATATAAACTTATATGTGCGAGCTCTTGAGGGACTATGATCATACTTATACGACTGGCGATGCCCGTTACTCTTACCTTCTTGCCAATTACCTGACGATTCGACTCCCGCCGCGTCGCAATCTTGGCCGATTTGGAGTCCATTTGCGTGTACGGGGATTCAAAGGCATTGGCCGTCGGCGTTGTTGTGTCCGCCTTCCGTTTGACACCCTTCTTTATTTTGGCGGGCTGCTGAGGCGGTATCACGGCGTCCACTCCGACGCTCACGCCCGCATTCACATAGGCCTGAACGGCGTTAGCGGCATTGACGGCAACTGCTGGTATTGTCCCTGCTCCTCCAGCGGCTCCCGCTGCACCTCCTGCCGCCGCCGCCGCCGCCGCAGCCGCTGCTGCTGCCGCCGCTACTGCGGCTGTACTGCCATCTAGAAGCGAGGAGCTATTGACCGCCGTCTGGCCGGGCGCACCGGGATAGGCGCCCGTTTGGCCCGCATTCAGAAG

 

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