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CRIMIC CR01658

Gene tty

CG1693

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	X:21328613..21339676	19F4-19F5		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	short band	GATCTGTGCATCTCACCAGCGGATTT	TTGCTGCTGATGGTGTTGCTGCTGTT

Images

Construct Design Sequences

sgRNA sequence	TCCCAGCTCAATTTCGTCTTCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGCAGTGGTATAGGTGCCAT	ACGAAATTGAGCTGGGAATCA	AACACACATATGGACACTGAGA	TGCTCTTCAAATCCTCGGGG

 

Amplicon 5'

Amplicon 3'

CGCAGTGGTATAGGTGCCATATTGCTGCGCCTGCTGTTGGTGGTAGATCGCATGCTGGTGCTGCACTGCAGCGGCCACGGCGGCAGCCGAGTGCGAATGCGGATGCTGGTGCGGATGGGGATGCGGATGCTGGTGGGGATGATGGCAGTAGATGGGCTGTGGTCCTCCCAGCTGTTGCTGTGCCTGCTGCTGTTGCTGTTGCGCCTGCTGCTGCTGTTGCTGTTGGGCGGCCTGCGTGGTCGGCGGCATGTTGGCGGCGGCGTTTGCTGCTGCTGCGACTGCTGCTGCTGCGGGGGGTGGCGACGGTTTTCAGTAAAAGTCATTCGCTTCCAATGTCTTGCATTGTTTGCTTAGGGTTGCATATTTACCATTATTGGGACCTATAAAAATGCACACACACAACGATTAGCAATAGGCGCGGGTTGGACGAAGGATGTGGATGTGGACGTTGGATGCGGATACAGGATGCAGGGTGCAGGTGGCGACGATGGTGTGGTCATGGAGCGGGTCGATGGATGGTCGCGGGAATTATGCATGGAATGAGATTATATAGAAGAATAAACGTGATGAGAGCGAATTGAGCGGTTTGCCGTTGCTTTACAAATATCTAAAACGGTTGCGAATCGGTTCAGTATCGGTTATTTACTTTCAAAGTTCGACCAAATAGGATATGAACCGATTTTAACGCTTATTGTTTATTTATTCACAACATTGTATGAATATTATAAATAAAAAGTAAGCAATTTTACTTTTTTTCTTTACATATCATACATTTTACATATTTTACTTTTTTACAACACAAACTAAGTTCATTTAAGATTTTACTAAATTGCGCAACATTTTTCTAATTGATTCCCAGCTCAATTTCGT

AACACACATATGGACACTGAGATTGATATAAATATGTACATGTACACTGTGATAAAAAGCGCCTCAATGAAAGCTATTAAAAATATCAGAATAACAAATACTAATACTTTTAAAATTCATACGCAATAAACCAAATGTTTCTAATGAAATGAAGCTCTCATTCTTTGAGTAAAATCTTTGAAATCTTTTCTGGCTACTTTGAATTCAATTTAGTTTATTTGTGCCTCTTCAGTTGGGCGCTTTTTTCCTCGGTGTAGTCAGATAGAGTAGTGGGCGTGTGTATCGAGTTTCGGGAGTGGGTGGTGCGGGCTCCCCACTTACCGGTCATGTGGGTGGGGCTGTGGTTATGCGATGGCAGCCGGCCCAGCGTGTGGGTGCCCATGGCGCGCATGTTCTGCTGGATGTGGGCGTGGGCCATCATCTCGTGCTGCTGGCGCTTGCTGGGATGCTGGAGCGTGTGGGAGCCGCTGATCACCGGCGGACTGAAGTGCCTGGAATGTCAGATATGGCGCAAGAGCAGAGAAAACGGGACGAGAGAGAACAGAGAAAGAGAGATCGAGAGAATGGAGGCGGGAATCGGGCGGGACGGGTCACGAAGGGATCTTCTAAAACGTACCCATTATGATTTCGCGGCAATGTCCTGGCGGCGTTCATCATCTGCTGGTGGTTCTGCGGTGCCGGATGCGAAATATACGACGGCTCGTCCACCTGGGCATAGTCATTCCGTTTCCGTATATAAATCCACGTGTGCGAGTCCACCCACACCATGATGGTTAGCAGAATGGCGGCAATGAAGCTGGCTATTAGCATCAGTACCAGTCCCAATAATCCTCCCTCGCAAAGTCCTCGGGCGGCGGCCAAAAAATTGTGATGCACTGCCTTGCAATCCACGAGCGCCGTCAGCTGGGTCAGCAATCTCTCACTGCTATTCAGATCTGCATTTACAGCACCCAGCTTGGGCTGCAGCCCCGAGGATTTGAAGAGCA

 

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