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CRIMIC CR01659

Gene Usf

CG17592

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	X:4548794..4544617	4C7	FBti0212941	91269

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	ACACGGATACGATTCTGGCTGGGATT	CATACAAAAGCAGCTGTGTTGTGCCG

Images

Construct Design Sequences

sgRNA sequence	TTAGGAATAAAGTTACCCAAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCGGTTAGGCCAACAACTTG	AGGGCGGACACTTGATCTTT	TCCTAACCGTATGCGATCGA	AGCAATGCGAATCCAACAGC

 

Amplicon 5'

Amplicon 3'

GCGGTTAGGCCAACAACTTGGCTTGAGTCACCCAAGTTCGACTGCATATATGTACATGCATATACATAGCCATATACATAACCATATACACATATCGATACACATATGTAATATATGCTGCTTTTCTTATATAACATATAACAGAGCGGCGATAACAAGGAATCACTCGTCATGTCCGTTTATTGTACTGCGTGTGTTGCGTTGCGTGTACGTTGCACGTCATTCTTTGGTGGATGGGCGTATATGTTTGTTGAAGGCGCCAGGGCTTGTCCATCCGCCGCCTCCATATTTGTTTATTCCCGTAACCAGCAACCCAATCGGTCCACCCATATGTTTTTCATTTCTATTTTTTTTTTTTTTTTTTTTGTCTATCCACTCCTATTTCGCAGGCAGATTTTGTTTACTCATCAAAATCGCTGATCAGCAGGCCACGCTTGCCGAATCCCAGCGACGAGACGGCGGCCAGATTGGGTGTCGTATCGATTCCCTCGAATAGATCACTGGTCGCGGAACTGTTCAGCGAATTTAGGGTAACATTAAACGTCGATCTTCCGCCGGCCGTGTGGAAGCGTTCCTGGAGCTGCTGCTGACGCTTCAGGCGATCAAGTTCCTCGCGCAGCGCCTGGTTGCTGGCCCTTAGGCTATCCGTCTCCCTTAGACAGTCGCGCAGCCTGTTTATTGGTGGGGATAGACAAAGTTGAGTAATTGGTTTGTTTATGTTTGGTGATCGGTGAATCGGTGGATCAGGTGTTTGATTGGCTTTGTATGATTTTGTTGGGTTTTGTTTGGGTGGAAACCGCATTCGATCAGTGTGAGAGAGTGTTGTGTTCAAGACTTGAGTGACACAGGGACATTCGAGAGTCCAGCTCATATTGCACGAGATAGTGGCGAAAAAGAACCGCTCCAAAATGGGGATTTTGTTTTGCAAAAAGATAACACCAGCCCTATATCAGCACTTTAAGTACGCATTTCTACGGAAAAAGTGGGAAAGATCAAGTGTCCGCCCT

TCCTAACCGTATGCGATCGATACATGATGTAAGCTCAATTGAGCGAATGGAGTTGATTAAGATCTATAAGCAAACTCATTTTTTAAGACCATTAACAAGTCAAAAAAGTTATATAGCTGTATATATAACTGTAGTACAGCAACTTATAGCGGACAATATTTGTGGATTTCTGATTCAGTGGTTTCCCCCCTACAAACCCGTCTCCACTCACGTGTCAATCTCGCCCTGCATGCTCTTGATGTACTCGCATGCCTTGATCAGGATCTGCGATTTGGAGTCATTGGGCGGCGCTCGTCCGCTGGAACTGCTGGCATTGCCTTTGGAATGGCTACTTCCGTTGGTACTCGTACTTCCGCTGGTGCTGGGACTCGTGCTGGCCTCGCTGAAACTGGAGCTGGAGCTCAGACTGGGCAGCATCTCCTTGAGCTTGAAGATCCAGCTGTTGATCTTGTCGCGGCGCCGGCGTTCGACCTCGTTGTGCGTGGCACGACGTTTATCATCGCGTTTCTTGTACGCCTCCAGTTTGCTGGATGGTGTTTGGCCAGCTGATTCTGGCTGGGATCGGGCTTGGGCGTGAGCATGGCCATGGCCATGACCATTGCCATGGGAGCCATACAGGACACTGGTGGTGGTGGTGGTGCTAGTGGTGCTGGTGGTGGTGTTGATGTTACCTGGAATGGGAGGATTCACGGTCTGGGCGGTTATTACCGCGGACTGTGTGCTGGAGGAAGTTGTAACAGATGGAAGGAGCGGCTTTTCGCTTAGCGTATCTAGTTCCAGCTTGATGGCCGCCTTCGTCGGCGGCTCACCCATTAAGCTGCCGGTTTCCTTGACCAAATATATCTCACAGCCAGTGGGCAGCTGCAGATTGTTGGGCAGCTGCAAATTGCTAGGCAGCAGCGAATGGATATCTAGATCACATCCCGGTTCCTGTTTAATCGTATAGTACTCCTGCGAATCGTCCGAGTTCTCGTCTCCGCAAATGGTGAGAAATATCTGAGCACCTTGGCTATTCATCAGCGGATTTCCCGTGTCGCTGGTGAGAATCAGACTTTGCTGACCATTGGCAAAGGAACTGTGGACCAGGGCCTCTGACAAATCAGCGGTGTGGTGATTGGTAATGGTCATGAGGCCAGGTCCAGCTGTTGGATTCGCATTGCT

 

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