• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR01661

Gene Wnt10

CG4971

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:7365802..7365821	27F2	FBti0210471	86484

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGCAACGATCAAAACCACTGTGCCCA	ATCCAATCCGTTCGTACAGGGTGATC

Images

Construct Design Sequences

sgRNA sequence	TGTTCGCAGTTGCTGAACCACGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTATCTTGGCCTGCTGCACA	ACATTGCAGGGGATTCCGAT	GGGGGCCTACACACAATTGA	GAACGTGTGAAATCCGCTGC

 

Amplicon 5'

Amplicon 3'

TTATCTTGGCCTGCTGCACACGCTGGTGAGTCTCTCATCTATCCTAAGACCTAAAACGAATCCATCACCATTCGCGAGCTTGTAAAGCAAAACTCGTAGCTGTAAAATGCCTGTCGACATATTAACTCCCTTTCCAGTCCCGGGCTCATTACGCCGTGTTATTTGTGGCGGCCTGTTGGCGGGTTAATTAATTAAACGACAAGCGGCAACAGCGACAACAGATTAATCAATCGCCGCAGTGCGCCCGAGAAACAGCTGTCAGCCGAAGGTGTTGGCATAATTAATATCGATCTAGTGATAGGCCAGCGAAATTAAGCCAACCTAATTGTGCCCGATTAACTTAAGATTGTTCGCGGTTATGGGAATTACGAGGTGGAACGCAGACTGTTTTCACCTTCATGCATGGATTATTGTTTTGGTTTGGGAGAGCATTAAAAATAAGATGGGAACTAATTTAATTGTGAGATAAGAAGATGTTCAGAGGCAGACCTAAAGATCACCCAAAATATTATAAATAATAGCTCAGATACTAGTCATTTTTAAGTTTTATTACCCACACAGTGCTGCATTGTTTAAGCTAACCCATCATTCCTCGCAAGCAAACCTATTTGGCGGCCATGCCACAAAAATGGCAACTTACATGTTAGACCATTCGCAACAACGACCGCCATTTTGGAAGGCCGCAGCTTGCCACAAATGAAATTGCCAACGGGAGCCCGCGGTCGCCTGCTCCAACGGCAGTGGATGATTGGAGCCTGGGATTGCCGGCTGTCGGAGTAGGAATCGAAATCGAAATCGGAATCCCCTGCAATGT

GGGGGCCTACACACAATTGAGCTGATTCCAATTGCAGGGTGGTCCCCGGCACTCAAGCTTTATAATGCGCCTGGGTCGCTTGTCAATCCGAGTCTTCTGGCCAGGCTCTCGATTTTCCATGTGCGCAGTGAGGCGGAGCCGGGAACAGGGTGCATGGATCTGGGGGCAATGAGCAGGGAGCAGGGAGCAGGGATCAGGGAGCTGGGATGGATGGATGTTGGATGTTGGATACTGGATGTTGGGTGCATTACTGTGCCTTGCCTGACTGGTTGCCTCGCCTAGTAGTTGCAGCTTGTTAATCTCGTTACTCTCGTTGTTGTCATACACTTTCAAAAGGTACTTTGGTTAAATAGGTTTAAATCCCAACAACAAAAAATTAAGCCCAAATACTCCTTTTTATTTAAAGACTACCTTACCTTACGTCAATATACATACTTTATAACCCTTTAGTCAGCCATTCAATATGAATATTGAATAAATATTTGTTCTAAATATAAAACTAATTTGCAAGGGTGTTACCATCTTCGAATCCCAATGGGGCTTCAACCTTTTCCTGTTTCTTGGGGTTGTCAACATACTTTTTGCTCTGCTCGTTGAGACTGCAGCAGTGCAACGATGTTGCAACTGAAGTGCAGCCTGCAATTCGCTGGCGATGCTGCTGCTCTTCTCGGCGTTGCCTTCGTCATCCTTGTAGTTGCAACGTCATAATTGCAACGCATAATGAGGCGAACACACACCCACATCCAGAGCCATGGTGCGCCACAATTTCGCCCGTTTGAAATGCAAATGAATTGTCGAAGTGCCACGCCTCCGGGACGCCAGTGCTGCAGTTTGCAGCTTAATTGGGTGCATACACAACACACATCCACGCACCCACTGCACACCTGCGCACAGGTGAGGCTGCACTGCAGCGGATTTCACACGTTC

 

---

Search Again