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CRIMIC CR01662

Gene CG17597

CG17597

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:18829802..18829808	37B1	FBti0210472	86485

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTGCGTACATATGTGCAGCCCTGAAT	TACGAAGCGTTGACCAAGGCACTAGA

Images

Construct Design Sequences

sgRNA sequence	ATGCAATACCGAGTGGTTGACGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGCATCTCTCCCGCTTTGTT	CACTCGGTATTGCATGCAGC	CGGTCTACGGTCTACTTATTTCT	ATCCGTGATCCCGCAAAGAA

 

Amplicon 5'

Amplicon 3'

TGCATCTCTCCCGCTTTGTTTACTACTCCTTGGATGTCTACTCGGTCGTGGCCCTAAGCCTGCTACTACCTGTGATCACGCTCTTGGGACTAATCCGAATGTTTAAAAGAAGGGAACCCAAAGGTGACCGCAAACTAAAACGCAAATAAGCCCAATCAATAGATTACTGTTTTGGTTTTCAAGAATTACCTTTGGATTTAAACTATGAAACAATAGATTGCTAAGTCAAATACGTGTGATACATTATTAGAAACCAAAATACATTTTACCAGAACAGAATTGGCAAGAACCAGTTTAAGTTTTATATAAAGCAGTTCCCGTTTAATAAAAATATTTACAAAATCAAGATCCCATCCCTTACAATTTGGCCTTGGCCGCACCCGGGAAACCCAGACGATACAAGGCAACCACAACAGCACCACCCAGTCCCAGATTGTGCTGCAGAGCCAACTGAGCATTGGGCACCTGGCGCTTCTCAGCCAATCCACGGAGCTGCCAGCAGAGCTCAGCACATTGTGCCAGACCCGTGGCGCCCAGAGGATGGCCCTTCGAGATCAGACCACCACTGGGGTTGACCACGAACTTGCCACCGTAGGTGTTGTCTCCAGCGTCGATGAACTCGCCGGCCTTGCCCTCTCCACACAGTCCAAGTGCCTCATACGTGATCAACTCGTTGGCCGAGAAGCAATCGTGCAGTTCCACCACCTGGACATCCTGGGGCTTGTACCCACTCTTGGCGAACAGACGCTCGGTGGCCAGACGGGTCATATCGGTTCCAGCAATCTTCATCAAGCTCTTGTCGGCAAAGGTGGACGCCGGGTCACTGGCCATCTCCATGCCCACAATCTCCACAGCCTGCTTTTCCAATCCGTGGCGACGCACGAAGGCCTCGGAGGCGAGAATGGCAGCTCCAGATCCATCGGAAGTGGGACAGCACTGCAGCTTGGTCAGCACTCCCTCCACAACCTGGGGCGACTTCATAATCTGCTCCAGGGTGTATTCATCGCGGAACTGCGAGTAGCTAAAAGAAACAATGGAGAGAAATGAAGTGGCGTGAATGAGGATCAGCTGCATGCAATACCGAGTG

CGGTCTACGGTCTACTTATTTCTCTGTTTTCAGCGATAAGATAAGCACACGCGTAAGGTGAACTTTCGACTAAACTTACGGATTATTGACGGAGTGCTTGTGGTTCTTCCAAGCGATCTTGCCGAAATGCTCGGGCTTAGTGCCATACTTCTTCATGTGCTCCTTGCCGGCGTTGCCGAAGATCTGGGCAGCCATTGGACCAGCCCCGATCTCGGTCAGCTCACTCATCTCGGTGATGTGGCGCTCCATGGGATTTGCGCGATCAAAGTACTATTAGAAGAGCAAATAATGGATCAGTAATTGGATTTTTTAGAAGTGGACTCGTGTACCTTTGCGGACAATGATCCACGCTCCATCTTTTCGAATCCCAGAGCCAGGACGCATTCGGAGTTTCCGCTCTCTACGATCTGCTTGGCCAAATACAGTGCGCTGGATCCCGTGGAGCAGTTGTTGTTCACGTTATAGACCGGAATGCCGGTCATTCCGACCTCGTAGATGGCCCGCTGTCCGCAGGTGGAGTCTCCATAAACGTAGCCGGCCACCGCCTGCTGCACTTCCTCGAACTTGATTCCCGCATCCTGGAGAGCTTTGGTGATGGCTTCTTTGGCGAAGTCCGGGTAGCAAGCATCAGCTCGGCGACCGGGCTTCTCAAACTGCAGGAAATTGGAAGGAGATGGTCGGATAGATATTAACACCCAAGGCGGCACAAAGGTCAATCATTCGCAGACGAACCTTGGTCATGCCAACTCCGATAACGTAAACTCTGGTCTTGGTCATTTTTCGGCTGTTGGTTCTTTGCGGGATCACGGAT

 

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