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CRIMIC CR01663

Gene CG17564

CG17564

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:19299554..19299567	37D2	FBti0210473	86486

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGAACTCGTCTATCTCCTTGGTGACC	CAGCGAACCATCACACAAAGCACTTC

Images

Construct Design Sequences

sgRNA sequence	ACCCAATCCTAAAATGATCCTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTCGCCGTAGAAGGATTCGG	TCCTGGTAGTGCAGTTTGAGA	TGGGTACTTCTCTTTCAGCCT	CGTCGTACTCCCGGAGATTG

 

Amplicon 5'

Amplicon 3'

TTCGCCGTAGAAGGATTCGGTGAAGGTTGGTAATCGCATCTATTGTGCGCTCCTTGTCCATGTAGTTGTTGTTGATGGCGTCCTTGGTCTTGGCCAGGATCTTCTCCCATCTGAGGCACTGCACTCTGGACTCGTTGTAGGATCGTTCCAGTTTGGCCAGATCGTTCTTTAGACCCAGCACAGTTAGAGCCGCATCATTTGTGATCTGCACCATACGCTGCCGCATGTTCTCGATGTCCTGGAGCTTTTGATGCTCCAACTTGTTGATCTCCACTTGAGCCAGCACTGTAGAACAAAAAAAAATTAATAACTTTATTTCATTTTATAAATTAGCAATGCTCACTCAGCGCATCACAGCGATCGGTGCAGTCCTTTGGCGAGACGAATATGTCGGATACCTTCACCACCTCGTCGAGCACCTTCTCGTAGGGCTGGAACTCCTGCACGGTGGCCTTGAGAGCCTCCCGAAAGGCCTTGAGTTCGCCGATCGAGTCCTTGAAGCTCTCAATGCCCTCGCCGAGCTCCTTATGGTTGAGCGTCTCCGCTTGGGCGGTCTTCTCGGCCACTCTCTTTTTGTCCGCGCAGTCCTTGATGAAGCTGTTCACCTCGATGAAGCGCCGCCTCAGCCGCTCCTGGATCTTGTACATGTTCCTCACCCGGGCGGAGTTCAGCTTGGTCTGCCGCTTGGCACTGGTCAGCATCTCCTCCTGCAGCTTGAGCATGGCTTCGTGCTCGCGGAGATTGTTCACATATATCAGTTCGATGGAGTCGCCGGCAGAGTCCGAGTTCGGTGGAATTCTACAGGAAGTTACGAATAGGTCAAATAATTGATACCAGATAGAGACATCCTTGCAACAGTAAATTACGAAGCTGTAAAATTTCTCAAAAATCTCAAACTGCACTACCAGGA

TGGGTACTTCTCTTTCAGCCTTTTTTCAGTGTGGGATTCACTACTTACACGAAGAATTTGTTCTGCTGCTTAGAGCTGATATAGTCCCCCACGGCATCCTCCGGCCGCAGGTCCAGATTTCCCAGAACATCGAGCTTCTTGGTGGGCTTCACGCGGGGCATCTTGGATCTCTTTGCCGAAAGTAAAAGCTCAAGTGTACGTTTTTGTTAGTTTTTTATATTTTTTTTTGCTTTGGTTACCGCGATACAGCGCGGATTGTGCGTTTTGTAGTAGTAGTTGTGTGTGCTGTGTGCTTACTTTTCAAAGTGTGTTATTGTTTTGTAATAAAAATTAACAAGACCTACTTGGAACATGTTTTTTATTTGGGTGACACATAAAAACAATTGGTTGCCAGGAGGTTCTTTTATTACCGTGGCAATGGTTGAGAAGGTGTAACCAGTTTTGTGTCATGGTAACTGCACAATTTGGTTTCTTTCGAAAAAACAAAATAGGAAGCTTCTTCGGCAAGCCGAACCTAATATACTCTTGATACTCGATGATGATTAATTTTTAATCAACAACTATAGAATTATGATATCTTTTCAATTGTTTTTACTTGATTTAACGACTGGTCGTACTTATGAAGGAAACGTCAGCAATTGCATAAATATCTGAATGTAGTAATAAAAAATGGTTTAATCCTTTTATCCTTGACTGTGCTGTCTGCCCATATACATGTAGAATTACTCATCACACATCGTTCTCCAATAGATATTCCCCCAATATATATCAATATATTTGAAAAGATTATATTTTAATAGGACAAGATGCCGCGACGTAAGCCCACCATTAAGACCGATGTGATCGGGGATCTGGACCTCAGTCCAGAGGTCGCCATCGATGACTATCGCGTCTCTCGCCAGCAGGACCAATTCTTCGTGTAAGCTAAAAGAGCAACTACAATATGTAAGTCATACTATTAATGAAACTTGGATGGTCTAGGAAACCGCCCAATTGGGATTCGGCCGGGGATAACATCGAGATGCTGTACATAGCCAATCTCCGGGAGTACGACG

 

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