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CRIMIC CR01664

Gene CG15130

CG15130

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:20424034..20424037	38C6	FBti0210474	86487

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAACCAATTGGGTGTGACCAGCAGGT	GTCGAGTCATCGTCAAATCCGTGTCT

Images

Construct Design Sequences

sgRNA sequence	GGTAACTAGCGTGCGACAAGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCATCCCCGCAATTAGCCTG	CGCACGCTAGTTACCTCACA	AAGAGGAGTCAGCTGGAGGA	CACCGCACCCATTTTCTTGG

 

Amplicon 5'

Amplicon 3'

TCATCCCCGCAATTAGCCTGTATGTGTAGAAAGCGCATTTAAAAACGTACTGCAAATATTAACAAACCTTTGATACAGCAATCGGAATTAATAAAAACGAGAAAATCAAACTGCAATGTAAAATAAAAAAAGGGACACTCTTTTAATCTCTAGCTAGCTGCAGACGCTCGAATACACTTTATTTTCTAGCTTATTAGCTTTTAATTGCACATAATGTCTTAAATGTTACTAATATTTCGCTTAGGTATACCATCTCCGCTGCACAATACTATAACAGGCACTTAGATCTTAAACTTAACATTCTGCTTCGCGTTCATGCTCTCCATGCTGACCTGGAAGTTGAGATAGTCGCTCTGGATGCGGGCACACACTGAAAGAAATGTGTTGGGTAAACTAAGTTGGGACTGAGGAGCGCTTTGACTGTTACTATTGAGATGTGCTACTTACTTACAGTGGCAGTGTTCATTGCGATAGAGAGTTTACTGCTTAAGTACTTCCGATAGTTTGTACAGGGTGCGGGTGAATTTACAGAGCCCCTAGGGATCATCGTACTGTTCCAATTTGATATAAGCAAGTGCTGTGATAGAGAAGCGGCAGGGCGAGATGGATAGAATAAGTGAGTAAATCGCTTAACACTGGGAAAAATTCGTTCCAAGCATGCGAAGAGTTCTCATAAATTTGGAACAAGTCCAAAACTCAAGAAGATACTGACTTGAAATGCAATTTTGATTATTATTCTATTAGAAGAAGATAACTGAGAGTGAATATTTTGAATTTGAAATATTTTAAAATAACGTAACAATATTAAAAAACCATTTCAGTCGAAATTAAATTGATTACTGGTGGGATAACTGAAGATCAAAGTTTTGTATCGCAAGCTACTACTTTTCCGTTTATGTTATTTTAAGCCCCTTTTTATAAATTTGCTATCAAGATTGGAAAACAGAAGTGCTTTTCGTCCAATTGTGGTTCGGAACTTATCTTAAATGGTTGTAATGCCATAAAATACTACAATTAACAAGTTATTTAGACACCTAATTATTCAGCTGACTAGCATCTCTCTATAAGAAATCCCTTCACATTCAAAAACTTCAGAAAGAATTTTTCTCAGTGTGAGGTAACTAGCGTGCG

AAGAGGAGTCAGCTGGAGGAGTCGTTTATCATTTTGTATATATACAAATGCGTGAACTCGTGTGGGAGGTGGGTGGTGGATGGGCGGGATAGTGAGGATGGGTAGGAGGAGCTCCAGACAGGAAAAGGGCTTGGCTCGCATTAGGGTGACAGCGAAAGTGGGGCAGTTGGTACACAGGTTTTCTACGCGATGTGTATGTATGAGCGGGGGTAATGAGATGGCGGAGCCACTTTCTCACCCTTCTGCAGGTCCATGTCGTCCATTTCGGAGTTCTTGATCTGCTGCAGCTTCTCGCGGAACTTCTTTGTGATCTGCTTGCGCTGGTAGTCCGCCTCGATTTCCTGCTTGGTGCGCGGTATACGGAAGCGAATGCAGCAGCATACCATAATCACTGCGGCAATGATTCGATTAAACTGTGTCCAGACCGAAGCTACAGCTATGATGTACTCACTTATTAGGAGGAGGACACCCAGAACGGCGGCGATAATGTGCCACACTCGGAAGCCCGCTATGACCACACTTTCCAGCCACATTTCATCGAAGATATCCGAATCGTCATCCATTTTCCGCTTGGTTTGTGAGCTCTGTCAGGTGCTGGAACCGCGGAGCTGCTCCCAAAACACGTCCGGCTGCCTGGCCAATCCAGTTCCTACTGCTGGCCCAGGAACTGGTCCGAACTGGCCTGTCCCACCAAATAAGTTGGTAAGGGAGGCGCGGCCCAACACACTCCCTTCCTTCTGGAAAAATAATTCGCGGGGTGTATCCATGTGGCGGCAAAATGGCTAACTTGTAAAATAACAAACAATGGAGCCATCCCCTCCTGTGGACAAATGAAGCCTGAAACTAGAAAAGGCAATGATAGATATTTTACTAGACACAGGGCAGGATTGCTTCGGATGGAGGAATCTGGAACACCTTTGTCCTTACGAAACTTGCAATAGTTAGTCCTTAACAACTAACAAGTTCATGTTCTGAACATAAGGTATATTTGGAAAAAACGATAAAAACCTTTTTTAAAAACCTTCATTTGATTTTAAAGTGGTAAAATTATACAGTTCCTAAATATCTGTATAAAGTATTATGAACAAACTCAACCTCCTTTGTAAAAATCCGTCCCTGGTACATTGTGAATATGAATGCTAAAATGACAACAAACTCGACTCGAGTCCAAGAAAATGGGTGCGGTG

 

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