CR01666

CRIMIC CR01666

Gene Doc3

CG5093

Information

Donor Location Cytology FBti Stock number
pM37-p23L:9007249..900519666F1FBti0212942 91270

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
no band OK TATGGGTTCCGATCATGACGGTGGAA ATATGTCCGAGATGCTGAAGCTGCTG

Images

Construct Design Sequences

sgRNA sequence TAAGCAGAAGGCTTATCAATGGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
TGTTTTTCTCGTGCCTCCGA GATGGGGGCGAGCAAATAGT ACTCTTAAATCTTACGGCGCA GTGATGATGGTGCTGGGGAA
 
Amplicon 5' Amplicon 3'
TGTTTTTCTCGTGCCTCCGAAATTACAGCTTCCCATTTCTGGGACAATGTAATCTCGCCGAACATTATTGGTTGGCCCATATCCAAAACGTTTTCCAGTTCCATAAATATTACAAACGATTAGGCGACTTCAAAGAGCGCTCCCCCGATTCCACGAAGAAAAGCCCCATTCCCCATTGCCGTTCCCTTTCCACGTAACGCTATCAGCAGCAAGATGGGTGCCGTGCCCCCCAGTTCCGTGAGAGGAGGTCACCAGAACCGCTCCACGCGGCGTTACGAAAATGGCGTTCCATCTGGCGCAGCGAGTGCAAGCGGGACGGCGTAACGCTCGCTGATAAGCGACCAATGGCAAAGAGATAAACATACACACATGGCCTGACGCACTGGAAGTGGAAGCGAGAGAGCGGGTGAGTGGCAAGCCACTTGAGCATGAGAACAGGTTAGGCAACTACCTGAGGATAGGGATCGGATCGGATCGGATCGGCAGAGTGAAGAGCGAAGTGAGCGGGAGGAACCCAACCGACCGGATTCTGGCAGAACTGAAGTAGTGCCCGCTCTGAGATTCCGAGAGACAGTCTCAATCTGGACAGCAAAGCGATCAGTTCGTGTTCCAGACGTCGATTGCCCACGGAATAATATCAACGTATCACAAAAATAATAGATTACGCACATAGCTCCACGAAGACCCCAAATATGTTGACCTTGCCCAACATCGCCGATCTGCGATTGCAGCAGCAGATCGCCCAGGAGATATACCGCCAGCAGATTATGCAACGTTTACCCGGTAAGTGGAGCACTCCCTGCGCTTGTGATTTGCCGTCGGCTAACCCAAAGTGTCTACCACAGATCCCCTATGCGGCCTGCTACCCAACTTTGCCGGACACTTTATGCCACCGCCGCCACCGCCGCAACCCACTTTGCCCGGCGATGTGGAGGCCAAGCTGGAGAATAACGAACTCTGGCAGCAGTTCCACAGCATCGGCACCGAGATGATCATCACCAAGTGCGGCAGGTTGGTACCATTTTAAAGTCAACTATTTGCTCGCCCCCATC ACTCTTAAATCTTACGGCGCAAACGTGTCACTAACATCCATTAACCAACGCCTCCATCCCTTTCAGACGCATGTTCCCTTCGATGCGGGTTTCCCTCAGTGGACTGGAGGAGGAGGCCAGCTATTGCGTGCTCCTCGAGATGGTGCCCATTGGCGATTGTCGCTATAAGTTCTCTGGATCCCAGTGGGTGCCGGCCGGAGGAGCCGAGCCCCAGAGCCCGCAGCGCATGTATCTGCATCCGGAGAGTCCAGCCACGGGAAAACACTGGCAATCCCAGGCTCTGCTCTTCAGCAAAGTGAAATTGACGAATAATACGCTGGATAACAATGGTCATGTAAGTAAACTTTGTCAAGAATATTACATAAACAAATTTGATCTTTTGTTCTTGAGTAGAAATATATGTATCTTTGTATATCCTCAAACTAACCGTGTATTTTTCTGTCTCTCCAGATCGTCTTGGCCAGCATGCACAAGTATCAGCCGCGTCTGCACGTCATACGAACCTCTGATCTCGGCCAGATTCCCTGGGCCCCCCAACAGGCCTTTATCTTTCCGGAGACGGAGTTTATAGCCGTAACAGCTTATCAGGTGCGTGGTGCTATATCGCAGTCTAAATATGGCACGTGATAGCTAATCCTCTTGTTTGCATTTGCAGAATGACCGCATAACCAAGCTGAAGATCGACAACAATCCCTTTGCCAAGGGATTCCGTGAGTCGGGTCAGTCGAGATGCAAACGGAAGATCAATGACTCGCCACCGCCAACGCAGCCCGAGGCAAACCAGGTGGATCGAAGCCCGGTGACATCGCCGTTAGCCAAACGGTTGCGACTCGTGGAGGAATTTCCCCAGCACCATCATCAC
 
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