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CRIMIC CR01668

Gene CG7497

CG7497

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3L:17635476..17635497	74E3	FBti0210475	86488

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGCCACTGTTTGTGTCCAGTTCATGG	GTTGATGCTCTTGCGAATGAGCTCGT

Images

Construct Design Sequences

sgRNA sequence	TTGCGATGCCTAACGATGCACGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGTGTAACTGAAGACGGCGA	TCGTTAGGCATCGCAATGAC	TCCCCGGGAATTGTCAATCC	CATCCAGAACTGAGCCCGAA

 

Amplicon 5'

Amplicon 3'

GGTGTAACTGAAGACGGCGATCGGTGGTTGACTTGATTGGCAAATGTGCATACTGGGGAAAAGATTTATATGAATATGTATTTAAATTAACTTTGCTTAGGTGCAAAAAACTGGTAGATATAATAATACTCAAAAATGATAATCTATTCCTTTGTAAATATGAGTGAAATAAAACTTGTGTATTTCACCAAATTCCTTTTAATATTAATATAATAATAATAATTTCTTGTAAAATATTCGCTTCTCAATACGAATACTCATAATAGACTTTGTTGATATTTTAAACATCGTGTTAATCATTCGGTAGGGTGTGTACTGTAAAGAAGTGATAAAATCTATGACACACACGGAAATTCTTTTAGTTCATAAGCCAAATATTTGTCACAATTTATATTACATTTATCAGCATTGGCCCATGGCGAATTCAGTGAGCTTGTTTGCGATTTGCTCCTTTCCCAACATTTTTTTTTTGCTGAGACGACAGTGCGTATGCGTAATATGCGTAAATTGCTGTCGCCAGCAATTTGAGCCGTCAAGGAAGCATAAATTAACGCGAATTCCACTTCAGGCATCAGGCCTCCTTCATGCAGAGCCTTTGATCTGTAATTTGATTGATTGAATGAAGCTGGTGAATGGCACTTGAGCGGTGTCGATAAATCAGGGTATCCCTTCATGCTTTTTGACTATGAACGAAAAGCGGAATAGCTGTGTGTATTTTCCCGTTGCTAGAGTAAACAATGATTTTGCCTTTACAAGTTAGCAAAACGATTTTGCATTTCGCACTGCAAATCGTCGAATTGAAGATTTAGACATTTTAGCCCTGTAATGAACAGTTCCGACATTTTAGCTTTTGGCGAAACTTTTACAATGCACCATAAAATGCATTTTAATCGCTTGTTCGCTGAAATCAGGTATAAAGGTGCAATGTGCACAAACAAATAATGAAAACAAAAATGAATAAAGTCATTGCGATGCCTAACGA

TCCCCGGGAATTGTCAATCCCATTTTTGTGTTCTATTCCAAGCGTGTGTTTTATATATATATATATATATTTCCATTGGAAAAAAGCCCTACATTCATATAGTTTCATTTCACACGGAGACAAATGAGTTTCCTGTGTCAACGGCGCCCCAAAAACAATCAAATCGAAACACATTCGTGCCCCACAAATGCAATATTTTATGATACCAATAAAAGGAAACACTTAATCTAAGTCACCATTGACAGTCACATATTTCACTAAGTAGATAAAAATACAGGGATCTGTACTTTAGTTTATATTAGTATAAATATACAAATGTTAAATATATGAACGAATATAAAGTGAATATACACAATATTCAATGGAGAAATTTGAGGAGCATCCTTTATAATATAAACATTCAAAAAAATGAATTAAATTTTTAAATATCCATATAATAGATATTTCCTTTTTATAATTGAGCATTTTGCGAACCAAAATAATTATTCATTGCTATTGGTCGGCGTTCGAATCAAAGCGTTTAAGGACCGAGTCACGTCTTAGATAACCAAAATCGATAGTTGTACAACCATATGGAGCAGCTTTGAGGTATCTTGATTGAAAGTTTCGGTTTATAAAAGTGGCAATTAATTAAAAGCAATTCGTTATTACATTTTATCGATCTTATACCCAACTTATATTCGTAGCTTATTAAAGAACCCAACGAACTGTAGGCAATTTGGGCGCTAATGGCAAGGAAAAGCATTTACTTTCCGATTTATGAGCTTCGAAATGCCATAAAGCTAAGCTATCCGAAATTTACGGAGCTCTCAACTATTTCAGATGCCTGGCGACCAACAATCTGGTGGCTCTACTGGGCATGCTGACCACGACGCTGCTGAAAATGTATCTCTCAAAGGAAGTCCTGCAGTCGTTCATCCGAGTGGATTGCGTGGGACTCGTGGTTTGGCGATTTTTCGGGCTCAGTTCTGGATG

 

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