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CRIMIC CR01673

Gene CG31999

CG31999

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	4:227748..215009	102A8-102B1	FBti0212943	91271

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	long band	TCACAACACGAGGGGATCATTCCGAT	TATGCAGACAGCGTTAGATGGGCAGA

Images

Construct Design Sequences

sgRNA sequence	TGAAGATGGCAAAAACATTATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTGTTGCGCATTCATCGACA	TATGGCTACGCACACTACCG	GCCATCTTCAGTGCTGTGGT	ACCGTTAGGGCCGTAGATCT

 

Amplicon 5'

Amplicon 3'

CTGTTGCGCATTCATCGACATCACGACACGTATTCATATCTGCAGCCAATATATATCCACGTGGACAGGAACATTGATACGAACCAGGAGTGTTTATGCATAATCTTAAATATAATGTTTATAATAGTAATACCATTAATGTAATATAAAAACGTTAGTAAATACAGACCCCATACATAGTTTGTAATCCTTATGTACTTCGCATTCATTTATATCATTGCATGTCCGGTTGTCCGGTCCCAATTGGTATCCAGAGTTACAAGTGCATCGATATCCTCCCCAAAAGTTGAGACATTTTTGTTGACAAAGTCCCGGAATCACTTGACACTCATCGATATCAAAACATTGTGTGCTATTATTACCATCAGATTTCTTTTGAAAGCCAGCTTTGCATTCGCAGTAGTAAGCTCCAATAGTGTTAAAGCACTGGGCGTTCAATGGACATACACGTTGTTCTAGAGCGCATTCGTTCGTGTCAACACATGTACTAGCACCACCAATGGATCGTTTGAGCTCATGACCTATCGGGCAGGAGCACACAAAGCCACCGTTTCGATTACGGCAAATCTGTCTTTCGCCACAATTGTGTTCACCAGTATCGCACTCATCAATGTCTATTTAAAAAGAGTATACATACAATACAATAAGAATAATATTAATATTGAATACCTTTAGAATTACGGAAATACTTAATTAAAACATAAAAACGATACTAGCGGACTAAACGATTTAGCTAGCGGAAGAGCGAGGGAGAAATATCCAATATCCTTTGTCATAAAATCCAAAAAAAAAGGAAAATTAAAAAATTTAAATCATGTGTGGACTGCTTTTGTCGGTAGTGTGCGTAGCCATA

GCCATCTTCAGTGCTGTGGTATTTTTCTTTGATTTTCGAGATCACATTTATGACTGGTGGAATGTTCAAGGCTTTTTGGGTGTTTTCATTTCAAACCTACCATGTTGCTGATTCTCAAAATCTTTGAGTGTGCTCGCTACGATGTCAGTGTTGCATTCCCCCATAGCTCTGAGGTCCAGATTGGTTTTAGTAGAGTATTGTATAGTATGAGCTTGTGGTCTAGGCTAAGCGAGGAGCACGCGTTAATGAGCCAATGTAGATTGCTGTCTTTTGTCACTTAGTTTAAGTTGAGTTCAATGGCTTCAATATGCCTGCGCCATGTGAGTCTTATGTCAAGGTATACTTTTAGGTAAGTTACCTCTTCAGCTCCTGGGACTGGTATGTGTTGTTCAAAGTGGTGGACAATCTTGCCTATTTAGCGTAAATGTTACATGCTTGCAATTTTACTTGTTTTCTTTTAGATCTCAGGAACTAAAAAATCTAGAAGGTTGAGATAAAACATACCGAAACATATACGCAGCGCAGGTTTGTTGTTTCACGTTGACCACGGCCAAAGAACCGCCCAAAACTACCCCGCCCAAAAATATTTTGATATATTTTCATAATCTTTTTAGTCTTGCAAATTTCTATCGATTTCTCCACGCCCACACATTTAGATCCTTTAAACGATTACAATTTTCATGAAATAACACATTTTTGAATGTTATGTATTTTTTATAAATAACGCGACGATAATTCAAAACGGACACTTACCTATGCATGATTTACCGTCCACTGTTGATTTGTAGCCGGGCGAGCACTGGAGCAGACTTTCGCAGCGGAAATGGCCATTTGTGTTAATGCATCGTTCATGGTTTCCGCACGGATTTTGTTCCATGCATTCATTTGTATCTTTCAAAAAGACGGAAAATAAAACCGATGTTTTCGATGTTTTCTGTATCAACTAACTGTGTAGTTGTTAAGATCTACGGCCCTAACGGT

 

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