CR01674

CRIMIC CR01674

Gene CG32532

CG32532

Information

Donor Location Cytology FBti Stock number
pM37-p1X:19531851..1954748218C8Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
short band long band AACAACAACAGCAGCAGCAACAGCAG ATGCACGGTTGCCTCGTTTGAAGTAG

Images

Construct Design Sequences

sgRNA sequence AGGACACAGGACACAGGAGTCGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
TGTCATGGCCTTGCTAATAGT CCTGTGTCCTGTGTCCTCTG CGGTACTGGCTGGCGATAAG GCAGCTGATCAACAACGGAG
 
Amplicon 5' Amplicon 3'
TGTCATGGCCTTGCTAATAGTACTAGTTTTTTTTTAATATCTAACACTAGTAATGAACTTAAAAAAGTTGCCCCAAACATTTGAAAAGATGCCTGTGACTTTGATTGGTAATAAATGAATATTTGTAAGATATTTATTTTCTCTTCAAGTGAAATCTTCTAAAGATTTCATTAGTTTGGTAAATGCAATAAGTATTAGGCATTAAAAGGTTATGCTAATTATATTTTAAGCGCTTTTAAAGAGGAAGCCTCTTTCCAGCTATAATTGAAAACTTTAAAGTTGAGGCATCAATCGTCACTTATATTTGTATTCAAATGTATCAAATTTTTTCTACTAAAAAATAGTTGCCATAATCCATTGTCATAATGCAGAAAGTAAAAGTTTGTTAAATAGAAAATGGATATCGGAAATGTGATCTTCTTTCTTCTCTTAAATACTCCCTATATAAATTTAAGTGACTAATTGCGGGAAAGTTTATCCTTTATCTTCAACTCCATTACTCCCAAAATAATAGAATAGAATAATGACACCACCCACCGTGAAAATATTTAATATTGTGCAATTAGAAAATCCTTATTGCCATTTAAACCTAATTGGGAAATGAGCTTCTAGATCAACAGGACACTAAATACGTCATAATATTTGGCGGCAAAAAGTTTTGACAAAATGATTATGCAGATGCGCTTCGTCACTACGTGAACTGCCGTGCACAGTGATGTAAGCGACTTCTTTGGCTAATGATGATGGTTAATTAAGTAGCCTGTAGCAATTTCCAAACCATCATCATCGTTCAATAAACCAAACATACATTGATTCTTGGGCGGAGATAATCGCGTCCTTACTCTCAAATATATATGTACATGTGGATATCCTGACAATTCACTGGCTTTTATGTGACTTAAGTGTGATGTGTTCAATGGGAAATCACTTGGATTAGAGGCTGACCAGCTGTGCCGCAGACTAATCCATGCCACGAGTGGTGGGGCGACCGGAGCAGGACACAGAGGACACAGGACACAGG CGGTACTGGCTGGCGATAAGCCAGGAACAAAGAAGCGAGGACAAGCCGGTAATAGGAAGCTGCAAACGAGCATCAACAAACAAGCGGATGACAAAGGACACGGGTCAAAGGACAAAGGACTCAGTGGCCAAGGGGGGGGGGTGTGGAAGTGAAAGGGGTGCTGGTGCTGGTGCCAAAGGATAATTTCCTGCTAATGGGCAATGGCTAATGGCTAATGGCCATTGTGGCCAGATTAGGAGCCCAGATGCGATCGGGCCACACTCACCCGAGATCGTGTGATCGGAGAGGCGCGACGGCGGCGTGTGGGCCGGCGTATGGTGGTGCGAGGACGAGTCCATGCCGGGCGTGGGCAGGCCGGAGTAGAGGGCGCTGAAATCCTCCGGCGTGCTGCTACACTTGCGCTGGTGATTAAGCGATGAGGATGACGACTTCTCCAGCGTGGTAATTGGCGAACGCGTATCCTGCACGGAGCTGGCCAGGCTGAGCAGCCGGTTCTCGTGCGAGCCGCCGATGCCCATTAGGCGCTGGTCCTGGAACGACATGAACTTGGTGTCCTGCCCCACGCCGCCGTGCTGCATGCTCGCGTGATCCTCCAGCAGGCCCTGTTGCAGACCCAGCAGTCCGCCGACCAGGTTCGGACGCAGCTCGTCCATGCTTAGCTTGCCGCCCTGCCCGCCGGCCGAGAAGTTCAGCTGGCGGCTGAAGATCGCCGGAAAGATGTTGAACTGCTGCGAGTCGGCGCTCGACTGCGGACTCAGGCCCAGGCTGTGCGGCGCCACGTGCGGCAGCGACGAGGAGGAGGAGGAGGCTGCTGCGGCTGCCGCTGCTGCTGCTGCTGCGGCCGAAACCGATCCTGTTCCCGCTCCGGACGAGCTGGGTCCGTTGCCGATAAGCAGCGTGGGTGTGCCGGCGGAGACGCCTCCGTTGTTGATCAGCTGC
 

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