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CRIMIC CR01674

Gene CG32532

CG32532

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	X:19531851..19547482	18C8

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AACAACAACAGCAGCAGCAACAGCAG	ATGCACGGTTGCCTCGTTTGAAGTAG

Images

Construct Design Sequences

sgRNA sequence	AGGACACAGGACACAGGAGTCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGTCATGGCCTTGCTAATAGT	CCTGTGTCCTGTGTCCTCTG	CGGTACTGGCTGGCGATAAG	GCAGCTGATCAACAACGGAG

 

Amplicon 5'

Amplicon 3'

TGTCATGGCCTTGCTAATAGTACTAGTTTTTTTTTAATATCTAACACTAGTAATGAACTTAAAAAAGTTGCCCCAAACATTTGAAAAGATGCCTGTGACTTTGATTGGTAATAAATGAATATTTGTAAGATATTTATTTTCTCTTCAAGTGAAATCTTCTAAAGATTTCATTAGTTTGGTAAATGCAATAAGTATTAGGCATTAAAAGGTTATGCTAATTATATTTTAAGCGCTTTTAAAGAGGAAGCCTCTTTCCAGCTATAATTGAAAACTTTAAAGTTGAGGCATCAATCGTCACTTATATTTGTATTCAAATGTATCAAATTTTTTCTACTAAAAAATAGTTGCCATAATCCATTGTCATAATGCAGAAAGTAAAAGTTTGTTAAATAGAAAATGGATATCGGAAATGTGATCTTCTTTCTTCTCTTAAATACTCCCTATATAAATTTAAGTGACTAATTGCGGGAAAGTTTATCCTTTATCTTCAACTCCATTACTCCCAAAATAATAGAATAGAATAATGACACCACCCACCGTGAAAATATTTAATATTGTGCAATTAGAAAATCCTTATTGCCATTTAAACCTAATTGGGAAATGAGCTTCTAGATCAACAGGACACTAAATACGTCATAATATTTGGCGGCAAAAAGTTTTGACAAAATGATTATGCAGATGCGCTTCGTCACTACGTGAACTGCCGTGCACAGTGATGTAAGCGACTTCTTTGGCTAATGATGATGGTTAATTAAGTAGCCTGTAGCAATTTCCAAACCATCATCATCGTTCAATAAACCAAACATACATTGATTCTTGGGCGGAGATAATCGCGTCCTTACTCTCAAATATATATGTACATGTGGATATCCTGACAATTCACTGGCTTTTATGTGACTTAAGTGTGATGTGTTCAATGGGAAATCACTTGGATTAGAGGCTGACCAGCTGTGCCGCAGACTAATCCATGCCACGAGTGGTGGGGCGACCGGAGCAGGACACAGAGGACACAGGACACAGG

CGGTACTGGCTGGCGATAAGCCAGGAACAAAGAAGCGAGGACAAGCCGGTAATAGGAAGCTGCAAACGAGCATCAACAAACAAGCGGATGACAAAGGACACGGGTCAAAGGACAAAGGACTCAGTGGCCAAGGGGGGGGGGTGTGGAAGTGAAAGGGGTGCTGGTGCTGGTGCCAAAGGATAATTTCCTGCTAATGGGCAATGGCTAATGGCTAATGGCCATTGTGGCCAGATTAGGAGCCCAGATGCGATCGGGCCACACTCACCCGAGATCGTGTGATCGGAGAGGCGCGACGGCGGCGTGTGGGCCGGCGTATGGTGGTGCGAGGACGAGTCCATGCCGGGCGTGGGCAGGCCGGAGTAGAGGGCGCTGAAATCCTCCGGCGTGCTGCTACACTTGCGCTGGTGATTAAGCGATGAGGATGACGACTTCTCCAGCGTGGTAATTGGCGAACGCGTATCCTGCACGGAGCTGGCCAGGCTGAGCAGCCGGTTCTCGTGCGAGCCGCCGATGCCCATTAGGCGCTGGTCCTGGAACGACATGAACTTGGTGTCCTGCCCCACGCCGCCGTGCTGCATGCTCGCGTGATCCTCCAGCAGGCCCTGTTGCAGACCCAGCAGTCCGCCGACCAGGTTCGGACGCAGCTCGTCCATGCTTAGCTTGCCGCCCTGCCCGCCGGCCGAGAAGTTCAGCTGGCGGCTGAAGATCGCCGGAAAGATGTTGAACTGCTGCGAGTCGGCGCTCGACTGCGGACTCAGGCCCAGGCTGTGCGGCGCCACGTGCGGCAGCGACGAGGAGGAGGAGGAGGCTGCTGCGGCTGCCGCTGCTGCTGCTGCTGCGGCCGAAACCGATCCTGTTCCCGCTCCGGACGAGCTGGGTCCGTTGCCGATAAGCAGCGTGGGTGTGCCGGCGGAGACGCCTCCGTTGTTGATCAGCTGC

 

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