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CRIMIC CR01679

Gene Gyg

CG44244

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:21200483..21210821	57D1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAGTTGGAGATCTGCACACACCACTT	CTTCTGCTGCTGGTAGAACTCATGCT

Images

Construct Design Sequences

sgRNA sequence	GGAAGACATAAGATCATTTGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGTGACGCTGACGACAAATG	GCACCAAGTAACGCTGGAA	TCCCTTGTTTTTGAACGCCAG	CGAATGCGTGCTAGTGTGTG

 

Amplicon 5'

Amplicon 3'

GGTGACGCTGACGACAAATGACACGTACTCACTGGGTGCCTTGGTCTTGGCCCACTCACTGAAAAGGGCCAAGACCGCCCACCAGCTGGCCGTTCTGGTCACGCCCAATGTCTCGCAGGCGATGCGCGACCGACTGAAGGAGGTCTACAATGTGGTGCAGGAGGTCAATGTGCTCGACTCACAGGATGCCGCCAATCTGGCGCTCCTCTCGCGACCCGAACTAGGCGTGACCTTCACAAAACTCCACTGCTGGCGCCTCGTTCAGTTTGAGAAGTGCGTCTTCCTGGATGCGGACACACTGGTAAGATATGAAGTATTCCAAGGGCACAACATGTAACTTGTGAGCTAACAAAGACTTAAATAACTACTTTCAATTTGATTTCGTAGGTCTTGCAAAACTGCGATGAGCTCTTCGAGCGCGAGGAACTGTCGGCTGCTCCGGATGTCAGCTGGCCGGATTGCTTCAATTCCGGCGTGTTTGTGTTCAAACCTAGCGTGGACACCTTTGCCCAGATCACAGAGTTTGCCGTGAAGAATGGCAGCTTCGATGGCGGTGACCAGGGTCTGCTGAATCAGTTCTTTGCCGACTGGTCGACGGCGGACATTAAGAAGCATTTGCCATTTGTGTACAATGTGACGGCTTATGCCTCGTACTGTTATCTGCCCGCCTTCAAACAGTAAGTTCGCTATCTCTGTGGAATGGAAAAGCAGTTTGTAATGGCATTTGTTTGCTTGCAGGTTCAGAGATAAGATTAAGATTTTGCATTTTGCCGGCAAGCTGAAGCCCTGGCTGATTCAGTTCAACTCGGAAACAAAGGTGGCCAGCGTTTCGTCGGAGTATGCTCATGCCCAGGATTTGATTCAGCTGTGGTGGAACATCTTTTGCGAAAATGTCATCCAGTCCCTGTCCACCGAAATGGTAAGTGCTATTTATACAATGGATAACGCACATTTTGGAAATTTCCAGCGTTACTTGGTGC

TCCCTTGTTTTTGAACGCCAGACTGAAGCTAACCCTTTGATTTTTACTGTCTAACCCAATGTATTTGCTCCAAATCTCAAAAACAAAAAACCCTGCTTCGTTTTCTAACACGCTAATCGCAAGCAAACGCCAGGAAATGTTGCTAGTGATCGACCCGCGGGAGAAATGGCCCAAGGGTCCACTACTTTAGAGGTAAGCAGATCTTCAATTGCCGAATCGCACATATCGCACCAGATCTGTAGATACCTTGAGCACAGCCCTGTGTAAATATCTTAATTATAACTTAAATATTTATTTATTTATTTAGCTAACGATTCAGTCATCTTTCTGTGCGCCAAGTAAATAACCCAAGCAAACAAACTCAATTTCGATCTATTTGCCATTTGTTTTTTGCCGATAGCTGGCCTCGATATCGTTTCATTTCCCGCCTCTTTTCTATCAACTCGGTTTGAATATACTTTGTTTTCGTTTTATTTATTTTTATTTTTTTGTATTGGTTTGCAATTTTCTGTTGATTTTTTCTCGATTAGCAGCCACGCCCCAGAGTAACCCATTTTCCTTTTTTTTGTATATTTTCTTTCACTTTTTTGGCACCATAGGCAGACCAATTCTCAATTTATTTCTTCTGTATTTTTTCTGTAATATTTGTGCAACTGTGTGTGTCTCTCGTGTGTGTGTGTGTGTGTGAGTGCTCGTCTTAGTGTTCACTTTCTTTCTGTTTTCTTTTCCTTTCTAATTTGCATCGATTTGGCAAAGAGCCAGGAAAATGTTTCGGTTTACGCCATCGATAGATGGAAATGATTCAAATCAAGATGGTCGGTTGCGGGTGATGCCAAAAAAAAAACTCAACTGAATCATTAGATATATCCCATAACACAAACAAACACACACACACACACACTAGCACGCATTCG

 

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