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CRIMIC CR01680

Gene Ubr1

CG9086

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	X:16968679..16956232	15C4-15C6	FBti0212944	91272

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGACATTTGCCTCGTGTCGTGTGTCT	AGCTCGCAGTACTGATCCTTCTTCCA

Images

Construct Design Sequences

sgRNA sequence	GCGGCTATATTATTGGGCCTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CAGTGCGGAGGAAGGATTGT	TAGCCGCGATCATGATCATT	GCGACCGCAAAAGAGAACC	CAGTACAACAGTGGGTGCCA

 

Amplicon 5'

Amplicon 3'

CAGTGCGGAGGAAGGATTGTCGCCCAGCATAAACTCCACAACGACATCGATGATCTCCTCCTTGGCCAGCGACTCCTTAAACATGCACTTTACTGGAAGAGATTACACGCTTCATTAGTCTTAATCGAATCAAAGTGAGCTCAATGAGTTGCGCAATGCGGAGTATAGTAAGAGCCTAGCTGGGGTTCTAGATCCGCAAAACAGCCTCTTGATCTCTGGCTCAATGCGCAGGTTCTCCGTGTTCGTAGGCCCTTGGCTCCAACAATGCTCGTGGTGATTACACCCACACTCCGAACAGTTTGTCCCAAGATAGCCCACACAGTTGAAATAGTATTGGATTGGATTGGATTTGATTGATGAATGGGGAATGAGTGGGGGAGGATGAAGGGTTACGATTAGAGCGACCTTGTGTGGATAGGTGTCTGTGGTGGCTGTTCAATCAGGACTCTAGTTTAACCGTGGCTTAGGTAGGTGTTCACCTAACGTTGGCAAGTCATTTCTGGAACTTAACAATTGTGCTGTACTCACGAGTAATCACATTGGTATCTGAAAGGAAATTAATTTGTTAAAGTTAGTAACATATTAGAATCTTAATTGCATCAAGGACTATAAACAGGTTTCATAAAGTGCATGGACCTTGTCTTATTATCCTTATTTATCTAGTTGAGTAATTAAAGTCTAAATTTATGTTTGAAAACAAATATATATATTTAAAAATATTGTCCGATACTTTTAGAAAATTATAACTTTGCTGATTGGCTAAAAAAACTGTTTTTCAGTTGGCCCACAATCTTTTAGTTAAATGCGATTAAAGACGGCTCGAAGACATTAGAATTACTGGTTATAAATAAACATGCTGACTCATGGGTTAGCAAACTGTCATCTTCTGGCTTTATTGCTTTTCAATGCCGTGAGTAATGCAATAGCGATGGAGTATCGTTAAAATGATTAGAACAGGTCGGACCACACCGCATGTGGATAATGAACGTGATAATGATCATGATCGCGGCTA

GCGACCGCAAAAGAGAACCACAGTCGCAGACAAGAGAAACCTTCGGGCTAAACGAGTTTTCGCAACCAGAGAAACCAAATAAAATCAAAATCAAATTCAAATTCAAATGCAAATACAAATACAAAAGCCAGCAATTTACAACAAATGCAGTTCAAGGTCGCCACAAACGATGTGAAAACTCTGCAGCGCAGAATCGAAAAAAAAAATACAATACTAAAAAGAGAGCGATAAGCTTACAATATAATCTATTATCAACGCATCTAACGTAGGGCTATCAGTGAAAAATGAAATATTTGTATGCATATGTACATACATATCTACTGCCTGCAATGCACATACAATAAAGACAAATTGAAAGTAACTAGCAGAGTACCTCCTGTACACTTTAAAGTTCCTTTAAATAATCTTCACGCTGCATTTTGCTATATCTCCAGGTAACATGGCTGCCTATATAAAAATGCATTTAAAACTGCGTGTGCGTCAGTTTACTGAGGCGTAATAGACTGGAACAAAGAAGGCGCCTGGAGCGGAAAATAAAAGAAAGAAATCGAAATCGAATCGAACTGAACTGAACTGAAATGCACTATACAGAAATGCCTCTATACTATGCCAGGAGGAAGAACGCCAGAGAAGAGGCCACCTCATCGGTTCCTGAATCACAGTGGATTATTCAGGAGCGGTACACTCAATTCTACAGGCACGATCACAAAAAGAGATCAAATGTGTGTAACAATATGATATAAAGATCACTTTTTAAACAATAATTACAAAAGTAAATACAATGTTTCCTAAAGTTTTTAGCAAAAGTACTCAATTAAACTCTCAACAAGACGTCATCAAAAGGTGACTGAATCGAAAATATATGCATAATTTAAACGATATTAAACTTGACTGCAAACATCATACGTAATTACAGCTTCCAATGAATTGTGCATATTTTTCGAACCAATAAATCTTGGTCATTCGCCCTTAGCATGGAAATTAATTATGGCACCCACTGTTGTACTG

 

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