• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR01685

Gene Lgr1

CG7665

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3R:17729747..17729751	90C2	FBti0210478	86491

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCTGAAGTGTCTGTCATTCGAGTTCC	AATCGAAGGAAGCGAAAGTGATGGGG

Images

Construct Design Sequences

sgRNA sequence	ACTTGGCCGCCACCCTACGAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACTGTCACGACATCCACCAC	GAAGGCCCCATGATGAATGC	GGGTGGCGGCCAAGTTAATA	GGATCGTGCCGAGATGGAAA

 

Amplicon 5'

Amplicon 3'

ACTGTCACGACATCCACCACGGATTCGATGTCTACCCCAATCTCACGGCCGTATCGCTGGCTCAATCGACGGACACGCCCCTGACCGCGACGATGCCACGGTCCGCCTGGAAGTGTTGTTGCTGGAATGCAAGTAATCAAGTAAGTGACTGATAGACGCACACTTTCTACTTAATTCTATATGTTTACCAGCTATGCCAGCGGCCTCCAAAGTCAACTGCAGAACTTGAAGGTTGGGTAAACAGTGAAACTAGAAGGCAGATCCGTTGGCTCCTGTTTTAGTTGAGTGTCCACAGCAACTTTAAGCCTTTAAGTTCCGAATGTCAGTTAATTGAAGTAGTCAAACAGCCAACATGGCATTTTCCACCGACGTTCATTGAGTTTTAGTTGTGATTTTGACTAAAATGAAGTTACCCACTAACTTTATTAATAACTACTAACAGCCAAAGTTCAAGATCACAGAATGTCAAAGGGTCGCCATCGATAGCAGGTCGTTGTAAGTGAAAGGCCTGCGCATTTATTTTTAGAATTATACTAGGAAACTAGGTTGAAATATGAGGTTAACACTGCTGGCTATATTATTTATTACTTTCCCAACTGCACGACTCCCACGCACCTGGATAAAAACATATTCCACTATTTCCGCAGGCGGAGGAGGTGGAGTGCCGCTGCGAGGGCGATGGACTGAATCGCGTACCGCAAACCCTGACGCTGCCCATACAGCGTCTAACTATCGCATCGGCGGGGCTGCCACGCCTACGCCACACGGGGCTGAAGGTCTACGGATCCACGTTGCTGGATGTGTGAGTTATATTGCGATGGATAGACATATCCACCGACATATCCATATCCTCATTCCATGCGTTTACTAATCGTCCTTTGGCTTGGCAGGGCCTTCACCGACTGCCTGCAGCTGGAACTGATACAGGATGGCGCTTTTGCTAATTTAACCCTATTGCGAACAATGTAAGGCTTGATTTTAAGTGGCGGCTTCAAGCGACCGACCGACTGACTGACTCACTTATTACCCTTTATTATCCTTTCAGCTACATCACCAACGCACCCAAGCTGACTTTCCTCTCGAAGGACGTATTCCTTGGCATTTCGGACACCGTGGACATTATGTGAGTCCAAATCTAGCTCTAGATGCCCCATCTGTGGGGCATTCATCATGGGGCCTTC

GGGTGGCGGCCAAGTTAATAAGCACACACAGCTGTGTGGTTTTTTTTTTCGGCCAGAAGATGGGGAATATGTTGTGTCATCGGTAAAAATAGCACTCAATGCCCATCTACGCGGATTTGTGCGCATTCCTACTTCAATATGTTGCCTAAATTGTCTGCTATGTCGAGCATTCCTCAAAAGCATTCTGGCTAAAAAAAAAAATGCAATTTAATGAACATGGTAGAAAAATTTATTTGTCCATCATGCAGAAATAAATATTCGATTTAATTGCTCTGCGCTAACGGCAAAATACAAATGTAGAACTATTTACTTTAAACTTTATTACACAATATTTTAATTAATCCTGCTGCGGGAAGGATGTGGGTGTGCTCATGAAGTTAACACAGTCTAACAATAGTGTCACTTTACTCCCACAGACGAATCATAAATAGTGGCCTGACCAGAGTTCCTGACTTGGGCCACCTGCCACCTCATAATATTCTGCAAATGATGTGAGTAGCAAAACCCAGCTTACCCTTTGTGGTACTAATATGTACCCTATTTTTTCCGCAGAGATCTCGATAACAATCAAATCACTCGGATAGACAGTAAATCTATAAAAGTAAAGACTGCCCAATTGTACGTATAAATGGTTTATTGCCACACATTCAAGTAATCTCTGAAGAGCTCTAATCTTTGCAGAATTTTGACAAACAATGAAATAAGCTACGTTGATGACTCCGCTTTCTTCGGCTCCAAGATAGCCAAACTGCAAGTTTAACCCAATTTCAAATAGTAAATAGTTATTAATCATGTGGCTTTTTAGATCGCTGAAGGAAAACAAAAAACTGCAAATGATGCATCCCAACGCATTCGATGGCATCATAGACATAACTGAACTGTAAGCTTGGAAATTGTATCTTTATTGCATTCGCAAGATTTAACCCTTTGTATTTGTATCTCCATAGCGATCTCTCCAGCACCTCGTTAGTTGGCCTTCCTTCTGCCGGACTTCAGAACATCGAAGCGTTGTACATTCAGAACACCCACACTTTAAAGACCATACCATCCATCTACAACTTCCGGGTGAGTAAAAGTCACTTTCTACCAAATAATTTAAGCCCAAAGTTGCGTTTTTTTTATAGAATCTGCAGAGGGCCTATCTCACCCACTCCTTCCACTGCTGCGCCTTCCAGTTTCCATCTCGGCACGATCC

 

---

Search Again