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CRIMIC CR01689

Gene Tsp66E

CG4999

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:8932065..8912499	66E3-66E4	FBti0212945	91273

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	TTTCGTGTGTGTGTGTGTGTGCTGTG	TTTGAAACGGACTGTCTGTGTGTCCG

Images

Construct Design Sequences

sgRNA sequence	CGTGAGGTTGATCTATATTTCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGAAACCTCGCAACCCAGAG	GGGTTGATTCACTACGATTTCG	AGATCAACCTCACGTGCAGG	GGGTCCACGCTACGTATACG

 

Amplicon 5'

Amplicon 3'

AGAAACCTCGCAACCCAGAGTGCTAAATGTGGAATTTCTCTTTGTCATTGCAGTTGAAGGTAGCTCCGTGGAGGAAAACAGCCCCAAGATTTCGAAAACAACGAAAATGGTCTTCGACTGCGGAGTCTGGTGTGCAAAGTATTTGCTTTGCATATTCAATTTTATATTTTTCGTAAGTACAGATGAAGTAATGTGGGAAGGGGGTTTATTATGCTATATGCACCAGGTAATGCATATGGGTAACGCATAGAGGTGCGTTCATGTCATCCGATGGTATCCCATGCCACATCGCCCATCCATCCAAATTCCATTGCAACTCAATCAAGCAAATCTCTGCGCATGCGCTGGTGCTTTTTGCATTTTCCCGCATTTTCAACTTGGCGGTGGCGGTTCATTGACTTTCAGCCAACATCGTCATCATCATCATAATCATCATTACCATCATGTTGATTGCCAAACGTCATCATCGGCATTTTCAAATGCAATCAAGATGGGCAGGCAACGTCATAATAATAACAACAATCATCAAACTGCGTTTGCATGTCCAGCGGGGGGAACTATGTTGTGTTTAATGCTTTATGTTTCAGTGTACACACATAAATGACCACTACCACTACACACATATACATATATATTGTTTAATGTTTTTTTTTTATATATATATTTATATTGCAGGTGCTAGGCACAATTATATTCGGAGTCGGACTATGGCTGGCCGTGGACAAGCACTCGCTGATTGCTCTCCTCAAGTTGGTCGAGAGCGAGCGCATTGAGGTAAGACCAAGTGAAGACGGGGGTTTCCCCAAAATCCCGCCCAACTCCGGGTGAAAGTGTTGCCGCAGAAACACCCCCTTTTTTTATGTCGAAAAGGCCCCAAGAAAGCACTGCATTCCACCCAGTTACAACGCAATTTATACGCAACCCATCGAAGTGTGTAATTCACATATCGAAATCGTAGTGAATCAACCC

AGATCAACCTCACGTGCAGGTGCTTCAATAATAGCTGGCACTGGTACTATGATTATTAGAAAAATATAATTATATTGTAAACATGCGGTCCGGGTGGAAATGGTCAAAATGTGAGGCACGAAAACGCTAATCAAAGTGCACAATGCACTGTGCTGACACACTAACAAACAATAGGGGATGGCTATAAATGCCACTTTAATTGGGTCGAAATTGTATATAAGACAGCAGAAATACAATGGAACTGAAAGTAGTGGAATCTAAAATTTAAATAGAGCGACAGGAAGAACTTTTCCACTACTTTTACATTTCGGTTTTTTAACTAATATCAATCTATAATTATACACATCAAGCATATTCAAAGTAAATTTTTCTTAACCTTATTTGTAATGGATGACAAAATGTATTTTTCATGGGTTAAGTGCATATAGACACATTCTGTTGTTGTGTCTACACATCTACATTTAATATAAACTACACAACATTCGACTAAACTTTTAGAAATGTACAAATCGTTGTTTAAAGCCACCCTCTGGGGTCCTTTACTCAATTCACACAGATAAACTTTTTCAACTATGACAATTTCCATCAAAGTTTGCATGTTCAGTGTTAACTAAAGTTTGTAGTGAAAATTCCCTTTGCTATCTGACTGGGCGTAAAGGGTTTTTCGCTTTGGCAAAACAAATGAAAAGTGCATGTCCATACGGTTACTAATTGTGGGGTCACCTAAGATAAACATGCTAAGGCTAATTGAGAACCTTTACACGGAAATGTTTCACGGGTTGCGTTTGGTCAATGAACTTGCACTCTAGCTTTAAAAGACATACATATCTCAGATCTGACAATTTGCCTACGGCAATTGGGTAAAATGTGCACATAATTCAATTATACGAATGAGGATGGAGACAATTTTTGGCCAGTTCAAAGTTTAATTACGTATACGTAGCGTGGACCC

 

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