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CRIMIC CR01692

Gene rut

CG9533

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:14826073..14787478	12F4-12F5	FBti0212947	91275

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATCTCATGAGCGCTTCCTCCTCGTTT	TGAACGAACGAACGAACGAAGGAACG

Images

Construct Design Sequences

sgRNA sequence	GTTATCGGGCTTTCAACTGGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGGATTTGTGCCTACGGTCA	AGCCCGATAACCAGTAACCA	ACTTAGACATGGGTGAGGCA	TCGACGTGTGGTCAAACGAT

 

Amplicon 5'

Amplicon 3'

GGGATTTGTGCCTACGGTCACTTGAACACCGCCCCCACCGGCAATACCACCGGAAACACAGCCACCACCACCGGAAATACCGCTGTCCACACCGCCGCCGCTGCCGACGACGCTGCCATAGCCCATGCCGTGCGGTGGCGGTGTTGCATGCAAGATCTTTTGCAGACGTATCACCTGGATCGGAGAAGTAGTATACACAGTTGTCATGGTAAGTTCTTATATTTACATTTGAACAAAGTTATTGTTTTGTGTGTGTGTGTGTGTTTCTTATCACTAGGCGTAAATTAAACAGTAGCGAGAGATAGAATTAATCATAGTAGAACGGTAAGTTTAGTTAGGGTACGAATCAAAACGATAACGATAGAAAGTGGAGAGAAGAGGCGCATCAAGTGGAAATCAAAATTGTAATAATCGAAATAATCGAAACGAAACTAAAGAATAGCTGGCTGCATTTAAGTAGCAATAATATATGTACATAACGAGTGAGTATGGAGTAGTATTTTAGTATAAAATACAGTCTTATCACTAGCGGAACTGAACCACCTATAAAATATATCGTCGTCAAGGACGAAACTACTTTCCAAATTATCGCCAGCGAATTGATCGATTTAACCGATTACTTATGTTAGTGCTAATATTCTGTAAAGTTCAATGCAATTTTAATTAATATATAAATTACTAATATGTAAAATGTAAGTGTGTCCATTTTGTTTCCGATGACAACAATTTAGATCAATTTACAAGTCCGAGGCGCAGATATATACATAAATATATATATATTTTTTTTCTACAATCAAACTACACACAACATCGACGCAAACGAGCCGTTATACTACAATTGTTAGTTGAGACAGCATTACGAATTACAATTGCTGATCTTGCTAAGCTAAAAATACAATCTTATTTAATATATAGGAATAGATACATTTTTGTACAATGCGACTACGAAACGAAAAGAGCCCATCAAATACGGAAAACGATAACCGATCGAGAAATTAAAAAAAAAAAAAAAAAAGGGGGCCAGGGGAATAAGCGCAACTGAGGCATAATGGTTACTGGTTATCGGGCT

ACTTAGACATGGGTGAGGCAAACTTACCGCATCCTGAAAGAAAACGAAAGGAAACAAATCAAAGCAAGAAATGAAAAGGATTCTCGCCGTCTTGATGCTACCCGATATCTATCTAACGAATCATTGTGCATCCCTGGGCTACGGATACAAGATACTAGTACGGAAAATTAAAAGCACACCAAGCCACACCACCCGTTAACGGGAATTCGGTATCCGGGATCCGGAAACTTAATCCCTCAAAAACCAACCGGACATGCAGAGAATTCTGGAGCTACGACAAAAGAAATAGAACAGAACCGAGCTACAGGAACACGCAACTTTCAACTTTCAACTTTCAACTTCCAACTTCCAACTTTCCAACTCCTCCCACCTTGAAACTCCCGACTCGGCTTCTCAGATTGGGATGCTGAACTTTAGATAAACAACACCACTTACCTTGCGCGATCCCTTCTCCGACACCTGAACGTTTCCAGAGTTCGCCTCGCAGGTGCTACCCCGTGCCACGCCTCCTCCTCCTCCTCCTCCACCACCGCCGACTGAAACTCCGCCACCCAAGGCGCTGGCCGCTGAGGGGAACGATCCGGTGGCTATATTGGAGAACGGCACCGGTTCCGAGAACTTGATCGTGTGCAGCAGCTGCATGACCACATTGGACACGTTTCTGAACGACAACTTCCGTCGACTGCCGATCGCCGAGCGAACGCCCAGTGTGTTGAGCATCAGTGGCTGCAGAAGATACGAACAGATTAGGCGAATTCACGAAAAGATCAGAACAAATAGGTAACAAAAGCAGAACCCTTAAGAAATCAAGCACATAAGTATAAACAAAGATGTTGCTCACCTTGCGTCGATGTGGCGGCGGAACGATGAAGAACGTGTCCACACCATGGTCGCGCAGATACGAGGACCGTTCGTCGCCGTGGCCAGCCTCCACCTCGTATTCGCCGGACAAGGAGTCCAGGGTGGCGCGGGTTACGTGCACCCGACCCGGTTCCCCGCCGGACTCCATGTGATTGGCCAGGGTCACATCGTTTGACCACACGTCGA

 

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