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CRIMIC CR01694

Gene sbm

CG9413

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	X:14602580..14584733	12F1	FBti0212948	91276

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGCAGGACCAGAATCGTGTACTCGAA	CGCCTGAAAGTGGGCAATCAAGGATA

Images

Construct Design Sequences

sgRNA sequence	AAAGGGCAGCCCTAGAAAATTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGGAAGGTGTGAGATTCCGC	AATTGGACCCCATACAGCGG	GCCCTTTTCGCCCCCTAAAA	TGGCTAAAAACGCGCTATTCA

 

Amplicon 5'

Amplicon 3'

TGGAAGGTGTGAGATTCCGCGAAAGTTTGGCTCCTCGCCAGCCGTGCAACAAGTTGTGACGAGAGTGCAGGGTCGAATTTTGGGACTTGGCCACTTGACGTCGTTAAGTGGGTCAGTTGCACGTGGCGCACATTTCGGGCAGGAGGGGATTTTATGGTTCTGGTGACTACTTACCCAGCAGAGAGAGCACACCACAGGCAAGCCAGATTATAAAGCTAACTCCAACGGAACCAGTGCGAACCAGTAAACCGGAGGGTGACACAAAAATTCCAGACCCTGTGGATTGACAAAAGGGAGAAGATATAGATACAGAATATAGATATAGATATATGTAGCTGCGAATATAGATATATGTTGATATAAGTAAAATGCTGCCACAGCTGCGCTTCGGAAAAAACAGCTGATTAAGGGCCCACATTGTTTTCCCTACTTTCCCCATCGTTTCGATTTCCCGTATGCTAATGAGCGGCTGGCGGGGCTCTACATAGTACATATATACCATTTGAGTGGGCGTTGCGGACGTCATCAGTGGGCGGACAAAAGTTTCGGCGAAAGCTCCGACCACGCGGCGAATGCGCAATACCGGCAAATAGTGGTTTTTCCTTGCAGAGAAACTCACACAGAGGTTGTATGAGGCCGGCGAAGCGGTATGGAAAAAATATATGAAGTTCGTTGGATATTCGATCCAGAGGCGTGCAACAATTTCGGCTGCGTGCCACAAGCTGGCGCCATTGCCTAAGGTCAAAGTGCAGCAGCGACAACAGACGCCATTCGTGTCATAAATATGTATTTCAATACAGCCCAGTTTTGAAGCCAAATACAGCAGCGTCCCAATTTGTAGTGATAACTGGGCAGAAAGTATAATTGCCAATACTACGCTTTTATATAAAGTGATCTATTTTGAATTACATATATTTGTGCTATTAATTAATTAATTTCTTGTTTTATAAAGATTCAAACAAGAGATGTTCTTTTTACCAAACACTAAAATATACAAATATACTTTGATATATTACTGTGATTGACCACCGCTGTATGGGGTCCAATT

GCCCTTTTCGCCCCCTAAAAATATGCCTGGAAATTGGCCTTAATCCACGCCATCCTCCGCACTCCTCCCACCAGCCGTCTGATGGCACTTTCATTCTCAGGCCGCTTGCGTACCCATCTACATATATATCCATACAGACAGACAATACATACATACACATATATATATATAGGTGGGCCTAAAGCTGTGTATATGTATATGTACTATGTGCATATACAGTATAATTCGCTTAGCTGCATCGATAGTTAGCTGCATCGGCAAGATATCTGCATTATTTTTCCATTTTTTTGTGTGAATAGAAAATAGAAAATAGAAAAAAAAAAATTAAGTTAGCTGCATTTTTAAGTTACCTGCATCGAGGCATTGTGCAAAGTACTCGAGGCAGCTAAGCGAATTATACTGTAGCTAAATTTATGCGTACGTATTTTTGGCCATGCGCGTCTCCAATTTGAACCAAAAAAAAAAGGCAAGGAAAATAAACCGAATGAAAATTGTGCCTCAATATTTTTCACCGACTCCCAGAGCGAAAGATGTATTAAAAAAAAAAAACAAGAACGAACGGAACAGCAAATTTGACACAATCCGTGGAAAACTGCGTGTTCGTGTGTTTTTTTGGGCGACACTTTGGATTTTCTCTTTTCCCGTCGCTTCGACATTCCTGCCTCATGTTTTATGCATTTGTTTTGACAATTGCTTTGGAGCGAAGCATCCCCGAATGGATTGTACACTCACCTATCATAGTTCCAACAATTAAAGCCACCCCACTGAAGAGGCCCAGCCTGAAATGACAAAAAAAAAGAGAATAATATTGTAAATTCAACGAAATTATCAAATAAATTAAAAATACTGCTTTTTGGGGTAGATTTTTAAGGTAAAGACATGATTTCATCACTCGAATCTGCATGTTAAAGTCAAAAGTCGATAAATATTGACGGATGGATGTCAAAAATAGATCCGTGTACTTGTTAATTTTGAGTATGTTAAGTAAATCACTTAATTGACAGCAAGGTGTAAATCAAGTGAATAGCGCGTTTTTAGCCA

 

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