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CRIMIC CR01695

Gene Stat92E

CG4257

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3R:20539064..20539073	92F1	FBti0210479	86492

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTTTTCTCCGCCAGCTTCCGAAACAT	GATGGTGCTATTCTGCAAGCTGCGTT

Images

Construct Design Sequences

sgRNA sequence	GCGGATCAGCCATGACATAGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACTGACGCGATGCAATGGTA	ATGGCTGATCCGCCCTTTTT	GGCTTTAACAATAATCTCGGCCC	TGAGGAGCGGTCTGAGTACA

 

Amplicon 5'

Amplicon 3'

ACTGACGCGATGCAATGGTATAAGAATGAAGTAGCCAATTGCAATTTGAACGAGGAACTGGGAGATGATGGTCAGGACGGATCGATCGGATGAGAGCGCTATTAGGGCGTGTGTGAATGCGGACGAAGATCGCGGGAAGTATCAACAAGTGAACGGGTTGCAATGATCGGAATTGAGTGATTTGAATGCGAAACTTAGCTAAAATGTTATACCTTTCGGTAAATGGCACAATCAACTAGCGCCCAGTACCCGGCCATCAGGTGCAGGCGGTGGCACAGCATAAGATCATTAATAGTAAACGTACTGTCTATAGTTGCTCGTCAATAAGGCATCAGTGATTTCACTTATCACAGGATCGTTGGGTTGAGCGCCTATCACAATTCTCTCTAGGGCCTCGGCCGTTTCGCTAGCATCGGAATCTGATTCCTCAAGTCGGGCTGGGCTATAGAAAGTGATGCCGCTTTGTGGCGAGATACTGAACCATCACCAAAAAATCAATAGCCGCAAAAGGTGGAAAATGAAAATAAACAAAGAGTTGGGTAGGTAAGTTTCTGTGTAAGTACAAATAAGTAGAGACGAGTGAAATGGAGAGCTTATCAAAGAGCTCAGAAAGAGTATCCAATTAGTTTTATTAGTTTGTTAGCAAAAGAGCCTCAGGTTCGGATTAGTCAGGAACAAACGCTTTCGGTTTCGACTTTTTGCCCCTTTACAACGTTTTTTTTACGAATTCAAAGTATTGCAATCTGATTAAACTTTTTGGTCAAAAACATAATCATAATCAATGTAAAGCAAACAAGCAAAAACAAAAAGGGCGGATCAGCCAT

GGCTTTAACAATAATCTCGGCCCAAAAATTATCTCTCCACTCAAGCTTTGAAGCCAAGTATAAACGCAATAGGTAATAAATGGTTTATTGTTTTTTGTTGTGGCTGTTGGCGATCGATTTGGCTGTGGCGTTGGCTTCCAAAACTTACTTTCCCAGTTGCATGCTTTCCTGAGCATGATGCGGTGTTCCACTAGTAGATCCATTCTCTCCGTTTCTACAAACGTGAACATGCAATGTGCTCTTCACATAACCGGTCACAGGATCTAGAACGAGGGGTTCGGGCTCTGATTAGGGTGTTGGACCGTGTACCTTTGATTGCATTCTTACCTTGACGCTTTGAGTAGAACTCACCGAAGGCGACGTCCCTCTTCACGGGACTCGCATTGCGGTCGCTAGGGTGCAGCCAGCAAAGCACGTCCAGATCTCGAATGCGATCGGCCAGGTTCAGCACCTGGAAATCCCGTGCAGTCCATGGCGCTAGCATGGTGACCAGTCCATTTTCGTTTACGTAGGCGATAGTGACTCCACCTGTAGTTTATAACATTTATTTTTTGTTATATCAAATAGGTAAGTTATTTTAACTGACCGAGCTCGCTGTCGGAGAAACGGAGCAGGAAAGTGCCGATACCATAGACTGAACGCAGCAGATCAGTCTGAGCCTTGGTCTTGTTGATGAAGCCCATAATGCAGCCTGCTTTCCACATGCCCAACATGTGATCTTTGGTGAGTTTCATGATGGCAAAGAACCACTCCCAGAATGTAAAGGACCGATCGGGCATGGGCTCCTTACAGAATTGGTTCCACGTAATGTACTCAGACCGCTCCTCA

 

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