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CRIMIC CR01700

Gene Mcad

CG12262

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:7721472..7719350	66A10	FBti0212949	91277

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	ATCTGCCTGTCAACTGTGTCACGGAT	TTAAGAGCCTCATCCAAGCAACGCTG

Images

Construct Design Sequences

sgRNA sequence	GCTAATAATACTTTCTCAGGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACCTCTGCCGACGAACATTT	GGGGGCAAAGTACAGATGAT	GGCGTCTAATGGCGGCTTAT	CTGGCCGTTGATTACCCACT

 

Amplicon 5'

Amplicon 3'

ACCTCTGCCGACGAACATTTTCAAGCCTAATATACCCGATTGTATTATTTTATACCTATTGCCCAGAAGCAATACGTTACGTTGTGAACTGAAAGCTTTTACCTTACGTAGATTGCTGGGCATTACGTTAAAGCTATGTTTTATTAAGCTTATTTCGTAGGGTATTTCGTGTGGTACATTGATATCTTAAAAAAGGGGGTGTGTCTCTTCATACACATATGCCTATGTACTTGACAAAACCTGGATGAAATGTACAGTTGGTATTTTAAAAATATTTTACAATACAATTTATTTATATAAGCATTATATTACAATTAAGGCGAGGTACAAACAAATTTGTTAACTCTTCGAAAAGCTTTGGGAAAGTTCCATCAACAGCGCATACAAATGTCTGCGAAATCTTCCCTTATCTCAACCTCCTGCATATATTTGTAGGTGCATATGCTTGTAGTTCGTTCGTCACCACGATTCTGTAGCTTTTGCGACTGCCAGCGCAACAAGGTCCAATGTCCAAGCGTCGCTGCCATATCGCGATGAACCAATGTACATATGTATGTACATATATAACATCGCTATACCGGCGCATCGGCAACTCGTTTGGATCATTTCGCTGGCAGCCACCGACGAGTTAGCAAGGCGACCTAGTTAAAACTCAGGAAAATACGAAAATAAACCCACATTCAAATGGCGTTCCTCAACAAGGTGAGCATATCAGTCAATTTACAACGCAGAGAAAGTGAAAAACACATCGCTGGGACATATCATATCCAAAGGTCCAAAGTTCTATCACGCTAGTGACGACAGCAACTGTTGATCGTAGGATCATACATACATATACATATGTACCTACCAACAAACATATATTCGAGTGGGTTCCGTTTCGAAAACAATTTGTTGCTGCCTATCTGTTAGCAATCATCTGTACTTTGCCCCC

GGCGTCTAATGGCGGCTTATCGTGCACTCTATTTAAATCGGAATGCAAACTTTATGGGCAGGCATATTCGTTATCTGTTTTATGAAAAAAATAGAATGTGATGGTTGGTTATGAACCTCAAACATCCAAAGTCCCTTGTAAATATAAAAAAAACCTTCGTCTATGATCATGATGTTGTATTAATTAAATACATACGTAGTTGGCCCATTTTATTTGTTGCTTGTAGTTAAGATTAATTCGCGGTCAACTTTGTTGTTGTTCTGATTATTTCTACATTTTCATTTTGCATTTCGATAAACACTTGTTTACCAGTTGACTTTAAATCCGATATCCAATATCCGAAAATCCAGCTTGCTGCGCCTGCCCTGCGCCAGTTGGTGTCCCAAAGTCGCGCCTACGCCGCCGTGTCACACGTTTCGCCCAATGGCACCTCTTTCGCACTCACCGAGGATCAGCTGCAGCTGCAGGAACTGGCACGTAAGTTCACCCGTGAGGAGATCATCCCGGTGGCCGCCCAGTACGACAAGAGCGGCGAGTACCCGTGGCCCATCATCAAGAAGGCCTGGGAACTGGGCCTGATGAACAACCACATTCCTGCTGATATTGGTGGCCTGGATCTCGATGTGTTCACCACCTGCTTGTCGGCCGAGGAGTTGGCCTATGGCTGCACTGGCATCATGACCGCCTTAGAGGCTAGTGGTCTGGGCGTAAGTTTAAAGTCATGTCTATTTACGGCAAGATGATCGAAACGCCACGTTCTTTCATAGCAAACTCCTGTGATCCTGTCCGGTAACAAGGAACAGAAGAAAAAGTACCTGGGCCGCCTGCTGGAGGAGCCACTTGTCGCCGCCTATTGTGTTACAGAGCCCGGAGCAGGATCCGATGTGTCCGGCATCAAGACACGCGCCGAGAAGAAGGGTGATGAGTGGGTAATCAACGGCCAG

 

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