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CRIMIC CR01701

Gene CG4678

CG4678

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:16711145..16696131	15A1-15A3	FBti0212950	91278

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTTCCAGATGCGTGTTATCGACAGAG	CTCGACCAGCCGATTGTGAATCGAAA

Images

Construct Design Sequences

sgRNA sequence	AAAGGGTTAGCCATCACCTTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCTCTTTCCGTTCCGTTCCG	AGCATAGTGCCAGTGGTGAC	GTGATGGCTAACCCTTTTGTG	CGAAATCGCCGTTCCTTAGC

 

Amplicon 5'

Amplicon 3'

TCTCTTTCCGTTCCGTTCCGCCAAACATGTCCTTTGGGCGCAGTGTTCCAGACCTACTCGGCGGCGCCATCGCTGACGCCCGACGATGATGTGTTCAAGCACCTGTCCCTGGTCTATGCCCGCAACCATGCCAAGATGTCCAGGGGCGTCGCCTGCAAGTCAGCGACGCCGGCCTTCGAGAACGGCATCACCAACGGAGCCGCCTGGTATCCACTGACCGGCGGAATGCAGGACTACAACTATGTGTGGTACGGCTGCATGGAGATTACGCTGGAGATATCCTGCTGCAAGTTTCCGCCAGCCTACGAGCTCAAGAAGTACTGGGAGGACAATCAGCTGGTGAGTAAATGATTAGAATCTATTCTTTTGAAATTATTCCATGATCTCTTCATTTCCTCCATGAAGTCACTTATCAAATTCCTGGCCGAGGCGCATCGCGGTGTCCAGGGATTCGTGTTCGATCCGGCGGGCATGCCCATTGAGCGAGCCTCCATCAAGATCAAGGGACGCGATGTGGGTTTCCAGACCACCAAGTACGGCGAGTTCTGGCGCATACTTCTGCCGGGCTACTACAAAGTGGAGGTGAGTGTCCGGCCAGGATCACTAACCATATCATATCATATGTCTAACTTAATTATGCCAATTATATTAAAGGTCTTTGCCGAGGGCTTCGCTCCTCGCGAGGTGGAGTTCGTGATCGTGGAGCAGCATCCCACGCTGCTCAATGTAACGCTGCAGCCCTCAAAGGTAGGTAACCCAACCAATCCGCGGTGGAGTGGGGTCACCACTGGCACTATGCT

GTGATGGCTAACCCTTTTGTGTGTTTTGAATCCACTTAATATCTGCCAACGACAATCAGTATCTGGTGGCTACGGGCACATCCACCACCACAAAATCCTCAACGGTGAATCGCCGCGTCAATGGAAAGCTGCAGCTGTCACCAGAATAATGGCGATCCGATTTCTTTGAGTGTGTAAATAATAGCTAAATTGGTTTAGACCTAGAAAATGATACGCAACTAACCCACTAAAACACCATTGAAGTAATCTGTTTTGGTTTTTGCTTTAGTTCCCATTGCGATTAAGTTTATGAAACAAAATAACGTTAACTTTCCAAATTATTATAGTTTAAACACTTTTAGTTTGAAAAATCTATATAAAAAAAATTAAATTGAGTAAAATTAAAATATTGATTACATTTTTGTTATTACTTTTTGTTTATCTAAAGCTTATTCTTTAAAAAAAAAACTGATTTTCTTTGTGCTTTAACAAATCTTACCAAAAAGTTGACAAATAATACCCTTAAATACAATGACCTTTGCTGGCAAAAGGTTTATTATTAAAAATATAATGGTTGCTTGTAACAATAATGTAGAAAGGGAATCAAATCCTAATCTCTGTGCACCTACGTATAACATCTTGTTGACTTCGCAGCGCTTGGAGGGCATTGGGCCCATGGGTCCCGGCGGAGTGCCAGTGGGTGGTGTAGTGGGTCCCGGTGGGCTATACCGCCCCATTCCGTCGCCGCAGCATTACCGCCCACCTGTACCGCCCTACGCGGGCGCCGCCTCCAGCGATAGTGGCATATTCTCGACCATCAGCAATGGTTTAAACAGCCTGTACTCAAATATATTCGGCTAAGGAACGGCGATTTCG

 

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