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CRIMIC CR01703

Gene Hayan

CG6361

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	X:18481303..18481313	17B5	FBti0210480	86493

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTGGTGCTGACAACTTCTGCGTATGT	TTTGATGGGAAACACCATCCGATCCG

Images

Construct Design Sequences

sgRNA sequence	AGAGACTGCATGCGGCTCCCTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GAGGAGACGCGTGTGTAGAG	CGCATGCAGTCTCTGTCTCA	CTGAGGCCAACTGATGTCCA	ATTCCATGCTACGAGCTCCG

 

Amplicon 5'

Amplicon 3'

GAGGAGACGCGTGTGTAGAGGCCCGGGGTCTTGGTGGCGCAGCCGAAGCCCGAGGAGATTACGCCTACGATCGAGTAGGTGCCGTCGACGTCGTCAATCTCCAGGATGAGAGGTCCACCGGAATCGCCCTGACAGGCGTCCTTTCGCTGATTCTTGTCCGCCGCGCACAGCTGCGAGGCAATCACGCCCCTTCTCAACGTCCGGTTGGCACTCGGCTGTTCCGCAAAGGATGCATTGCACTCGTCGGCCGGCACCAGATCCAGAGCCGCCCGCAGCAGGATCTTTGATACGGCACGATCTGTAAAGACACCATCGAACACAATCCAATCCTGCAGTTTACGCACACACAACTTACTGGTCACATTCATTACACCCCAACCGGCGACGAAATATTTGTAGTTCGCTGGCGGATCACTGCGATCCGTGTAGAGACAGGCGGGACGGATGACATCGGACTCCTTGGCATCCTCGGCCAGCTGCAGAATGGCGATATCGTAGTACTTACTACTGCCGGAATAGTCCGGATGAATTTGAACATCAATCTGCCAATGGAAGTGAGTGAGTTCAACTGGTTATCTCGCACCATGTGTCACATGGCGATACCAACCACATTGATGTCCTGGTAGCCCGGCTCGGGATTTTCGATGTTCAACGCACCCAGACGGACGAAACTGGGTGTGCTATCGTCACTATTGACGCAGTGAGCCGCTGTGAGGACGAATCGACTGGCGATGAGCGATCCACCACATCGGAACGCTGCGCTCCCGAAGGAGTTGTACGCAATGGCCGCCATGTGGGGATAGACGCCCCTATCCACCCGTTCGCCGTCCAGAATGTGCACCGTCAGGGGCTTTCCACCGGATCGGATCTTCTCACACGCTATCATCAGATGATAGGAAATTTAATATATATTGTATATAATATATCTCCAAAGATATTTTCATATAAAATATTCCAAAGAATGCGCAACTCACCTGCCACCGACGGTCGCTCCTTCTCGGGAAGATTGACGCGGGAGGGATTGGGATTGGGCGTCGTTGGCCTGCGAAAGACAAGGTATATCAGCGATTAAAATTGGGCTCACGATGGTTTCCACCTGAGACAGAGACTGCATGCG

CTGAGGCCAACTGATGTCCATGCCAAAAGAATGCTCAGACAAACCGCGTCTCGGCTGGATTGAAGATGCTGGGAGCTGGATCCGCGTCTAGATTATTGCTGACATCGTTCCAGGGTTCCTGGGGCTGGGTATCCTTGTCTCTGTCCTGGCCCCGAAATCGGTAGGGTTCAAAGGGATCGATCAATTGCGTGGGCGTGGGTGCGGGTGTGGGCGTGGGCGTGGTTTGCAACACCATGGGCACTTCTCGAGCAGGCTCAATCTGCATTCCCTGCTCCCGCAGCCGATCGTTGACCAAATGTATGAGGTTGTTATTATTGGGGCTTGGATTGGTATTGAAATTGGAATTATTCGGTCTTTGGGGTCGTGATCGAGGCGTGGTCAGTGGACGGTTCACAATCCGTGGCTCACGGCCCCACTCCGGTTCCATAAAAGATCTCTGGGTGCTTGCAATGGGTTGAGTCTTCGAAGGAGCTATGCCCCAAGCTCCAACGCTTTGGATGGGCAACTTCATGGGTTCGTGTGTGGGCAACTTCATTGACTCGTGAGTGGGCATCTTCATGGACTCGTGAGTGGGCGTCTTCATCGACTCCTGTGTGGGCAACTTCAAGGACTCGTGGGTTTTCTGCGGCTTTACTGTGGTGCTTAGCAGCTTGTTGAAGTCAAAGAAGTTCTGATTCTCGTCCAGCCGACTTTCCCGCTCCCTTTTCCCCTCGGTGGTCGTTGGAGGCAGTGGCTCCGGTTCTGGTGTTGGTTCCCGCTTGGGTTCGGGTAAACTGCTGCGCTCGGAGCTCGTAGCATGGAAT

 

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