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CRIMIC CR01707

Gene Cyp301a1

CG8587

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:12569061..12566185	49B4-49B5	FBti0212951	91279

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
long band	OK	GCTTGCTTTGTTGGCGACTGAACAAC	ATAGGGAGCTTTCAGCTCCAAAGTGG

Images

Construct Design Sequences

sgRNA sequence	CAAGTGAAACGGAGAGTGTTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AAATTGGACACCTCTGGCGT	GCAATTGCAGTTAGCTTGGAG	CTCTCCGTTTCACTTGACCCA	TCGGGGAGCTCTTGGTTTTC

 

Amplicon 5'

Amplicon 3'

AAATTGGACACCTCTGGCGTACAATAATGCGAACAAACTTTTTTCAATAAGTGGACTGCCGTTCACAGTGATTTTTATGTCATAAAGAAATATATAGCATGCAATAATTTCGACACTTTGGCGTATGATAAATATCAAGAGGATAACAGGATATTTAAATGCTTTACCAAGAAAACAAATAACTTGGAACAATAAGATATGGTAATTGAGTTTAGAATGTTCAGAACATCATCTAAAGAATGTATATGAAACAATTGTTTACATTTCAACAGTTTTGAAAGCAAACCGCCAAATTGAAAAGAGCACTTTCTCTTTTAAAAACAGCCAAGAGAGCCAGCTGAAGACCTTCCGAACGTAGCTTGCTTGGATCGGCGAAGCTTGGGCCGCCAGTAGTTTGCACCAGGCAGCCGTTGAGTGTGGAACTTGAGTGCGGTTCCCGCTAGAAGCCAGTTTCGTTTCCCCGCAGATATCAGTGTGTCAACGTCAACAGAAGACAATTGGAAATCGAGGCTTACGACCAGTGAAATCTGATTTGAATTTAAATCGGTTTGGACGAGTAATGAACAATCTGTCGCTGAAAGCTTGGCGTTCCACAGTCTCCTGTGGCCCCAATCTTCGACAATGTGTGCCGAGGATCAGTGGAGCAGGTTCCAGGCGAGCGCAGTGCCGCGAAAGCTCCACGGGCGTGGCCACTTGTCCCCATCTGGCGGATTCGGAGGAGGCCAGTGCACCGCGAATACACTCCACTTCCGAGTGGCAAAACGCTCTGCCCTACAATCAAATACCCGGACCTAAGCCCATCCCCATTCTGGGCAATACATGGAGGTAACCGAAAACCAAACCAAACCAAAACCCATCATCAGAAAAACGAAAGACTCAAGACACAAACTCGCTCGCTTTCTCCAAGCTAACTGCAATTGC

CTCTCCGTTTCACTTGACCCAATTAACTTGGCCAGCTTGTTCAACACTTATTGAATTAGCCATGATGATGTTGTTCGTTTGTTTACTTGGATTTTGTGCGAAAAACACGCAACGTCGTCTAGTTTTTAGCCCACCTCGTAAAAATGATGTATGCTAACTAAATATTTATGTGGGACTTTGGTTTCCCTAACGATTCTTGTTAAATGTGCGTGTGGGATTCCATCAATTTACACCTGTCCAATTCCAAACCTTAAGTTACAATCCGTTCGATGGCACGTCATCCAGTTAATTAGCGTTCAATTAGCGCTAAATTCAAGGTCGCCCTAATAACGTGTGAAATTTTGCTGCATGCTGACGAATTTTGCTCACAAATCATATTTTTTCGCCATTGGAAAAAGTCAATGCATCTTTCATTGTCGTAGATAGTTTGTTTAACCTTGACTATTCCATAGACTGATGCCAATAATTGGTCAATACACCATCTCTGATGTGGCCAAAATATCATCGCTGCTGCACGATCGTTATGGTAGAATTGTGCGTTTTGGAGGTCTTATAGGACGGCCCGATCTTTTGTTCATCTACGATGCCGATGAAATTGAAAAGGTAAGCTTTAAGACACGCCAGCGGCTTGGGACTTTCCGCATGACCAACGAACCACGAAGGCACCAAGCCATTACACAAATTTATGGCACCGTACAAATTGCCCACAGTTGTCGGATATTAAGCTATAGCAATTTTATGAATTTCCAGTGCTATCGTAGCGAGGGACCCACCCCGTTCCGTCCCTCGATGCCCAGTTTGGTCAAGTACAAGAGTGTGGTGCGCAAGGATTTCTTTGGGGATCTGGGCGGCGTAGTCGGAGTGTAGGTGGAATTGCCTCATTTGTTGAGCTTTTAATTTATTCTGCCTTAATTCAGACATGGAGAGCCTTGGCGGGAGTTCCGTTCGCGTGTCCAGAAACCGGTGCTGCAGTTGTCCACCATTCGCCGGTATCTACAGCCGCTGGAGGTTATCACCGAGGACTTCCTGGTGCGCTGCGAAAACCTGTTGGATGAAAACCAAGAGCTCCCCGA

 

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