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CRIMIC CR01709

Gene CG7154

CG7154

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:7782865..7786061	28C3		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	long band	CGACCATGAAGTGTAAGCTGTTGTGC	CCAACTTGGAGTAGACGTTTAGCTGC

Images

Construct Design Sequences

sgRNA sequence	TAGGCATGCTCAAAAACAAGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGATCGACACACCATAGCGA	CGGCAAAATGTGTGAGCGAG	GTTTTTGAGCATGCCTACACT	GGGCATTAAGGGAGTTGGCT

 

Amplicon 5'

Amplicon 3'

CGATCGACACACCATAGCGATGGATATCCAGCGAATGCGTGCCGATCTGGACAGGATTCGCAACAAGCTGGACACCTCGCTGCAGAGCCTCAACTCGACGCGCACCCTATTCAGTGCCGCCAACAAGGCGAGTCTCAGGAAGTCGACCGCCCTGCAGAAGCCGACGCAAAACATGGTGACCTCCACCAATCGCCGCTCGGGTGGCAAACGAAAGGCCCGGACCGTTGCCATTCCGATGAACCGGCAAAGTGTGCTGACCAACAAGGCCCCTATTCTCAAACGAAAAGTTCGGTGAAATGCAAAGTAAAAAATGAGAAAAAAAAATGATTTAAACTTCGGCAAAAAAAAAATTATTTTCAAAATAAGGCATAAACTACAATAATTGTTCTTTTCAGTAAAGTATTCTTTCACTTTACAAGCATAAGTACATAGAAAAATTTACTGATAAAATCTGTTACTGTTTAAATCTTTGACATTATAAAATAAATTTAAATTAACAAATTTACAATTTAATTAACAATTTTTTAGTTTAAATTCAAAGCCGTAAAAATCGATATGTTATCAATTGCTTTTAGTGTTGGAAAGCAAATGGCTAATATCAGCGAGACGTCTTACAATTCTCTGGACAAAAGGAACGGTCACACTGCTCATGGCGTCTGTTTTTCGCCAGCGGATTTTTGTTTTTAATTATTTTGCTTACCATTTTCACCGATTTGCCTAATTGTTCGACAACTGGGAGCCTAAGATGGGCTCCTCCAAGAAGCACAAGAAAAACAAGTCGGAGCGACGCGAGAAATATGAAGGTGAGTGAAATGAAAGTGCTGTGTCTACGTGAGCCCCTGTTGCCATAGGCCATCTCGCTCACACATTTTGCCG

GTTTTTGAGCATGCCTACACTTAGAAAAAAATGGGTGTACATTATTTGTCACAATTAATACAAAATGCATGGTTAGAAATAATCTACGCGTCTAAAGGCAGTAATAATACTATTCAGCACCCATTATAATACCTATACCTTATATAACCTGGCTTTCTCCTATATATATTATAGAATACAGTCAGCACCAGGACCCGGCTCAGCTGCAACGAGGTCTAAAGCTTATCCTTAAGGTGGGCTCGAATGCCACGCCGGAGTACAGTGCCAATTCGCCAATGGTGGACGGAGGACCGCCCACAGCAGCCGAGGCCATGATGTCGCCAGTCCCCGAGGAACTTCAAGATCACCAGGGTCATCGCGAGCGCCACAAAAAGTCAAAGAAAAAAAAGAAGAAGAAGGATCGCGAGAAGAAGCACAAACACCACAAGGAGAAACGGCACAGGAGCAGAGATAGACATCGGGACGCTGGATCGGACGAAGATATGATGGCCGGCGCTGACGATGCCGCTTGCAGTGGATTCGCCCCATCGTCTGTCGCTCCACCGGCCGCCGATCCCGATTCCAGCCAAGATGGATTTAGCTTTATGGACGATGATCAATCACAACCGCTGCCAGAGAATATTTTGTTCTTCGCCGGCATCACCACGGATAACTCGCCATCAAACTGCCCCGTAACAAAGCCAATAGCTCCACGCAAGCTGGATGATATTCTGATGGGCTCTTCGCCCAACTCGTCGTCGTTGCAATCTTCCTCCCTGGGTTTAATAGGCAGCAGTCCCACCAAGCCACTGCCCGACGTAAGTTACATTACCATATGGCCAAGTTCATTACTACATTCAATGCATTTTAGCTTTTAATACCTTCACCGTCGACGCCAGGAGGAGCCAACTCCCTTAATGCCC

 

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