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CRIMIC CR01710

Gene CG4266

CG4266

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:21165986..21165992	57C6	FBti0210481	86494

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAGAGCTCGCACGACATTGACTACCA	GTATCTTCGTCGAACATACCACCCTC

Images

Construct Design Sequences

sgRNA sequence	CGGGATTAGTGGCTGGCCACAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCGCCATATTCGCCAAAGGT	GCCACTAATCCCGAATCCTCT	ACAGGGCGAATACGTTATTATCG	GGAGAACCAGAAGCATCGCT

 

Amplicon 5'

Amplicon 3'

TCGCCATATTCGCCAAAGGTATCGGACAGCTCCTCCTGGTAAACAAGCTTAGACAGGTGGCCCACCCACAGCGTAGTGCTGCATACTGAAAGCAAAAGCATTGGAATGCCGCATTAGTTTTTGGTGTCTGAACATGTTGTCAATATGCCCATTTGCCTTGTTGAGGTGTGAGTGCCACTGTTGGGATAGTGTTTAGAATTCCTTATCCTTAAGTATATTTACCAAGATTTAAACTATGATCTATGTACTTCATTAAGAAGCCAGTTCTTGGTAATTAAGCTTAAGTATATATTAGTGTAGAACACAACGTTTGTTTGCATCCTTATGTATATGTATGTAAATCGGGTCTCAAAAAATATTGCTCGAAAACGCATATTATATAGGGGGATATTCTAAGATCAAGAACCACTAAAGCTAAACACACACACGTCAACCGAGCCAAAGGAGCTGCAGAGAAAAGGAAGTAAATCTTTAGGATTATCAAAAACAAAAACAAAAAAAAAAAAGAAGGAAAAACAGGAAAGCACACAATGCAAATGGATCTTAAGAAGCACCATCTTGTGGCCTTTAAGATCCATTTATCATTCCCGTCATAGTTACCACTGAGATGCTCCTTTTTGATGTCGGGCAATCCCTTCTTGCGCCGCTCACGCTCGTGTTCCCGATCGCGTTCCTTGTCTCGATTGCTACGATCCATGCGATCCCGGTCCCTTGATCCGCGTCGCCGGCTGGACCGTGGTGATCGGCTGCGCGATCGGGAACGAGTGCCACGCCGACGTCGATCGTTGCCCGTGCCTCCTCCGGCGCCACGTCCTCGCGGAGATCTCGTCCGGCTGCGACTGTTCCGGCGACTGCGCTTGTAGCGATCACGGTCGGGCGTCGGGCTGCGGGAATCGCCACCATCCAGATTGATCACCTATCGGAGATGGGAGTGTTTGCAAGAAATTTGAGAGTGATACGTGATAAAAATTTTGTTAAGAATAAAAAAAAAAAGTTCAGTGGGATAGAGAGGATTCGGGATTAGTGGC

ACAGGGCGAATACGTTATTATCGACTCAATAAACTGCATTCTTTGGCGTGTGTTGACGTGTGGTTCTGTTTCGCTTTCGACTTTCATTCATCTAGTTATTTCGGACTAATTGGCAATTTGCGTTTTCGAGCAACATTTTTATTTACATATTAGGAACTTACCAAACGTTGTTCTAGCATCGGGTAACGAGTTGTTCCGCCGGATATTATGTGGATTTTAAAACCTTACCTTTGGCTTCATTTGCATATTTTTTAAATATTTTACTATTGGTTCGACTTATTCAAATATTGGCAATTGTTTTATAGTTCAAATATATGTTGGGGACATATAGATCGACTTATGAAACTGATTTAGGGTTTGCCCAAAAGCATCATCTTTTGATTTTAAACATTTTTTTTACAACAAAATAAAATTTAATTTTAAATGGATTGGGAATTGAATTGTTTGCATATTCAAGATATATAGTATTAAAGTTATTGATTAAATAGTGCAAGACCACCCCGTTACAACTAGTTGAATCAATTTGGATTCGAACACGCTAATATCCCATCAGCTATAACAACATATTGAACACATTTCCAGGATTAACTGCACGTCAGCACAAGGCAAATAGTATCACCCGTTTTACATCTAATATGTTTATACTTTAGACCAAAATTAATGGCGAAGTCTTGGTGAAAAGCATACACAAGTCGGACCCCAGGAACTAAGATAGATTGGAAAAGTAGAAGGGTATACCTCTATGGTCTGTTGCTCCGATATGAACTCGACATCCTTGTTAAGGTCCATGCTGTCGTTCATGTTCATGCCCATGCCCATGCCCGGCGGCATACCAGCATTTCCCTGAAAGCAAATCATAATGAAAATATGTACATCGTCATGAAATCATAAGATGTACCTTCATTACGTTCTGGAAATGCTGCTGCAGTGCCTCGTCCTGATAGCGATGCTTCTGGTTCTCC

 

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