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CRIMIC CR01711

Gene Hen1

CG12367

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:12146169..12146175	48E2	FBti0210482	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCAGTGTCCTTTCGCATTTGATGGCT	GCAGACGAAGACGATGAAGAGGAGAA

Images

Construct Design Sequences

sgRNA sequence	GTACCTGGCTGCATGAAATGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTGCGTGATTCCCCTACCAC	GCAGCCAGGTACATACTCTCC	ATGTGGGTGAAACGAGTCGT	GCACATCTCGGAAGCAAACC

 

Amplicon 5'

Amplicon 3'

TTGCGTGATTCCCCTACCACAAAAGCTACACTGGTTTGAATTTACTACCTAGCATTTGAAAAAAAGATCGCCAATTCAAATAGCAAGATATTTACATATATTGCTTAAATTGAGTTGCCGCATTTAATTTGAAGATTTCAATGCATCAATTTTCAAAACAGGAACTATCGAAAATATCGAATTGGATTCGTATTCTAGAAAGGCTTTAAAACCATTTGAAAATAGTTATGGTTAGGTATTTATGATATGATAGTTCCTACAGAAAAAAGGACAAATGAATTCGTTACACAAAAATGACCGCCCCTTTTTTTGAAAACAGCACCTATCGATATACAGATAACAAAGTTCTGGGTAGTTTTCCAAGTGCAGTGACAGTAAGATTTTCGTCACGGATTCCAGTGGTCACTCTAACGATACGGGCAATGTTTTCGCACAAGTTTATTTGCGGGTCCTTGACCAAAATGACTGAAACCGGCATTACATTCGATCCGCCGGTGTACGAGCAGCGATATTGCGCCACAATCCAAATTCTGGAGGACGCCCGCTGGAAGGATCAGATTAAGAAGGTCGTGGAGTTCGGTTGTGCCGAGATGCGCTTCTTCCAGCTAATGCGTCGCATTGAGACCATAGAACATATTGGACTGGTAAGAGCTTGACTTCGACTGGTAATGAAACAGGCACAAGTTGATTGTTAAGGAAATACATTTCTAGTTTATTTATTTCCGCTGCTGTTTATACACGCATATCCTAGCTTAGATTTGTATTGTTTTTATGAAATAACCATTATCTAGTTATATTAAATACTTTTTGAGGAGAGTATGTACCTGGCTGC

ATGTGGGTGAAACGAGTCGTACATGTGGTCTATAATATGGTTGTTGATAGCCTAACGAAACTAACACAAAACTCGAAAATAAATGTAATATATAAAAAATCATGGATTTCCTATCAAGGCCAATGGCGCACTGCTCCTGTGCACCTGCTAAACATATAAACATCTCTTACATCTGGGGACTACTCGTACATGGTATAATGGATAATTCAACAACATCTTCCCTATGGAGCTGAGAGTGCCAATCAAAAAACCTAATGTCAACTTATCGATAAAATGTTTAAAAACCAAAATTACACAACATGTTGTCGGTCTCACTAGTTCGTGTCCATGCCTCGCCTGCCGACAGGTCCGAAGTATGATTATAAGTAGGATCTGTCCTGTTCGTTCCTTAATTACCACTAAAAATACTTGAAAGCGAATTTAGATTTATTCTTTTCCGTTCCGTTCTGTTCTGTTCTGTTTCCATTTATCGTCCGACTTCCTAATGATCCGTCTATTAGTCCCTATTTCTAAGTGTCATCTGGCAATTATTTGCGACAGTGTCTGGACGAGTGGACACTCCGCCCCTTGCCGTAGTTGTTCTCGTCATCGTATTCGCCGTACAGGTTCTGCACAACGAGCAGACTCGAGTTATTCTCCGCATCGTGCTCGCCGTGTTTGGGTTCAGTCTTATAAAGAGCTCTGCGCGTACGGCCTGACTTCAGCTTTCGCTCTCCCTCGCCGGTGGCCGATGCCAGAGTGGAATTCTCCTTGCGTGTGGCGCAGCCACGTTTGTTACTGGCCACTGCCGATAGCGGCGTAGTGCGGTTCTTGCTAACGGCTCCGGCGGCCAGTTTGACGACGCCCGAGTTCTTGCCCGGACGACCCACTCCGCGATGGACCTTCACCGGCGGCTGGTTTTGGTGGGGCTGGGCCTGCCTGCTGGTGGTTTGCTTCCGAGATGTGC

 

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