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CRIMIC CR01714

Gene Ssk

CG6981

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:20189779..20188522	77A1	FBti0212952	91280

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CACGAGGTGCCAAATGTGCAGTATAG	GAGGTTCGTCTTGAAGCGTCATCGAT

Images

Construct Design Sequences

sgRNA sequence	ATACGCCGAGTTGTACGGCAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCCACCTACAGCCACGATTA	CGTACAACTCGGCGTATGAG	GCGTGGCCAAGAAGTGAT	CCGTGGCGTGGTCTTAACTA

 

Amplicon 5'

Amplicon 3'

GCCACCTACAGCCACGATTATTGTGCTAGGGTTGTGATAATTGAAATGCAGATTAGGTTCCAGTGACTTTTTGTGTGTCTGAGACTTTTTAATCAGTAGCTGGAATCCACTGGGCTATCGATCATCGGAATCGTGACAAGGTCAGTGAATTTTTAATCTGTTCGAGGGATGCACCACCGGGAGCTTTATCAGTGGGAATGGGAATGGGTCACCACGAAAGTATTTCAGAAATACATCGATGACGCAAGAATAATTGTTTCCGGTCTCTCAGAGCCGCGGGTATGTTTCCCCGTTTTGTGGCTTTGTGGCCGCCATGAATAATGTGTAACAATGCTGTGGAAAGCGGCAAATCGGAGCACCTGATATATCGCGATGTAAGGCGACAGAGCTGCGATAAGGCCGTTTTGATAAGATACGCGGGTGGGGCTGGCGCATGCGCATCTGGGTTTCATTGGGCTTCGGAGCCTGAACATGTTGAACCGTCAACGAATTAGTAACCCATTTCGAACGGCACCTGTTATCGTGAGCCCTATCTTATCTTATCGCTGACCGTGTATGCTCTGATTGCAAAAGCTACAAATTCTTGGCGACACGACGAACTGGGCGCGACAGGTTGGTTGGTTGCCTTTCAGTGATATTTTTCAGTTTAGTCGACGAACGACAACGATACGAAAACGAATCATTTCGCTGTAGTTTCCGCGTGAAAATTACAGAAAAGATTGTGAGTGCCCCTTGACTGGGAGAAACCCCTCTGTGAACTAAAGCATTTGCCCAAATTCCAGCTCAGCGATAAGAGCAATCAGCATATCTAGTGCGAAATGGTGTCCGTGGAGACTGTAGGCTCTATTTTCATCAAGGCCCTGAAGCTGGTAAGTTTTGGGCTTCTTTGGTCCCTTCCTATTGAGCAATAGCCTGTTTGTGGTCGCAATAACTCGGCTGATAGATAAGCGCGCGTTTTATCAGTCCTGGCGTATTAGCAATGTTACTCATACGCCGAGTTGTACG

GCGTGGCCAAGAAGTGATTCATTCATTCCCATTCCCAATTGTGACCTCATTTAATTGCTCTCCTGTGCGCTCCTTTTTCGCAGATCATCAATCTGGTGATCATCTTCTTGTACCGTTGGGGCGATGGTGGCGAATTCCTGGGCATCGGCGGCACGTGGAACTTAAACGAGGAGAAGAGCGCCGATGCGGAAATCGTAGCCTCAGGCGTCATGGTTGGATTCTTGATTTACACTGGATGCCACACCATTGCCTTTGCCTTCGGCACAACCAAACATAAGGGGTATGTTGAATGCCGATATTGATTATAGCTGAACTTTGACCCGCATCTTATTCCTTTTCTTGTATTTTCCGCCCTTAATTCCACACTCTCAACTCACAACTGTTTGTTTTCAATGGCTTTCCGTTTTTCAGAGAGCTGTGCGACACCATCATGAACGTGGTTGGCTGCATCATGTGGATCGCCGTGGGCGGAGTGGCACTCCACTACTGGAAGGGTTACATGTCCGACGAGGGATTCCTGTATGTGAACTCGGAGCGTCAAGTGGGCATTGCCATGGGCTCGCTGTGCGTTATCGAGGGCGCACTGTACCTGCTGGACACCGTGTTGGCTTGCATACACTATTCGAAGGGCGACACCGACTACACGCAGTAGAAACTGAATAGTGGGGATACCAATCGCAGCCGCCGAATAAACACCATCCACATTTGTCTTTATACATAGATCGATTTAAGTGCAAATTAGTTATTATCCGCAATCAATTATCAACTATACGTTCGTAAGGGTCAATTGTCGCTGTACTATACACTAACTGTTTACAATTTTCATTCACGTTTAACATCCTTAAATTGGCAAATACAAATAGTTAAGACCACGCCACGG

 

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