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CRIMIC CR01715

Gene MED24

CG7999

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:8196573..8191270	66B11-66B12	FBti0212953	91281

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACGTTGTCAGCGATGATTCTTCCTCG	GATTGCTGCAGGAAGACGTCACATTG

Images

Construct Design Sequences

sgRNA sequence	CACCCAATCCCACACCCAGGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CAGCTAGCGACACGATGAGT	GGTGGTGCTCAATGGCAAG	TGTGGGATTGGGTGCCCG	CAAGCGCTATCATCCCGACT

 

Amplicon 5'

Amplicon 3'

CAGCTAGCGACACGATGAGTGAAATGAAATTCAAAAAATCGGAGCGTGCAATGGAGGAACAATTTTCGGCATATATAAATAGAATATATTTTGTACAAAGTCAAATATTTTAGAACATAATGTTTAAAATGAAAACAGTTTACAAATGAAACTCATACGCTACTTTATTGTATTCAATAAATTATTTGAATTAAAATTTGTTTGTGGGTGCCTTTTCGGTGTCTTAAAATATATTTTAATATTATTTCCTTTATTTGGCTAAATTCCTCGTTGCAAAAACAAAATTTTCAAAATATTTAAAATTGGCGACAAATTTTAAGATTGTGTGTTTTTCAACACTGTAGGATGGGAAAATTATGGTACTCTTTTGGTATGATTAATAAAAGATACCTCGGTCACACTGAGCGCTGCTAAAAAATATATTAGGTTTAAATAAAATAAATTGCGTGAAATACGAGAAAAGTAAAAGTTATATTACTTCCACGGACGCGAGAGATGAAGGAGAACAACAAGATCCTCCAACTCATCTATGTGGCGTGGCGCGAGCGCTGGACGGACTCCCAGTGGGGCATCAACATAAAAAAGGCAAGGCAACCCAAGCAAATCAACACACCAAACAATCAATGTTCGTGTTTACCAATATATTGTAAACGCAATGAGGCCGGTACATTGAGAATTTTAGTGACTCAAATTGCCCGCCTCCGTGCTCTTAGTCTTGGGCTCGAAAACCGGATCCTCTGCCTGGGACAGTGCCAAAACCAGTTGGCGTTGCTTTACGGAGAGATTCCGGGGTATACGCACCTTCAGCGTGACGATGTGGTTGCCCACGCCCTCCCTGGAGCGCACCCCCTTGCCATTGAGCACCACC

TGTGGGATTGGGTGCCCGGCTCCACACGCAGCTCCACGCTTTCGTACAGACCCCGTATCTGAAAGGATCCGCCCAGAATGGCCTCCGAGATGTTTAGGTGCTTGTCGGTTAGAATGTCATTGCCAACGCGTCGGAAATAGTCGCTAGACGGCACCGACAAGCGGTAGGTGACCTGCTGCTTCGTTTCCGGATTTATAATGTTCACCACATCACCGTCCCGGGATCCGGAAGGCACCGATACCATCACATCCACATTGGACACCACGAAGCCGCGATTCGAACATGTTTCGCAGTCGTTGCGATTTGTGAATCGCTTGCCCTTGCACTGCGTGCATGTGTTCACCGATGAGAAGGTTGGCGTTTTGGTCATGACCTTTCCAGTTCCATTGCATCGCCGACAGGGTTCCTTGCCCACATCCCGATGCGCCATCAATTGTGACTTGCCCTTGCAGGTCTCGCATTTCCGCAAGTAGCGCAGCTCTATCCGCTTCTTGCATCCCACTGTCGCCTCAAGGAAGTCAAGTGGCAGATCGAACTCGTTGCTCTTCAGTTTGTTGATCTCTAGAAGGCGATTCAAGGATCGTGTTCATATCGTGATATAGTGAAAAGTGAAATGGTGTTTGATGTATCTAAAATCCGTCTGCTTACCATCATTTGTCGTCTTATCCGAATCGAACTTCTTGGCCTCCTCGAGGCCCACATTTAGGGGATTGCCCGCCTGTTCAAGAAAAGCGCGCTCATCCTTAATCCCGCCCAGCTGATCGTACTCCAAGCGCTTCGTCTCGTCGGTTAGGATGTTGTAGGCGTTGGACAGCTCCTGGAAGTGCTTCAGCTTTTGTTCCGAGTGCGTCGAGTCGGGATGATAGCGCTTG

 

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