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CRIMIC CR01720

Gene CG13675

CG13675

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:8165518..8160315	66B10	FBti0212954	91282

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	short band	CTCACGTCTTAGCCAGAAACACACAG	GATTGGGGATTGGTACTTGGTGTGGT

Images

Construct Design Sequences

sgRNA sequence	GGCTGGAACCAATGGATGCTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCGCAGTTCACATTCGACCA	GGTTCCCCAGTGAGATCGAC	TCATTTGAGCGCCAGTCTGT	CTGGCTTAGCGGCTTGGTAT

 

Amplicon 5'

Amplicon 3'

TCGCAGTTCACATTCGACCACCAGTCGCAGACCCTGACGGCCTGGTTGAATACGGTTCCATTTGGGCAGAGGAAGGAGTACTGATGGCCCGAGTGGAGGCACCAATGCCAGACCTATGATGAATTCCATGTGGTTTATATATAATTTCACCTATTTACAGAGTCTTATTCGAACTCACCTGACAACGCGTCTCTGTGTCCGCATAGTAACCCGGCTGGCGACCCTGGCAATTGAAGGCCAGACCCTTTGGCACGGCGTCGTAGGCGGGGTAATCCTCACCAGCTGTGTAGCCATCGATTTGCTAACAAAAGTGAAAATGAGATAAATTATTCAGACAGTTTTGGCAAATAAATATTTACATCGTAATTAGGTTACGAGTGGGACTCTTCCCACCTTTAGCCCCATTTCGAAACACTTAAAATGCAGTAAATTGAATTAAATAATAATGCTGTTAAAAGCAAATTCCCTCGCACACCAGATATCGTATTAAATTATGACTTTGTAGATCGCTCGTGCGAATTATCTCAGCGCAGGCAGCGCAGCGACCTTCAATTGTGGGTGGCAGCATGGGTGGGAGGTGGTGGCACATGTGGCTGGCAAACATTGCCCAAGCGCAGCAACAAAATGAACTCTAAACAATAACAAATTACAATTTGAAACAATTGCCACACAAACAGAAATTTAAAATGCAAATGCAATAGCCAAAAAAAAAGGCTGTCGAAAAAAACCAACAAAAGAACCAGCGAGTGTTGAAAATTGTGCACTTGCAACAGTTGCAAGTAATTGGGGGCGCAAACAGAGGGGGGGCACCATGTGACAACCGACTGTCAATCTCATGCGTACGAAACCATGAAATGCCGAAAAGAGCGAAATAAAACAATATAACTCCGGCGACTTCAACACGGATCACAACACACACCGCGTATCGAAGCTGGAAGAGATGTGGATGTGATTCTTTTGTGTCGATCTCACTGGGGAACC

TCATTTGAGCGCCAGTCTGTTGCATCTTCGCTGTCTGTCTATCTTGATCTTGCCTCGATTTCTTATCCCATTGTTGCCGCTGTTACGCTCGAATAAATCTCTCTCCCTTTTCAGGGGGTTAAAGTTGCCGCCGTTGGCGCTTGTCTATTTGGGTTAATGCTGAGTTTATGCTAATTGAATACGTTGGGACAGGGCAAAGTGATTGGCAATGTGCAGCACATTGATGGGGTGGGAAAATGGCCGAGCACAAGGAGGTTTCTTCATGGGAAAAGTTTGGGACTTTATGGCCAATTCGCAAACATCTTAATCAAGGATCTTATGCAGCTTTAAGGCGAAATAGTTATTGCTTTATGTGCAAAATTTTTAACTTAAAAGTAATTGTTGTTATATTTTCAGTCACCTGGTTAAAAAATACCAAATGAAGTCGTTACCATTTTTGCTATCTTCTTTAGAAAAATTTACATCAACAAAAACCTTATTAAACAAGCAAACACTTATTTTCCAGAGTGCAATTGCAACCAGGATGCACCTTCCACTCCAGTCCCATTTCGATTGCATTGCAAATTCGAGCTGCCTCGTTTTTCGTCTTAACTTTTCTCAATCACTTGCAATTTTCACGCTAATAACTTTCCAGGGGCTTTTAGCATTTCCAGCGGCAACAAAAGACTTAAACAACAAACTATATGGACCGCCCATTGCCTTACTAACCCAAAAAGCGGACAAGATGCCACTCTCGACAAAAGCCCCGCACACCATCAATTATTTCCCTCCACTCTCAACCTTTGGCCAGAGACCAGACCATTTTCCTTTAAATACCAAGCCGCTAAGCCAG

 

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