• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR01721

Gene LanB2

CG3322

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:9623980..9614914	67B1-67C2	FBti0212955	91283

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACTGGGATCCACGATATCGGTATCAC	TGATTGCACCCACCTTTGCACTCGTT

Images

Construct Design Sequences

sgRNA sequence	GTAGGAAGGAAAGAAATCGGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AAAGATACGCATGGTGGGCA	CGAAACTGTCGCTTAATTACTCA	CGGGGGAAGTTCTGGGAATC	GCAATCTCACCGTCCCTCAA

 

Amplicon 5'

Amplicon 3'

AAAGATACGCATGGTGGGCAGTACAAGCACATATATATGTACTTCACTCGACTGGAGCTCATGCTCGAGCGTTTATTTATGTCTCTGCTTGTTGTTTAAAATTTTCATTTGCAGTTCTCGTTTTGTTTACCCGAAAAGCAAATGAACTCATTGCCGGCCTGGCCACTAAAGTTTATTTTTCAAGCGATTTGCTCGGCGAACTAATTAAAAATCGGCCCCGGAACATGGAGCAATATGTGGCACACATGTTCTGCGATTTGGTTATATCTAAACTGATTCATAATCTCGTGCATTTTCCAGCATATGAAGTCGCTCAGGCACACCAACAAGAATAGGATTTCGTAATGAATTGACGAGCGAGACACCAAAGTACAACCTCGAAGGAACAACCCACCAGCCCAGAGAGCCACACAGACTTTGGAATTTCTCCACTCGTCCTAGTGACACCCAAGCCCACCTGTAGCCCAACGAGCCACCCAAAACCGAACCCACCATGAAGCGCAGCCGGTGGAGCCACAGTGGCTCCTCCACGGCTCGACTCCTGCTGATCGGAGTATTGTTCGCCAGCTGTTCCACCGCCATCCTGGGTGCCCAGCGTCCCCCCATCAACTCCGCCGGAGGTCACGAACTGCGCGGAACCACCTTCATGCCGGCCCTGGAGTGCTACGATCCATACGGCAGGCCACAGGTAAGGGACTAGCACTCGGAGCCACTGTTTACCCTCATTGTTTGTCCACAAACCAATTGAGAAGAATTGAGAATTAGTGGTCGGGCATTCAGCTTTTGGGCTGGGTCAATTCGAGTGAGAGATGCCAACTAATTGAGTAATTAAGCGACAGTTTCG

CGGGGGAAGTTCTGGGAATCTATCAATTACAGTGGTCAAAACAGAATAATTTTAATGAATAGTATTGTTCTTTCTTTAGTAAACTTTCAGTTGTACGAGTAATTACATATATATTTTAATTTGTAGGTATTAAATTATTATTATTATTGCTTTTTTATTCCTTAGAAATGTCTGCCAGAATTTATCAATGCTGCCTATCAACTGCAAATTGAGTCAACTAATACCTGTGGTGAGCAGAATGACAACCACTTCTGCATACAAACCATGAACCAAAATCACAAAAACTGCGAATTTTGCAAGTTAGTAAAGAAAAAGTCCTACAATTCCTTCATAAATAATTAATTTAAAAAATTTGTTTATAGATACAATGATCATAATCCATCCTTCTTGACGGATTTGCATGATCCGCAAAGTCCAACGTGGTGGCAATCGGAGACCATGTTCGAGGGCATTCAGCACCCGAACTATGTGAATCTGACTTTGCACCTTGGTAAGCATAATAGATGATGAGTTTAAAAACAATATTTTCAATATGTGTACATCTTCTGAAACCCAGGAAAATCCTATGACATCACCTACGTGCGCATTCTCTTCCGCTCACCAAGACCCGAATCCTTTACGATTTACAAGAGGACCTCGGAGAGTGGACCCTGGATTCCCTACCAGTTCTACAGTGCCACCTGTCGCGACACCTACTCCCTGCCAGATTCGCGAGCCATTCGCAAGGGTGAGGGCGAGGCCCATGCTCTGTGTACCAGCGAATACAGTGATATCTCGCCGTTGAGGGACGGTGAGATTGC

 

---

Search Again