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CRIMIC CR01722

Gene CG6083

CG6083

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:11624528..11626125	68C15

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGCCTGGTAGTTGGAGTCGAACATAG	CCCACCAACTCAAAGGCTACAATTCG

Images

Construct Design Sequences

sgRNA sequence	GTCAATGTCAAAGCTCTTCGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGAGCAACACCTACGCCTTC	CGGGGTCAAGCAAAAGCT	AGAGCTTTGACATTGACCGTG	TTCATCATGCCCAGACGCTT

 

Amplicon 5'

Amplicon 3'

AGAGCAACACCTACGCCTTCATGGTCATAAAACGCCCATTGCAGTCCAATTCGTTAATGGCCTGTATGTCATCGACGGCCAACTCAAAGTCCCAGATTCGCTTAAAGTTGTCCAACATGTGCTGTTTGCTTACTGATCTTGGGATGACAATTATACCCGATTGTGTCTGAAAGCGTAGGAGCACCTGTGCCGCAGTCCTTTCGTATTTCTCAGCTATAGCCAAGATGGTCGGATGCTGGAGCAGTGGATAAGCGCCGGGCTTCTCGTAGGGCGTATGTCCCGATCCCAGGCAGCTGTAGGCGGTCACAGCAATGGCATTGTCATAGCACAGCGTAATCAATGGCTTCTGACTAAGGTACGGGTGGCACTCGATTTGAAGAACTACCGGTTTAAGCTTGGCCACACTGAGAAGTCTATTCATCTGCTGCTCATTGAAGTTAGACACACCGATGGCCTGGCACAGACCCTCGTCCACCAGATTCTCCATTGCACGCCACGTGTCCACATAGTCGATGTCCTCGAATGCGGCCTTATTGGTGTCCGGACACGTGGGATATAGGTTGTCACTGCCCGACTTGTAGGCCATGGGCCAGTGCATCAGGTAGAGGTTTAGGTACTTGACTCCCAAGTTCCTTATGCTTGTCTCACATGCCGGGCGCACCAAATCCGGCTTGTGGTGTGTGTTCCAAAGTTTGCTCGTGATGAAGAGTTCATCTCTGCAGTGCAATATGTATTTATGGCTTAGATAAACGATTGATTAGGCCTGAAAGCAGCACTTACCGTGTGACCACTCCCTCGTCCATTTTCTCCCGGAGAGCAGCTCCCACTTGCGCCTCATTGCCATAGATATGAGCGCAGTCAAAATGGCGATAGCCTATATCAATAGCATCCTTTACTGCTTGGGTCACCACTTCCGGCGGGCTCTAAAATGGCGAATAAAACGTAAAAAGCTGAGGTGCATCAAAAGCTTTTGCTTGACCCCG

AGAGCTTTGACATTGACCGTGAAATATGCAGTGGAAATGCACATTTCAGCTGGCTATTATTTAATAACAATATATATCTATGTAAAAGTACTTACCCGCCAAGTTCCGAGACCCAACATAGGCATATTTTTACCATTGCTCAAGAGAAAATTCGGTGTCGACATGTTTGAATCCTTTTGCTCCTTGTGAAATATGTTGTTGCACTGGCTTCGTTTTTGGAAAGCTTTAATTTGTAATTTACTAAGTGAACTATACTCAAAGGCCTATTAAACTGGCTAGAAACAGCATGTCTTGTGACAGCTCTGCTATCACAACTATATGTACGAAAATTGTTTAAATTTAGAATCTTTTGGGCAAGCAAAAGACCTTCGCGGAATATAATCGATTCGCTGCAGAGGGAAATTTTAAAATTTAAAACAAAAAACTCTTTTTCTGCGTTCAGAGTTTTATTTTATAAACCGAGTATAATGTAAATAAATACCTTTTTTTAATAAAATAAAATAAAATGTTAAAAAAAAAACTTTTGAAAAAATATTCTTTGTATTATCGCGTTATAAAAAACAAAGACAATCGATAGAAGTTTCCGATGAAAACATCGCATTCGCAGATAGTGCCTGACCTACCATCTCTAATTTTGCCGCCTGCGAAAAACACAAACAATGTTCGGCACCGTAAATAACTATTTATCCGGCGTTTTGCACGCCGCCCAGGATTTAGATGGCGAGTCCTTGGCCACGTATCTCTCCTTACGAGACGTGCACGTTCAGAACCACAATTTGTACATTGCCCAGCCGGAAAAGTTGGTGGATCGATTCCTGAAACCGCCACTGGACGAAGTTGTGTCCGCCCACCTGAAGGTGCTGTATCACCTGGCACAGGAGCCTCCTGGTTACATGGAAGCCTACACCCAACAGTCGGCAGCCTGCGGAGCCGTTGTGAGGCTTTTGCAGCAGCTGAAGGACGAGAATTGGTGCCTGCCACTAATGTACCGCGTGTGCCTGGATCTAAGGTACTTGGCGCAGGCCTGCGAGAAGCATTGTCAAGGATTCACACCTGGCCACGTATTGGAGAAGGCTGCCGACTGCATAATGGCCTGTTTCCGCGTTTGTGCCGCTGATGGACGAGCTTCAGAGGAGGACACCAAGCGTCTGGGCATGATGAA

 

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