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CRIMIC CR01723

Gene not

CG4166

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:18622129..18618305	75D4	FBti0212956	91284

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCTGCTTGCTGTCGATAACACATCAG	CCCGTGAAGATCTGGTCAATGATGCA

Images

Construct Design Sequences

sgRNA sequence	GTGACCTTTCACGTGACTCACGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGGGAGCCGGAATAGAACTC	AGGTCACTGACACCCCGG	CGGCGCACCTCTGATTGTAA	TATGTGTTTGCGTGCAGTGC

 

Amplicon 5'

Amplicon 3'

CGGGAGCCGGAATAGAACTCCTACAAAGGGGGATAGCATATCGAATAGAAAAGACTCCTCATAAACACCACAGCATATTGAACTCACCTGGAAGAGACGTGACACTTCACAAACGAGACATTTGTGCGATGACTTGCTGCCGCAGTCGTGACGATCTGACATAAAGTAATCGCTGAGCAGGGGCGTGTGAACCAGAGCCTAGAGGAGACAGGAGTAAGTCAGTGAGAGGAGACTCGCCAGTTTAATTGGATTTGCCCACCTGGACGATGCAGTTCATGAAGCAAGTGGCGCCCAGATTAAGCAGACCCCTCAGTCCGATCGTTTGATTGGGCCTCACCAGCCGACGCCTCGCATTGGCCAGCAGCAGATTGGTCTCCTTGGTTGTGGGAACCCACGGCACCCAGCCGATGCTCTTCTGCAGATCCTTCGCCTCCAGCTTGCGGTTGATCAGCGCATACTCGCGACTCCGCGCGTCGTAGATGAAATCTCTGCAGGCGTAGCAGTAGAGCGTTCCGTGGGACAGCTCCAGGGCCACATTGTGCTTCTTGGATCGCAGGTGACTCGTGATGTGGGCCCCCCGACATCCGAAGTAGATGCAGTGCAGGCAAGCGTACAACTGGATGCCGTAGCTGCCGCACTCGAAGCAGTTGCAGTGAATAGCCTGGCGGAGGTAGATTTTAAAATGGCAGCTGATAAAAAATTATATTCGTTTTTTTTCTAATCCCATTAACTTTACAAAAAATACGTCCCATACTAAATGCATAAAAGTGTAACAAAAATTTGAAAACTCTAAATCTCGTAAGCAATATAATATATAATCAATATGAAGCTAAAAATACAAAAGCCATGTAACAATATTAAATATTTTGAATATATTTTTCTTGATATGTAATTTCACCGTAATGAAAACAAAACTTTTTCGATGCAAAAGTATTTTTCACAGTGCCAATTTGTCAGCTGTTAAAACAGCTGTTGCATGCTTTACCCATTCAAAAAAGTCCGCACTGCTTTGTGGTGAGAACGGTGCGTTGTCTGCCACTGTCATCCCACAGCCGGGGTGTCAGTGACCT

CGGCGCACCTCTGATTGTAATTGCGTTCTCCCCTTCGCACACTCGCACATTCTCACCTCACCACGCCAGTAGCGCACTCATTCTAGCTCCCACTCTCTTCCACGCTACTGAACTCACCTTTCGCTCGCGCGCATCTCTGTTGACGCAAGCAGCGAAGTAGGCGTCGATGACCCGGAATGTATCGTAGCTGTGCTCCTTCACATAGCTTTGATAGTGCCGACAACCCGTCTCGGACATGACGGCGCTGCTGGCGTCGGCGCTAGCGTTGGCTTGGGCGCTGGCGTCGCTGCCGGCGTCGGCGCTCTCAAGTTCAATAGCAAGTTTTTGCACGGTACGCAAGCAGTCCGATAGGCGCAAATGTTTGGTGGCATCTAGGGGGTTTTTCGCGTCCCTGTGGCCCTTTGCTACTCTGTCTCGCTGCTTGTCGCTGTTAACTGTTTTGATTAGATCGGCGGTGATAAAGCGACCGGCATTTGTTGTTGTCGCTGCTGCTGCTGCTACTGCTAGTGTTTCGTTCTCGGCCGTTCCCCGGCCCGCTTCTGCACCCACCGCCCACCGTTCCGTTCCCCTGCCGAAGCTTCGGCCACTGCTGCTGCTGCTGCTACTGCTAAATACGCTCGTATTACTATCAACACTATTCTCTTTTGTTTTCGCCCGTTTCGCCGACGACTGCAGCGGCACGAATGCTTTGTCACACTTGCGCTGTTTTCGCCGAATTTTGGCCACTTTTGCGGTTCTTCGCCGGGCGGCCCAATTTTGGAAGTAGTTGGGCTTTTTTTTTGTGAATTTCTGTGATTTTTCGCTTGCTTTTCTTGTTTCTATTTCGCACTGCACGCAAACACATA

 

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