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CRIMIC CR01724

Gene Msr-110

CG10596

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:5577348..5566919	64D3	FBti0212957	91285

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGCACACTTCCACTGACCCAATGCAA	TTCGTGTATGCACACACCGCATACAG

Images

Construct Design Sequences

sgRNA sequence	TAGTCACGGCTTCCCACTGCTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCGAACGATGCGAGCCATTA	TTGCGATGATCTAAGGCGCA	AGCCGTGACTAATGTGCACA	TAATGACTGCAGACCGAGCC

 

Amplicon 5'

Amplicon 3'

TCGAACGATGCGAGCCATTATTAGCCAACTCTATAATTTTTTAAAATTCAGTGCCCCATAGAGCACGATACTAATCTGCCATGGCAACGGATCATTGAAGCACAACGGATTGTACGGATTGGCATATCGGTTGGAATACATATTTCGCGAACTCACCTTGCCCACCTGCTGCAAAGTCTCAAGCATTAGCTCCTTTTCCAGCACCGAGTCGAGGGCCTGGACCTGATCGCACGAGGCCTTGGCCTGCAGGTAGGAGGAGGCCAGGATGAAGGATCCCAGGATGAAGGCTGACACGATGATGGTGAAGGCGGTGATCTTGGCGATTTTCACCGAATTCGCCTGGCGCTTGTAGGCCTAGAATGGTACAGAAAATCACATTAGCTTTGCGGTTTTTTTCAATGAACATACATAGTTTTTATTTGTGTTTGCAATTCAATATTTCTACGTGCTCATTTGCCTATGACCTCTCTGTGTTTCGGTTTTTTTTTTGTTGGGGGTAAGGTAAAAGCGACGACCTTACTCCACAAAATACACCTCAGCATGGTCAAGCGCGTTTTTCGCTTCAAAACGTCGAAGTTTTCGAGAATTTCAAAGAAGTGCTGGGTGCATAATTTGTGATTTTTTGTGAGTGATAATAACTTATTTACATAAAATGTGTAGGGGTTACTTCAGTTGCGACTGTTTTAGGATGGGTCATGCAATTTCATTAATATTTTATTATCAATGGGCTTTTAAGTGCTGTTTCCAGTTGACAGAGGTTGCTCGGCATTAATCATTAAAAAGCCAAACAGTCAGCTATAAGATGAGACCCAAGGTAATGAACAATAAGTGATTGTTAAAGGCATTTAAGTTTTTGGCAAGTGCTTTAATCGAGAAGAAACTGCGCCTTAGATCATCGCAA

AGCCGTGACTAATGTGCACAACAAATGCTTTCCGCGTAAGGTGCAAAAATTTACAAGTGGATGCAAAAAGCCGCAGCATAGGGAGTATCCACAAAAAAGTTAGGCCAGCCAAATGAATTCCGCATTTGAAGATTTTTTGTTGCGCAAGAGGAAACCCTCCGCAAAATTGAGGAAGTGGCAAGCCCAAGAGAAAAGGCAGTCTCGGGTGTGGGTGCAGTCAGGTGAGGCGGCTTTTTCTCGGTTATATCTATATCTTTTTTTTTTCTGAAATGTGCAATGTTTTTTGTTAACACCTCTACGTGCGTGGCTGGCGTGGCCCCAAAATGCAGTTGCGAAAGTGAATTGTGAGTGAGTGAGTGATCATCATCATCATCAACACCAGGCGAAGTGAAGTGGAAGTGAATGGCTCGCCTGGCACTCTATTTTACTCTACTCTACTCTACTCTACTTGATGAGGTTTTAATTTTTTTGCGTTTTGTGTTTTTTTAAACTTTTTTTGTGAAGATTTTCGTGCAGACAAAAGACTTGAGCGACAAACCTGATGATTAAAGTTAAAAAATCCCATCTGTGGTTGGTTTTGAGTGTTTGGTTCGTTTTTTGTGTGGTGGCGAGTGCGGGGGAATAGAGAAAATTTCATATTGTCAATCACGGTTTCGGGAATGGTTTGGGTTTCGGTTATTCGCATTCACATTTTTCAGCCAAACCAAAGAAAAACGATTTTTGTGGTTTTTGTTTCGAGTGTTGTGCACACACACACACACACACACACATTGCAAGGTAAAAATACATAAAAACAAGAAAAGACAAAAAGTTAATGAGATTGTACTGCAGTTGTGTGTGGGGTCGCTTAATAATACTTAAGGTGAAAACCTAACACATATGGCGCAAAGGTAAACAAATACTGCACAAAAGGGAAAGCTCTTGGCCAAGGGTCGATTGTGAAGGCTAAGACAGAGCTACTGACATATTAGGTAACTCATTGATAGATGAACTCTGTTATAGAAAAGGTCAGACTATTGGCTCTATAAAGTAATGGTTGTTATGCTCACTGTCCCATAATAGAACATCATAATAAAGGTAATTCTAGTTCACTTTTTGCATTCAGTACATATTAATGATAATTACTTTACAGATTTCAGCAGCACTGAGCATTATTCCTAATGGGTTTTTCTAATGGCTCGGTCTGCAGTCATTA

 

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