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CRIMIC CR01726

Gene CG13312

CG13312

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:8807213..8805811	66D15	FBti0212958	91286

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAGCATACTGGGAACACGAGGCACTT	CGGTACCACTGAAAGTAGCACTGAGA

Images

Construct Design Sequences

sgRNA sequence	TGAGAAAGAACAGGTGCTCCAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGCGTCTACGTAAATCCCGG	AGGATGCTGAGACGACTCTG	TCAAACATCCCATTTCCACCAAG	GAAAAGAGCAGGTGCCGTTG

 

Amplicon 5'

Amplicon 3'

TGCGTCTACGTAAATCCCGGCTACTGTCTTTAATGTTCAGCTCATTATAAATAAACCGTCAAATACTCTTTGCATAGAGATTCCTTTTTTAATCTAAAACCACTCCATTGAGGGTTAAACTGGCCACTGTGGTGGAGCATGTGGATGGTAAGGTGGAGACACATGTTCTGCTGGCACTGTTGTAGTAGAGGCTACCGGGACATGTGTACATATTGCCACCCCAAGTGCCTCCGTTCAGAAAACAGTCGATGTACTGATGACACGTGGTGCTGGCATCCGTACCCACCGGGTAGTAGCCCGCCGACTGCATCGCCTGGCAATATGCCTCCGGATTACTGGTCGTGGGTGCCGCAGTCGTCGTGGAACTCGTTGTTGCAGTTGTGGAACAGGTGGATGGCATGGTGGACACACACATCTCGGAGGCACTATCGAAGTACATGGATCCCGGACAAGTGTAGGTCTGGCCAATCCAACTTCCGTCAACCAAAGTGCAGTAGATATACTGGCGACAGGTCGTATACGCGTTATATCCGACCGCGTATTTTCCCTGCGACTGAACCGCCGCACAGTAAGTGGATGCCGAGCTGGTGGAAGGTGGTGCCGCTGTGGTGGAGCTGGTGGTGCTGCTCACCGTGGTTGTGGTTGAGTCATCGCTATACGAACAGGTCGGCATCACCTGCAGAGTTATGTAGATTTATTATTTATTGTGGGCCAGAGGAAAGGAATATACATATTCTGTCACGGAATGAACAGCGTTTTTCTTTGTCCTATTAGACATACAAGTAAATTTCCTAAAAACACCTTGGTTTCCTCAAAATCAATTATAATAATTCAAGCTTTAACGAAACACTTCAAATTGCATACAAAGAGCTTATTTTCTCCAGTCACTTGGTATCACATTCTAATGAATAGTGCTTCTTGAAAGTTTAATTTCCTTTCAGAGTCGTCTCAGCATCCT

TCAAACATCCCATTTCCACCAAGAAGAAGAAAGGCGAACTTACCCCATCCGCTGGCAGACCGCATATCTGGGTGTCGTTGATGTTGCACACATAACCGGAGGCACAGGTGCCCACCGAATCCTGGACGGTATCTGTGCCCAGGCAGAGGGCGAAGCTCTGGGTTCCGGTGCAGGCGAACGTCTGGGTCTCATCGCACTTGTTGCACTCCTGGCAGTCCGCCTGACTGTCAGATGCCGAGTCCTCCGGCTGGCAGAGCACACCGCTGGAGCCGCTGACGCAGACATAGCCGCTGGGGCAATCGGTGGGCGTGGCCGTGGACAGGACGTCCACCTGGCAGGACTGCATCTGGGTCGAGGAGTAGCAGGTCAGATTGTTGACGGTGTTGCAGACGTTGCAGGTGCCATGAGTCGAGGACACAAGGCAACCGAGCAGGAAAAGCAACAGCGGCAGGCCAAGTGGCAAGCAAACACGGTTAGGCGACATTTCGGGTTGCAATTGTTTTTTCCGACTGATGTCTGTGCCTGGCAGGCATGTGGCTTAAATAGAGCGCTTTTTTGGCAGCTATTTTGGAGCATGCAGATTGCTCTTAATCCACAAAGACCTCGGCAGGCGGGACCTTGGCTACTGCTCCAATTTAGATAAGCTCTTGCTGCTTTGACGGACTGAAAAGGGTAGCATACTTTTTGGTGTTGGCGAATTTTTATTGGGTTATTTTTGTTGTATGTTTACATAGTAATATAATAGCGATATATAAAACTGAATATGTATGAAACCTGTTAGGCGACAAGGATAAATAGATATCACTAGCTTTTTTACTACTCAAATAGCCTTATCTTAAATAGCTTTCTGGATAAGAAAGTTGCCTATATAGTTTGTAAAGTTTGAATATACATGCAGTTACTCTTGAAAATGCAACGGCACCTGCTCTTTTC

 

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