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CRIMIC CR01727

Gene ABCB7

CG7955

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:1623413..1618414	62A8-62A9	FBti0212959	91287

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGAAAACAGTCTGCGTGTGCGTTGTT	CTTGGCAAACACTGCATTCCGCAACT

Images

Construct Design Sequences

sgRNA sequence	TGGAGACGCCGAGCAGTTTCAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGGATGAGCTCATTCGCCTG	GTCTCCAAAGGCAATCTGGT	GAGCTGTCGTACCTCGTAGA	CATCGTGTCCACAGCTCCTT

 

Amplicon 5'

Amplicon 3'

AGGATGAGCTCATTCGCCTGCCCATTGGCTGTCTCATAAGCATAGCGACTCTGGAATCTGATGGGCCATTCAAAAGTCTTTTCGACGAAGAGTTATTTGACAAGGACTCCAGAATTTCCTTTCAGGCACTGATAGCCTGTTATATTTTGTACCACAAGCACTTGCAGGAGTGTACGCTAGGGACTCACTCCTCTACTTACGCTGCATATTTGGACACCCTGCCCAGGTGCTACTCCACTCCGTATTTCTGCTCCATACCCGAGCTACAGTGTCTGCCAGAATCCCTGCTAGAGCGGACCGTGGCCCAGAACCGTCAGATAAGAGGTTACTTTGAAATTATTAAGAACATTGTACACAAATGCGATTGCTGTGGGAAGAGCTACGGCCAGGAGATTTGGACACTCGCAGACTTTAAGTGGGCCTACTTCAGCGTGAACACTAGGAGCGTCCACCTCAGTTCCCGCTTTCTGAAAAAGCAGAGCAACTACTTTCAGCCGCTTATCAGTGGCGACACCAACCTGGCATTGGCTCCGTTTCTGGATCTTTTTAACCACTCAGATCAAGTGGAAATCACAGCAGGGATTGAGGGTCCCGATTACGTTCTTACATTGAAATCTCTGCCATTTTCCGAGACCAAACCATATGATCAATTGTTCATCAGCTACGGTGCTTTGCCCAACTTTAAGCTATTGACGGAATATGGCTTTTGGCTAAGAGAAAATAAGCACGATTACTTTGAAGTCTCCCTACTGGACATTGAGCACATGATTAAGCAAAACAAGGAGCTATCAACGCAAACCTTTCACCGGAACATCTTTAAATTCATCAGGGAGCACAATCTGAACGATCAAATGTTCGTGCACATCGACGATGGATGCTCTCACAATCTAAGGGTTGTGCTGCACCTGATCTTCAAGCAGCAGGCCTACTTTCCCAACATCCTCAACCAGATTGCCTTTGGAGAC

GAGCTGTCGTACCTCGTAGAATATAAAATAAAGGAATACGAGATCTTTGGAGGAGAGCTGGATAAGCTTCCTAAATTGACAGAAAGTGGTGCAGTGGCACGGTCTTACCTCCAAGAGTGTATCCGTTACCTCGTTGACTTTCAATCGGTGCATCTAGCTGCATAGAAGCTGTCTAGGCTGAGCTTAGAATTTAAAACAAGGCGTTTAATATTGAAATGAATAGTAATAGATGTTAAAGAAAAACTACTTAAGTTTATATTATTTGAGGCATTTAAATTTTTTTTAATAAATCACAGAAAACTGTAAACTTTATTTTGTAAATGACCCCTTTCAGCTGTACTCTTCGCTGACTAAGGTTGATGATAAGAACAGCAGCGATGCCAAGCGGAAGAAGATTACCCCGTTTACGCCCACGCCGGGTAGCAAGCTCCTGGGTGGAGTATTCGGCAAGAAGGCCAAAGGTAGGTGCCGTGGGGCAGCATCAGTTACCCAGTAAATAACCAATGCTCGAGTATCCTGATTAATACAGCACCCCTGCAGATATCCGCAGGCTTTTTATTTAGTACTAGCCTCTCACGCTTTTTTTTTATTGACTAACCAACCCAAAATTGATTTTGTTTTGTGGACCGAGTACGTGCGTCCGTTTGTTTCTTTGTTTGTTTGTGCGCTTGTTGTGTTGAGCGCCTGTCTGAATTTGGCTGCATCCAAGTGAATTCAATGCAGGTGGAGCCCCAGCAGCAGCAGCAGCAAAAACAACAACGCTACAGCGGCAGCGACCTTACCGCCCACAACAACAGCAATCACGACTATGTCATGTTCATATAGGGGGCGGCAGCGATGGTGGCTCGGGATTGGGCAAGCTAGACGCGCCGGAGGTCACCTCCAAGGATATGCTGCGTGCCATGATGGCCTACATCTGGCCAAAGGAGGATCCACTGGTGCGAAAGCGGTAAGTTCATCTGACTATGACTGAGAATACTTTAATATCTATTGTTTTCCAAATTGACAGGGTGGGTATTTCCCTGGGTCTGTTGGCTGGCTCCAAGCTGCTGACCGTCTGTGTCCCCTTCTTGTTCAAAGGAGCTGTGGACACGATG

 

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