• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR01728

Gene CG10654

CG10654

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:12507840..12505374	69C4	FBti0212960	91288

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTCTGTGACTTTGGGCTCGACTGTCA	TTAGGGCAGCCTCATCGATTCGTCAA

Images

Construct Design Sequences

sgRNA sequence	TTTGGAGGAGCCATAAATCGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGTTAGATGTGCCTTTCGCG	TGGCTCCTCCAAATAAGAAAGT	GCGTGTTGCCATCTTCCC	ATGTCCCGCAATTCGAAAGC

 

Amplicon 5'

Amplicon 3'

CGTTAGATGTGCCTTTCGCGCAAAGCACTTGCCACACTCCTCACAAATGTGCCGCGCATCCGGGGACTCTGACTCGTGATACCTTTCGTGCGTCCTTCTCATGTGATATTTGAGACTTCGGTTCGCTGTGTACACCTTGTTGCAGCTCGGACAGGCATAGATGATCCGCGCAGCGTCGGCATTGTTATGCATCTGCCGCAGGTGCGACTCCAGCAGATTGGCCCGGGCATAGCTCTTGGCACAATGGACGCAGGTATACGGTCTCAGACCCTCATGCTGCTGCATATGGGCCTCCAGCAGCGACGGTTTGTAGAAGCCACGGTGGCAAATTCGGCACTCGAGGGTGTGTCGCACTCCTCGTCGTTTTCTGGCACCCAGCAACTTGCTAGAAATGGATAGCTTCTCCTGGCCCAGATCCTGTTTGGCTGACTCGTCCTCTATTACGTAGACTTCCTCCTGGAAAATTTAGGGTATTATATATTTAATTACCATAGCAAACTGAATGGTGGTGGTTTTTTTTTTTTTTTGTGGGAATTCTGTACATATCTATTATGAAGGAATTTTTTATGTAAAAAGGGTCTAAGCATATAAAAACAAACTGCTTTTGGTTGAGTTAAGTAATTTGTAGTAAGGGTAGGAATTACATTATGTTTACTCATTACTTTAAAGTGGAAATTTTTCCTTAATTTGCTATTAAGATTATCTTATCGCTCGACTTAGAAGATGATAAAATGAAAGTAATATTTTGTGAACCGAACTTTTGGCGGTCACTTATCTGTTTAGTTTAGTTTTTTAATGCTAAAAATATTAAAGGCGCTATAAATAATATCTAAAATGAGTGAAAGTTAGGGAACACATTTATCTATTCTAGATAAAACTGGCCTTTAAAATCGACTTTCTTATTTGGAGGAGCCA

GCGTGTTGCCATCTTCCCAATTTTATTAGTGGAAAATTTAAAAATACCCAATAGATTGGGACGTGCCTCCTTTTCGAGTGCTATCACCCACCAAACAAAAATAGTACAACACAAACAAACGCTGTCGAAATGTTTTTGTTTATGGCGGGGAATGGGGAGCAGTATTGTCTTTGCTTGGCTGCTAGGCAACACACCTCGAGCGAAGCACCCAATGTGATTCTAGATAAGATTACGGCCAGAGGCAGGCACACTTGTGGCCAGATAAAACTCACAATCTCCTGCGTCGCTGCGATGCAGGGTGCGTGCGACTGGGCTTCCGGATTAGTGGACTCGTTGCTCTCCGAGGGGGTGTCCAAGTCGTGCCAAGTGTGGTCCTCCAGTTTGTGGCATCCGATGTCGATGTCCTGCAGCAGCTTCTCAAAATTGCGCAAGGACTCTGCGCACATGCGTTGAAAGTCGTGGAACTTTTGCACCAACTGATAGCAGCACTCGCAGATGCTTTTCAGCACCAACTGGGGTGGCAAATCCCTAACGGCATCCTCTCCAGTACAGCCGTAGTACTTGCTGGCCAGATCCTGTTCGCTGTCCAGATTGTGACACGCATCCTGCGGACCCAGTAGCACAAGACAAGCGCGGCATATCATCGTCTTGGCACCATCGCATCCATCCATGCCGGAGGTAGTGGTGGTGGTGGAATCGGCGACAATGCGACCCGCGCACAAACTCAGCTGGGAATCAAACATCGTTGCTGGCATCGTCGGGAGATTCAACGGGCCACTCAAATGGCGTGGAGCCAATTGAAATCAATTAACACAATCTGCGACGGCGGCAGTGCAGCAGTCCGGCGTCCAGCGGCCAAGCCAACGATGGCAGTAATGCCAGCGGAGAGCACGATTCCATTTCCGGGGCTTTCGAATTGCGGGACAT

 

---

Search Again