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CRIMIC CR01730

Gene CG30046

CG30046

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:12314558..12314562	48F7	FBti0210483	86496

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCTTCACCACGGACAACTGTGATCAG	TCCCACTTACAGTCCTCATCTGCACA

Images

Construct Design Sequences

sgRNA sequence	AATCAAAGCCGGGCCCTTATCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATCAACCGGTTGGAAGGTCC	TATCGGCGGCGACCTTGAAT	GCCCGGCTTTGATTGTTTCC	TAGGTGGGACCCGAATCTGT

 

Amplicon 5'

Amplicon 3'

ATCAACCGGTTGGAAGGTCCTATGATCTAGCAGGAGGTAGATCCTATGGAGAAACCAGTGAAGCAGGTGATATTGGAGAACCCATGTCGCAGACCAGGAGCAGATACGACACATCCAGACGCGGTCGGTATGGCGGCTGAACAAATTAAAGCATTTAAGTCGCCTAAATTAGAAGAGAATAAATATTAATTTATGGTATAATCATGACTAACTTGTACTCCAGTTTCATTCATTATTGCATCAAGGGTATCCGACAGTCGAACCAAAATACTATGGCGTTACTTTTGATTTATTTTTAAATGCACTTTCATCCAAAGAGATCTTATGGATTCAATTCTCTTTACTTTTTGGCTTTCCTGCATCGTCGACTGATAAGAGCCCAACGGACTATTTGAATCAGTGGGTCAGAAAAACAATACGCTCTGTCTTATCGGTGGGTTTTGTCGGCTCCGGAGCCTCTCTTCTCGCCGTCTGTTCGGAATTTTCGCTCAGTTTGCAACAGTTGCTCGCGTCGAATGGTTCTACACGGTAACTACGGTCTCCCAAAAAGAACACGAACATGTGGTGGTCAAGTGGTGCTCCTCTCGTGGAGCTACTTCTGCTTGGGTCCCTGGTGATTGGTAGAGTCAGCTCCCTGATATGCACGCGCCGTCCAGCGAACACGGGCTCGCCCAAATCTCCGGTGGATGAGAACTTTATGATAAGCGTGTCGGGTAATCCGGAAACGTACATTCTCGGCCAGGAATACAACGGTGAGTTCGGATGGGTTCACTGGCCTTGCGCTTGAACTTCGTGGGAGATTGTCGCTGGCATGGGACTTTCGGATTATTCTAAGGCTGAATTCGCGCAATGTTTATTGTTTAGTAAACAAAAAGAGGGCCTTATCACGAAACTTTGCAAATTCGTTCGAATTCAAGGTCGCCGCCGATA

GCCCGGCTTTGATTGTTTCCAATACTGAGATTTTAACTCGACTGGTCATTTCTCTTTCAGTCTCTCTTAATGCCTTCAATGGCCACCGCTACATTAGCTTTATCATGGCACTGGAAAACGAGAATGGTGACTTTAGCTACAACGATGACTTGGGTCGCTTTGAGCTGAGCGACTTGATTGAGACCCGGTTCAGTCCGAACTGCATCAACATGGTGGAGAACACCAATACGAACTCCAAGACGCACATGCACTTGACTTGGGTGGCGCCCAGTGAGCCGGGCAGTGGCTGCGTTCTGATCCGGGCCACAGTTCAGCAGCATCGCGAGGTGTGGCACATGGATGATGGCGGTTTAACAAAACGCATCTGCGAGGAGGTCACCGATGATGTGGAAAGTCAGCCAACTGCTCCGGCGGCAGTGGATGTGCCCTGCTGTGCATGCGATGAGGCGCGCTATGAGGTAAGCCCGTACGGAATGACCAATGCCTCAATTACCTTCTACAATTCTCCTCTCATTAGCTTATATTTGAAGGCGTCTGGTCCAGGAACCTGCATCCCAAGGACTTTCCCACTCGAGGCTGGGAGACTCGATTCTGTGAGCTTCTGGGAGCCGCCCACAGTTCGGATTATCGTTTCTGGGAGTCCGGAGAACTGGCCAGCGTGGCTATGAAGGAATATGCGGAACATTGCAGTTCGCGTCTGCTGGAGCGAGAGTTCAGCATTAATTTTCGGGTGACTCCATGCTTGAAACGATTGGTGGGAAGTACTCTATGTTACATCTTCCCTCTTACAGGATCAAAAGATACGGACGATAATCAAGGCACGGGGCCCATCGTATCCCAACATAAGCAGCAAGACAATGGCCTCGGTGCGAGTAGATCCCATTCACCACATGGTGTCCTTTGCCTCCAAGATAGAACCCTCTCCGGATTGGATTGTGGGCGTTACTGGACTGGAACTATGCCTGCGCAACTGCACATGGATGGAGGAAAAAGTGATCAACCTATATCCGTGGGATGTGGGCACAGATTCGGGTCCCACCTA

 

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