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CRIMIC CR01731

Gene ari-2

CG5709

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:22214363..22214390	58D2	FBti0210484	86497

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTCGTGTGTGTGTGTACACATGCATG	CACTTGGTCAGCCACTTCTTGATCAC

Images

Construct Design Sequences

sgRNA sequence	GATATGAGGTATCCGATTGAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGAGTGTCCTCGCCAGAAAC	TTGAGTTAGGGGGCGTCTTG	AACATCGTAACGTCGAGCAC	AGGCTGTAGAACTTGTCGCC

 

Amplicon 5'

Amplicon 3'

TGAGTGTCCTCGCCAGAAACTCGAATTCCCAACCAAACATCCCGAATCTGAGGCCCAAATCTTAAGCGCCAATCCGCCGGAATGGACTCGGACATCGAAATGGATATGGAATCGGACAACGACGGGGAGTACGACGACGACTACGACTACTACAACACAGGTAAGTGGAAACTAGTACCTGATCTAGAGGGGCACTTCCCCCTGACAACAATCGGCCATCGATTTAACTGTGGCCAAATTAGGGGTACGAGTGTATCTGTGAGGAAGTACACACACACATGTCGGGCTCTTGGTTTGGTTATTCACCTTTTCGGAAGGAGGCCCGCAAGACCTGGGAATTTGCATTCAGCACTTGACATTCGCCTTGGGTTCCATGACCAAACCCCACACCCCCCATTTGATCTCCGCCCTTCTTCGCTGACCATGAAGCTTAAATTATGGAATTGAACTTGAAACAAACACACACGCACGTAGCTGCACGGCGAGAAAATTGCACTGTGTAATAGGATAAATGTTTAGTTTTAAAATGTCAAATCTAGGCCAAACTAAATGATTACATTTGGTACATTTCCCAAAGCAATTCTAATGATGATTTGATACCTCAATTTAGAGAATTATGTGATAGGGCTTGATTTTCAAATGGTTTTATAACATGAACTCAAATCAATATTGATTTAAAAGGGGCAAAATAATATAAGAATATTACTAAAGACTTGATTTCTCTCAGTGCATGGGCAAGTACAATTGGTAGTTGCGAGTTGGGACTGATTGCGAGGCTTGTGTTTTGTGGCTGGCTTCATTATTGCCCGCCACTCTGCTCACGCTCTTCGTAGCTCCACAACCCACACAAGCACACATTAACACACACACCACGAACACCAGCTCAGGTGCGTGCTCAAGTACGCAGTTGCATTCTGTTGTGTGCCGCTGTTCCGTCAACTCGGTCTCCGCTTCTTGACCTGTCCCTTATTGTTCCGACCTGGCCAAGACGCCCCCTAACTCAA

AACATCGTAACGTCGAGCACTTCTATGGCAGCCTTGTCGCGCGGTTGTTTTCTCGAATTAGCCAATGTGACTAACAAAATGCAAACATGATTACCGGCTGCATACACTGTAATCCCTTTTGATCCATACATGTCAGATTAACATAGAAGATATTCCCAAAGTAATAATGTTTCGTCGGTTTCAACCGATCAATGTAAGTCAGCCATTGCTTAACTTACAAACTCAACTCAACCTTCGTAACTCTGGAACCAGTTTTGTACAATAAGGGATTAAAAGTGCCCTATTGTATATAACTGCAACTGCTAACTCAGGGTTCGTTTGAAATCCACCCTTGCTGTCTTTCAGTCTGATTTTGGTTTAAAATACAATCAGTTAGCTTAAATAACCAAAAGAAAAAGTGGAAACTGTCCTATCAAAGCATAATAGATTTATGATTTATAAATGTTATAAAGTTCAATTATATTATAATACTAATATCCTTTTCTTCTTCAGGCGAGGACTGCGATGTGGAGCGTCTGGATCCGAAGCGGGCCGACCCGGAGTACTTTGAGTACGAGTGCCTAACCGTCGAGGACATCGAGAAGCTGCTGAACGAGCGCGTGGAGAAGCTGAACACCATCCTGCAGATCACACCCTCGCTGGCCAAGGTGCTGCTGCTGGAGCACCAGTGGAACAATGTGGCCGTCGTTGAAAAGTACCGCCAGGACGCCAATGCACTGCTGGTGACGGCGCGAATCAAGCCGCCATCGGTGGCAGTGACGGATACGGCTTCGACGAGTGCAGCGGCGGCCAGTGCCCAACTGCTCCGACTGGGGAGCAGCGGGTACAAGACGACGGCATCGGCGACACCACAGTACCGCAGCCAGATGTGTCCCGTTTGTGCCAGCTCGCAGCTGGGCGACAAGTTCTACAGCCT

 

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