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CRIMIC CR01732

Gene CG32176

CG32176

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3L:17437971..17433868	74C1-74C2	FBti0212961	91289

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAGAGTTGTCAAAACACGCTCCCCAA	AGGGAACTACGTCTGACAACTGGCTT

Images

Construct Design Sequences

sgRNA sequence	ACCGTACTTAAACTTCTCGCAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACAACAGCGCACAGTGGATA	CGGTGGATAACTTTACGGCG	GACACGTAAAATGCCCGCAG	GGTCTGGTAGCCTCCTACGA

 

Amplicon 5'

Amplicon 3'

ACAACAGCGCACAGTGGATAATACAGTCCTAAAGGCAATATACACCATTGTACATTTTTAATTATTGATTCTATGTGCCATTCCCTAGTATACGAAATAATTTTTATTAAATACATTTTCGTAATACTGCTCGTTTTTCTTTGCGTTTTGAATTTTAAAATAGAGCCATTCACACTAATCCCACAAAATTGTTGCCTTCGTCCCACTGTCCACAAGGTATAAATAGTAGTGGCAACGCTGCCCGAAAGACCGAAATTACTTGGTTTTTATTTTGACAACAAATTGGATAAAATGAGTGGCTTGAAGGCGAAAAAATCCAGCAAGGGCGACCAAATCCCCAAGCCCATTAGCATTAAGAAGGAGAAGATCGAGGAGGAACGAAAGCTGCATCCCATGTCCCATTACATAGACGACCGCCTGGAATTGGTGAAACAGATATTTGGGACCTTAAAACCGAAAACCATTTTGAACCTAGCCCCAGAGTTTCTAAAGGTGTGTTATAGTTCTTTTTATAAAAAGCTATTTATCAATAATTTCACGCAGAAAACGCCACTAGACGAAATCGAGGAACTTTGTCTGGAGGAGTTACTCTGCTTGTCTACGAAGCGGCTAAAATCCATAATAGAAAACACCAAATGTCCCACAGACACTGAAAGCTCCGAAGATTCCGATGTGGAGCACAAAGAAGGTATGTTGAATCACTTCCTTGCCCTATAAACCCACCATTCATTATAAAGTCTCATTTCAGAACACATCTCGCTAGAGGAGATATCATCCGACAGCGACATCGGAGGTCAGGAATCCCGAAAAAGTGAGTTGGTGGCATAAGATACATTTAATTTAGAGTTTATTATCTGTGTAATCTGCACAACTGCACGCCGTAAAGTTATCCACCG

GACACGTAAAATGCCCGCAGGATGCAAACATCCTCTCCTATGCCGATGACCACGTTATCCTTATCCTCTTTGCCCACCTCCAGGGTAACTACAAAGTCTCCAACCTCCAAGCAGCCCTTCTTCCTCATAAAGTCCACTCCAAACTCAATCGGACTGTAGCTGCACTCGTTGAGATCCTTGGTGTAAAGAACCGGGTGCATGTTACGGCGCAAATGAAGCGCACGAGCGAGCTCCACCTTGCAGGTAATGGTTTCCGCATCATCTGTCTCCTCCAGCGGCACCATCACATATACTGGGCAATAGATCTCAATACGCGAAAGGGCCACGCTGGCGGTCTCGCAAGTGGTGAACAAAAATATGGCTTGACACTCAATCGAGCTAATTGTGCGGATAATAAAATTCACAATGGTCTGGTATGAGGTTAGGATGCTCTTCAGTACCATCTTATTCTGGACCGCGCCAACACGATAATCTTTTATGGGCATCAGCTTTTTGACTATCAGCGGATATTTGTTGCACCATGGACTTTTTTGGATGTGAATAGCGTCGACCTTCCACAGGAAATCGTGCAACTCAAATCGCTTAAAATCACTGCAACGCTCCAGCGGAACAGTGCCAATGACTGGCTTCTTCTGGCACTGCGAAATCGGAAGCACGTCCTCCATAATGTAGTTATATACGCAACAGTTTACGTTGAACATGTCGGGTATCCAAATGTAGTCCACAAGCTTAATGATGCCTAGCAAATCAAGCATGTTACTTCGCACATACTCGAAATCTATCATTCCGATCAACTTAAGGTTGAAGTCCGAGTTCAAGGTCTGCCGCACGGCACGAAGAAAGCTCTTGCATCGTATCTTGGGCAGAACCACATAGTTGGCATTCAAAGACGCGGCCATCTCAAGGTCCTTCATGTACAGATCGGGATATAGGTCCGGATCGAGAGCGTGGCACTGTCTAGGAAGCTCTATAAAGTCGTAGGAGGCTACCAGACC

 

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