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CRIMIC CR01734

Gene unc-13-4A

CG32381

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3L:7312896..7266192	65E10-65F4		91525

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	long band	CTTTCCATCAGCTGCCATAACTTGGC	GCATTGAGAGCAATCCAAGGACCCAA

Images

Construct Design Sequences

sgRNA sequence	CGACGCAGTCAAAGTCAATGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TATCTGGCAGCCAAGTTGGG	ACTTTGACTGCGTCGTTCCT	CGATGTGGTTCCGTGTGGT	ACGCCTCTCGCATATGGATC

 

Amplicon 5'

Amplicon 3'

TATCTGGCAGCCAAGTTGGGGCAAAATATTAGCATATTCATCTGTGGGTTAATTACCTCCAGCAAACATGCGCCTCCACCCATTCCATCTGCGACCCAATCTCCGCCAGCAGACAGGCATTAAATTTGCCAAATCCATTGGCACTTCATCAACTATGCATTAAGGGCACCGGACTTGGATTTGGTCTCGGTTCTGGCTATGATTATGACTTTCGACTTACCCGGAATTGGAGCGCGGATCCACTTCGGGCCAGCTCAGGCGGCGTTTGCAGAAGGCCAGCGGCGGACGTGGCGGCGGATCCGGTTGATGCAGTGGCGAATTGCGCTGCCTGAAGGATCCGGTGCGGGGTGGAGCCAGGCATTCCAGGGCCCGTGACTGGCGACGCGACTGGGGCGTGCCACCTGGCCCAACGGAGCCGGATGTCGAGGGACTGCCGGGTCCCGCGGCACCACCTGGTATTTTGGGGTAACCTTTTTGGGGAAACATGAGAAGGGGTAAAAGCATGAGCTGAATTACATAAATCAATATGGAGCAGGTGAGCAAAAGTTAATATACTTATTTACAATAATATGGTTTGTATTTGGTTTGACTTTCATGCAAAAATATAAATCTTCAAAAGGTACAAAGAAAATAGACACGTGTTTGTTTTCCCTTAGTTTTCACCATGCTTCTGTGAACTTTCTTCACATCGAAATAAGAAGATTGAACAAAGTCATACGAAAAGTTTGATCTTCGAAACTATTTGCCCTGTGCAATATCCTATAATTTCCCGCAACATGCAAATATCTATGGAATACAGCTAGCTGATGGCACCGCGCTCATGGAAAGTCAGGTGTGTAAGGAACGACGCAGTCAAAGT

CGATGTGGTTCCGTGTGGTGCAGTGGTCCGTGCTGAAGGCAACGGTAGTGGCAACTGCAACGGCAATGCAACCGCAGCCCGCAACCTTGACATGCATCTGTGTGGGTGCTGCGGCTCCTGCAGCTGGTCCTGGTTGCCACTCCTGGCTGCCAAGGACTATGACTACGGAGGCAGAGAGTGCCTGTGGTGCGGGGTTTCGGATGGAAATAGCTAACCATTGATGTGGCTATGAAAAGAATGTTTTATTTTTTTTTGTCTGGCCAAGTAGCGGTGAAGCCGGGCCAACTAAAAAATTTCAAAGAAAAGCAAAGAGAAGAATACGAATATGCGCTCGAATTTCGGTTTGGTTTAAAAGTGTGCACAAGGCGTAAAGTCAAAGTTGGACTGCATTGGACTGCGGAATTGGGAAAAGCGCTTACACACGTTGATGGGATATTTTTTTTTTTTTTTTTCATGGAAACGACAATTCCCCGTTGGCAGCTGTTTGGCCAGGAAAAACCGCTCGTTCGCCGCTCGTGCAACAAGATAAGCCAATTTCAAGTTTGATTCAAATGCTGCAGACGTCGGTGAATCAAAATCCCAGCCAAATGGCAAGTCAATGTCGTTGGCAATGGCGATGGGCATGTGATGGCTATGGCGGAAAGTTCAGGATCCGGCGGTTTGTGGTCCTGCTGATGCTGATGCCACTGCTGCGTCTTGACATTTAAATGTGTTTGCATTTGCCAACTGCTATAGAAACAACAACCTTGTACATCGTTTAGCTTGGCAAACAAGTTTTGCATAGCCAAACGGAGCCAAAGCGGAAACGAAGCCGCACAAGAAGTCACATATGCATGCTGATTGCTTTCGCTTCGCCAGCTATCATACACTGAGAGAAACAAGTGCTGCACGAGCAAGATAGTTTTGTTTATTTAAATTAAAGATAGAAGATCCTAATAGTTGTGCTCTTATATCTTTAGATGCAATTTAGTTGGCTTGGAATGGTTTTTGCTATTATACAAATTTTTTCCAAATTTTAATTCTTTGTGTATAAGCCACTATCAGCCACCAGAACCAACTGGCACCCCATTGCGGTGGTTTTGTCTGCACATTTGTTGATCCATATGCGAGAGGCGT

 

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