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CRIMIC CR01735

Gene Spt20

CG17689

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3L:13511541..13502707	70B3-70B4	FBti0212963	91291

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCAGTGGCTTTGCTGTGCCAAACAAT	CAGACCGATTGTGTTTCGGCTCCAAT

Images

Construct Design Sequences

sgRNA sequence	GGATTAGTTTAGTTGCATGAGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCCATGTCTAGTCCGCATCC	GAAACGAGATGTTAACGGGTTTG	TCCAATCCGATTTGTGTGTCT	GTCGTCTCAGTTCCAGGCAA

 

Amplicon 5'

Amplicon 3'

CCCATGTCTAGTCCGCATCCGCAGCCCTCTGCCTCGCCGCAGCAGCAACAACAGCAGCAGCAGCAACAGACCACCGCCACTTTGAATCTGCAGGGCATCAATCTGTCTCAACTGCAGGGCGCTATGGCTAACTTTGCCGGCCTGCAGAATGTCCAGGTGCAGATCCCCGGCTTCACACAGCCCATTTCGCTGCAGTTCTCCGGTAACAGTTTGCAGCAACAACAGTCGGGTAATACGGCTACTGGTGCGGGCACAGCGCAGGGCCAACAGAGAAGTGTCCTGGTCTCGGTTCCAGTCTCCAGCCAACAACAGCAACTGCCACACACCATAACGCTGCAGACTCAGTCGGCGCCGCACCACCAGCAGCAGCATCAGCAGACTCAGCAGCAACATGCCCCACCAACGGGCACCATTGTGAGCCTGCCATCGTCGGGACCTGGCACACAGACGGTCGTCATTACGAATAACTCCGGCGGAGGAGTGACGGCGGGAAGCAGCACCAGTGGCAGTGCGGGCGGCGGTGGAGGATCAGGGGCCACAACGGCCATGCTCACCCTTCCCATTGGTGAGTATAAGAGAAATGCGTTCCTGGTGCAGAGGCCGCTTGCTAAGAATGCTGCACTGTCGTTTCGGCCAGGCCGAAGAGATATTTGTGACTCTGGATCTGGCTTCTTGGTGGGAATCTTTATTTTATAATCGGACCATATTATTTATACGTAACTCTTTTCTTTTAATTGAACATGCTTAATTTTGTTCTTTTCTGTTTCTGTTTCGTTTCTCGTTTACAACATCATACAACACAAACCCGTTAACATCTCGTTTC

TCCAATCCGATTTGTGTGTCTTGTGTGTCCTAACCCGAAATGCCATCAGTTTGAGATTAGAGGGTCCATTATGTATAGCCGCAACCGTACTGTACGTACTGGTTTGGCGACCAAGTTGGCGGCTCCCCGACCTCTGACCTTCAACATTTCTCATTGCAACCTAAAACAACAACCAAAAAAAATCTCCCCGCACACCCACGTTCTGCTCTCTCTCTGCTTACAGCTCAAATAGTCGGTGCCGGTGTACAGAAACTAAATCCTCAAACAATACGTACCTCAAATGTTGGTGGTGGTATAGGTATCGCCCAGCAAACGGTCCAGCAGCAGACGATTCAGTCGCAGTCGGGCGGCAACAGCACCGCGGCCATCCAGCTGCTGGGCACCATACAGCCGCGTGCCCGCAACGTCCAGGTGGTGGGCACCAAGCAATTGGCCAACAGGCAGCTCATCACCACCCAGCGGCAGATAGGTGGCTCCACGCTTAAGATCGCCACCACACCCGTGAACGGTTAGTAGAAATCCATTCATTTCTGTCGACTATTACAAGCGCATATGTTTTTCCCACTGTTAGCTGCCAATGTGGTGACCAGCAGCGCCAACCTAACCACCACTCCGATTGTGATGTCCGCACAGAAGCTGCAATTGAAAACGGTGAAGGCGCCCGTGCAACAGCAGCAACAGCCTCAGCAACAGCAACAGCAGCTGCAGCAACAGCACCGAATACTCAACCAGCAGAGCACGGCCACGGTGTCGTCGCAAACACTGCCCAGTCCCAACCAAGTGCAGGTGGCCCAGCAGCAGCAGCAGCAGCAACAGCAGCTGCAGCACATTCAGATCGCCGGTAGGGCCACCACCGCTTCGGGAGCCATCAGTCAAAGGACTCTAACAGCTCTGGCGGCTGCGAAGCAGCAGCAGCAGCAACAACAGGCTGCGGTCAATCGACGGCGCTCCACCACCGATGCGACCAAGTAAGGGATGTGAATTGCCTGGAACTGAGACGAC

 

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