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CRIMIC CR01742

Gene Ca-Ma2d

CG42817

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:16234091..16234114	35F1	FBti0210486	86499

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTCTGGCTCTCTAGACTTTTCTCCCC	TCAACCACCTGTCCGAGTTCCACTAA

Images

Construct Design Sequences

sgRNA sequence	CATTGGTGGGGCATATATCGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GTGAACGAAAGCTTACGGCC	CCCACCAATGTGCCCCAATA	GCTGGCGGAATAACTGTGTC	ATGTTTTCGGGCCGATTCCA

 

Amplicon 5'

Amplicon 3'

GTGAACGAAAGCTTACGGCCCTCATGATTAATACAAACCAAATATATTTTTAGTGATTTTGCAATGAAAAGCAATATATAACAGAATGGTTCATATATCAATGATTTATATATGTATCATTTTAAAAACCAGAAAAGATTATAGGTATAGTTATCCTTGCGTAAATACGCAAACAAGTCTAAAAGACTAATAAAAAGTTTTGCGGCCGACATAAATTATAATAAAGTAAATGTCCAATTAGCGAACTACACCATGGATAGATAAATAATCAAATATTAAAGACCAAAAACCCCATAATAAAATTTCCCCAACCAAATTAGCTCTACTAAATAGCCACAAAATTGCGGCATGGAAAAGAGCAGAGACCGAAAATCGTTTAATGTCCGTTCGCATTACAAAGAATATTTAATGGGAAATCGGGATTTTTGGCGCATTGCAGCCAGTCGAGCTTTAATAGAATAAAGCAATTTATGCCCGAGGGAAGCGTAATAAAAATTCAATCTTAAACAGAAAATATTGTTCAAATATGCGCGTATTAAATGTTTCCAGGGAAATTTTGTATGTGGTGAAGAGATTTCATTTTCGTTAGCGATCCCCTGACTCCTGTAAGTGATACATTTTCCCCGAGTGCTTAGGGTTTTCCCATTTTCCGTGGCCGCGGCTAACAAGGGCCATCTAAAACAATGACAAACGAGATACGACATAGCAATATGGGCAAATTAATTGAACAACTCCAGAACGCAATAAAAGCCAGAGAGAAGGCAACGTTCATTTTCAACTACCAAACAGCGGCAAAAACAATTTCACCGACCGGGCCAAAACACTTGAGAGTGTGCGGGGAATATATATATATATATATTGGGGCACATTGGTGGG

GCTGGCGGAATAACTGTGTCAAAGGCAACGTCGGCGACAGAAGTGAAATGAAAATGGCCGAAACAGCGCCGCCGGCTGCGTCGGCGACATCAAAAGCCACGTCAAGAGATTTCCCGCACTGAAAAGAGCTAAAGAGCGGATAGAAAATAGTGCGGTCATGCGCAGGCATCAGCCCAGAATGCTTTTAAATGTTTATAGTTAATGCCAAACTCTCACGCAAACGGTTGTCAGGCAAATGCAGTTGGCTAAATAAATTATTTACACGTTTTTTTAGTATACAGTAACATAAAAAATACTGTGACTAAAAACATAAAAATGCATGTTTGCTTATGTCTTTTTTAACCGAGTTATCTAATGCCAAGTAGCCTTTGTGTATGCTTTTTAAGTTAATGCAATATGGCTTAAAGGGCTTTGATATTTTGCGGGGAAATGTAAATATTTAGACTTCACACACAGACTGATTGCCAAAAAGAAAATCATTGAAAGTAATGATATTTATCGCCCTATATTACAATTAGTTGTTTCCAAGTCTTTTAACAAAACTTTTTAAATTTGATAAGAATACGCTACTTAAATGTTCATATTTTCTAATCAGTGGTATTTAGATTTTTGTGTTATTTTGTAATTAATCTTTCTAATAACTATAAAGTTAATTTTTTTTTATTAATTGGAAAGAATATAGATCTAATAACCTACTATAATACATTTGAGATAGAACCACTTAATGCCTAAACATTAGCAACCTATCTTTTGATTATTTTTTGCTATTCGAATGCATTAACTAATGGAATCGGCCCGAAAACAT

 

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