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CRIMIC CR01743

Gene ppk18

CG44152

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:9768194..9768212	30C8	FBti0210487	86500

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTGATAGCGACCTTCTCGTCGATCTC	GGTGATGGAATCTATTGGAGTCGTGG

Images

Construct Design Sequences

sgRNA sequence	CCCATAAGCCCAATCCATACTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AAGGCGAATCAACTGGAGGG	CCGGATATATGGCATCGTACACA	GATTGGGCTTATGGGGTGCT	ACCAACCAGATGAACGCCAT

 

Amplicon 5'

Amplicon 3'

AAGGCGAATCAACTGGAGGGACTGCGATCTTTGGAGGCGAATAGCGAGGATGCTCTGGTCCTAAACAATCTGCATCTTGGGCCCACGGATTTTCTGGTGGCGCCCGACTTCGATGGAACCTTTCTGAAACTCGTCAAAGATTTGGATCTCTCGCCTGAACTCATCGACGCGAATGCCCAAACGAGTCTATCGCTGGACATAGAGCCCATTGCGGATGCTAATCGGCGAAGTGATGACTACTCATGGAGTGAATACCACGAACTTAACGACACGCATCGCTGGATGCAGAATCTGGTGGGGAAGTATCCGGATGTCGTCAGTGTATTTGTGGCGGGTCAGAGTTACGAGGGTCGTGAGCTGCTGGGCCTGAGGATTAATCATAACGATGGAAGAGCTGAGAAGCAATCCATATTCTTGGAGGCCGGAATGCATGCTAGGGAATGGATTGGTCCTGCCACGGCCACCTACTTTGCTAACGAGCTACTCTCCTCTCAGCAGCAGGAGATCATGAATCTAGCCAGATCCTATGTTTGGTACATATTGCCCCATGCCAATCCAGACGGTTATGTCTACACCCACAAAACGGTAAGCTGGTACTGCAACTGATATCCAATAAGATATTAACCATTTTCCCTTTAGAATCGCATGTGGCGCAAGACAAGGAGTCCGCAGGACAAGAACTGCGTTGGAACGGATCCCAATCGTAACTGGGACTTCCACTGGCGTGAGGTGGGAGCCAGTTCTGATCCCTGCTCGGAGTCATATGCTGGACCCAAGGCGTTCTCCGAACCTGAAGTTCAGACTCTTTCTCAGTTTTTGAAATCGGTGCCGGAACCCATGTTCATGTTCCTCTCGCTGCACTCCTTCTCCCAATTGTTGCTATATCCCTATGGACACACATCCGCACTGCCGGAAAATCATAGGCAGTTGGAGCAGATCTTCAATACAGCCGTGGGTGCAATGAAGCGGCGTTATGGAACTCGGTACACTGGAGGAAATGTGTACGATGCCATATATCCGG

GATTGGGCTTATGGGGTGCTCAACGTTAAGTACTCCTTTACCTACGAGCTACGACCGTCTGGGTATAGTTTCTGGACAGGATTTAGATTGCCCGCCGCCCAAATTATACCAACGGGTCAGGAGACCACAGATTCGTTGGTGGAAATGATTAAGGCTGCTGCTCAAATCGAGGGTTACAAAATTGAGTAGAAGAGAGAGCTTACGACGTTTTTGTTGATAAGATATGAATGAAAAGATCTGTTACTTCTGAAAAAAACTAAACGTAAGCATGTTTTGATTAAAAAGATAACTTGGACAAGCTGATAAGATGAAAAGTCCAAATTGGCTTAAAAAGTTTTGGGTAAAAAAAATGATTTGATTATATATTATCAATTTTGCGGACTGAAATGTTAGTTTGTGGAACAGAATGACTTGTCAAAGTTAGAATATAAACAAAAGCTAGAGCTTATAAATTTGGTTTAATGATGAATATAACTAATACTAAAAATGTAATTTATAAATTTCAAATATTAAGACAACGCTTTTTTGAAATTCTTTTAAAGAGTTGGCATAATTAAACTTAGCCACTATCACACACACTTAACATTTCACCTGCCAGACTTCGGAATTATTTAATGATGAAATGTCACTTCTTAAAGAGCTCAAGACGTCATGAACCGAGAGCAGGTCGGGAATTATAGTGATTTAAAACCTGCAGCCGTTAAGACGGCGATAATTTTGTACACCGATTACTCCAATATTTACTGCCCAAGCAATTGTCAAAGGCAGTCGCGGACCGTAAATTAGATTCGGATAATGAATTGTGGAAGCTCTTATCGAAGGCCTATAAAAGGAAGTGGCTAGCCGAGTGTTGGCTCAAACCAACGACAACGATGTCGCTGAACAAGTGCCTTCTATTCGCCCTCCTGGCCATCGTGGCCAGCGCATCCGTGAGCGCGGAACGTGTTCGCTATGACAACTACCGCATGTACAAGGTCAACTCGGAGAATGCCAAGCAACTGGAGGTGCTGAAGGATCTCGAGGGATCCAGCGACTCAATCATGTTCTTGGATGGCGTTCATCTGGTTGGT

 

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