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CRIMIC CR01744

Gene pyr

CG13194

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:11726345..11712263	48C2	FBti0212964	91292

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TAGTTCCGTCTCTTTCGCTCTGTCTG	TTCGATATCGCTTCTATGGCCAGTCG

Images

Construct Design Sequences

sgRNA sequence	CAGAGTCAGTTGAGTACTCGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGACACACCGAGCAAAACAC	GACAGTTGCCAGTTTTGTGT	GTGGCACAAAGTACGTTTCGT	CCTCTGGTTAGTGTGCCCTG

 

Amplicon 5'

Amplicon 3'

GGACACACCGAGCAAAACACGTGATGTTTATAATCATTAATAAAGTTAACAATACAATACTGCATTCAATGCGAACCATTAATCAAACCAACTGGCAAACAATTTATGTTTATTAACAGTGCACACGTCTTTATCACAACTAAACTTTCTTTTTTCTAAGGCTTAAGCCTTCTTCGCAGTGGTAGATTCTCAAAAACTTTAAATTTAAATATATAAATAATATTTCCTGAATCGAGTACATTTTTTCTCAGTGCATCCAAAGGTGAGAGGGCGGCTTGCTAATGAGGCGAGATTTGTGTGGCGGCACGTCGACTTTTTCAGCATTCCAACACTTTTCTGCCACCTTCCCCGCAGCCTTGACATCAAATGATTCGTCTCCTATATATATCTACTGAACAGTCTGAAAACAACATTAGCTTATCGCTGGGGTACACTGAACTCTCACGGATTCCCGTTCCCATTCCCAGTGCTCCGGTGGTCTGATGGTGGTGGTGCACCCCGCTCTATTATATTTGGGGCGTTGCTTTGGTGTCTCCAAGTTCAAGGGGTTTTTGTGGGGGCCGCGGGGGAAATAGCCGAGATGAATGGGAAAGAGCACTCTTGGTGCCAAAAGTATGCAAAGTTTTTGGCATGCTGCGCGCAATCTCAAGTCTTAACTCTTAATTAAAGTGGTCTGGAAAGGGGGCAAGAAAGTGTGCTGGAATTACTTAAAGCTTAAAGATTGTGTTTCTTAAATTGGTTAGAAGTTTTAAAAGCAATGGAAATTACTGATAATGGCATTGAGCGTTTTGCCATTTTCTGCCACACAATTTTTTGTTTGATCCCGCCCAACTCTTGATTTGCATATTGTTCTACATCTCATGTCGCATTGAAATGCATACTTCACATTCCCAATCCGAAACTGAATCCGATCCAATCCTACTGCCTGTTGCAGTTGCAACTGCAGTCGCTGCTGTGCACATTCCTTGCACATATAAATATTTTCAAATAATGCCCACCAGCTCGGCGCCCTGCTGTAAAAACAATGGCAACACAAAACTGGCAACTGTC

GTGGCACAAAGTACGTTTCGTATACATCCCTTCCAGTTGGAACTTCATTAAGTCGGGACACAAGTCGGGGCAAACACGTAAAAGTTGCATTTGCAGCCGGAGAAATCGGAATCGTGGTTCGAGGGATTTCGAGGGGTTACCGAGGGATGAGGCTTAGGGTTGCTCGGCGAAAAGCGCAAGGTGGATGATGTAAGAGTATTAAGTAACAGCCAGTTCCCCGTTCGAGCCACCCCTTAAAACAAAGGAAAAAAAATTGCATTAAGTGCAGCCGGGCAACCGTGCAACCGGGCACACCGAATAAAATAAAAAACTGCACAAAAAATGAAACAGAGGGTGCCCTTAATCATCATCATCATCGTAATCATGATCGTACTCATCGTCATTGCAATCGAAATCATTCGCACAATTGACAGCATAAAATTTCACACCATAACACGTGGCAAGAGGGCAAAAGGGAAAATCAGGACCATAATTACACGATGGATTACCCACAACACGTGCCCGCAATCAGAGTACAATGCTATCACTTTCCACGCCGACTATCTGGCAAATCTTTGTGCCAATTCATTTTTGTGTCCAAATACTTTATGGCCTAATCTAATCGGCTGCGCATTCCACAGATGCCATATGTATTTCGTATATATATATATGTATATGTATGTGTATACGAACAATTATGCGGCTATAAGTGAGCTGGAAGCAACAAGTGTTAAAGTCTAGATGCCAACACGTGCCCCGTTCTCGAGAGTCGAAGATTCGAGGCCCAAACTAGTTTGCATTCGATTTCAATCGAATGGAAAATAAAGAGACCCGCTGCTTAGCATATTATCGATCGATGCGATCGCGGCGAAGCACTTGGAGTTTAATTTTAATTTTAGTTACCAGATTTATAAGTATACAGCCGGTGGCCGAAAGCCCCGTACAATCGAGTGGACAAACTAATTAAAGGCCTGAAAATGTCGCCGGCGATTAGATTGCACATGTCGAATGTGGTCGCTGTGACGTCGATGGTATTACGAAAGGAGGTTCCACTGTTTCGATTTCGAGACATTGTGAATGAAAGCTCAACGTACGGGGTGGTCCAAGCAAAAAATAAGAGAAATGAAAAAAATGTGAGCACGTAGCGAAAGGAAAACTCTCAATCCAAAACTATTGCTATTGAGTATTCAGGGCACACTAACCAGAGG

 

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