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CRIMIC CR01745

Gene CG8788

CG8788

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:9140981..9141010	45A11	FBti0210488	86501

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATGAAGCACAAAGAGCCTCCATCGGT	TTTGTGGCACTCATCTTTCCGCAGTC

Images

Construct Design Sequences

sgRNA sequence	GAACGGCGGCTACGCTCCTCTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GAGGGTCGCACCATGGATAG	CCCACTGATCGAGCTTAACCC	GCCGTTCTCTAGCCGATGTT	ACGGACTTTGCTAGTTGGGG

 

Amplicon 5'

Amplicon 3'

GAGGGTCGCACCATGGATAGCGTCAAGTCGTTCTTCGCCGGCTCCACCAAGGATGGCAGCCTGTCCAGCAACAGGCAGGCCAATGTGCCCGGCTCCTCAGTCGCCGGCCGCTGGGGTCGCAGCTGGAGCACCAGCTCCCAGCACACGGATCCCGAGCCAGGAGCCACCAGCTCGGTCTCTAGCAAGAGCGCCAATCCGTGCGATGCGAAGCGCAAGGACAGTGACAACTACTTCTACATCATGTGAGTTGGTTGGAGTAAACCTCTTTTAAGGGCCAGATTAGATAGGAGGAATTTGAGTTGACTGACATCTTTATTTGTGTTCCCGTTGGGCAGTTCCAAATTGGAAATAAGAATAGATACCACTACTCAGAAAATAAATAGTTAAGCACTAATTAGAATGATTCATAGTAGATTAGCTTGTTAATCAAAAGATCTTTGTATTCAAGGGATGAATGAATAAGAAATTGTAATCAAGATAAGATTGAAATGATCCTTTTAATCTTTTATATCATTGAAGCAGGTTTCCCACATAGTTACGTAGCCTCAGAGAGTAACTTACTTGTATCTTACTTATATACATATACATATATGGAAAGTATAAAGTATATATAGTGGATAATCTGGTCAAATAGCTCGATCAATGACCCCAATGTTGTTATATAAACCAGTCTAGTAGCACACAGGGTACCTTTTCCCTTCGTATTCCCTAGTTTCTCCATTTCTATCACGTTTTTGGAGCAGTTCCTATGTAGTTTATGGCCCTGTTTACTTGGCACAGGGAATATTTTGATTATCTTGCAACCGCCTTATCAGACGTAGTAGCCCATGAGTAAATGGCTTCGGAGGGTGCAGTAATTTCCGACCAGATAGCCGTTCAATGCCTGCAATTTTCGAATGAACAGGGGTTAAGCTCGATCAGTGGG

GCCGTTCTCTAGCCGATGTTCTGGCAAACCAGTTTGTGCGGTCATAGATTCATTCGTTGGCTCACCTTGAACGTAGATCGCTCGGCCAGGTTGGCTCTGAGCTCTGAGCGAGGTGGGCGATATTGATATTTTGGGGGGATCTGGTCTGGAACTGCATAACCAGTTAAGAATCAGTTGCGAATTAACTGGCGCTGCGAACAGCTGAGTAAGCCACGATCGGGGAATGTTAGTGTCCGGGGATAGCCCCGAAAATAACCAATCTGGGCGTGGGACTTGCCAGGTGGTGTGTACATAAGTGCGTGATTCAGTGGAGCAGCTGGGCAGTTACCATTGGGCATTCTCTCAGCACGCTGTAAGATTCGCGATCGCGTTAGCAATTGCCATACCACAATTGATGCAATAACAACTCTCGTTTCATTTCGTTTAAGGTGGCGCGCTTGAAGATTAACAGATGATTGTGGATCGGAGCACCAGTCGGATGAGTCCTGATCCGTGTCTAAAGTGACCTGCAGCTATATATATATATCTGATACCCTATTAGGAACATCGGAGCGTTGTCACTGGCAACAACCGCCTTTTGCCTTGTTGCACCATCTCCATTGGACGAGTCCGGTGTTTAGTGATACTAGTTATTTATTTACGATTTTACACAAGACATTCATTGTACGTGCTGTCCGATCGATCCCTTCTCCTCCTCCTTCTATATGGACGCCATCACAATCCTGGGCTTGATCTACTGGCTGATCTGCATGATTGCGTTCGGACCACTGATGTTCTTCGTCTGCGTTTACCTGCCAGAGGTTCCGGTGCGATATGTAAAGGGTCTGAGGCAGCAGACCTGAGCGCCTCCATCCTACTTGACTACCCCCAACTAGCAAAGTCCGT

 

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